Unveiling RNA structure-mediated regulations of RNA stability in wheat

Abstract Despite the critical role of mRNA stability in post-transcriptional gene regulation, research on this topic in wheat, a vital agricultural crop, remains unclear. Our study investigated the mRNA decay landscape of durum wheat (Triticum turgidum L. ssp. durum, BBAA), revealing subgenomic asym...

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Main Authors: Haidan Wu, Haopeng Yu, Yueying Zhang, Bibo Yang, Wenqing Sun, Lanying Ren, Yuchen Li, Qianqian Li, Bao Liu, Yiliang Ding, Huakun Zhang
Format: Article
Language:English
Published: Nature Portfolio 2024-11-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-024-54172-7
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author Haidan Wu
Haopeng Yu
Yueying Zhang
Bibo Yang
Wenqing Sun
Lanying Ren
Yuchen Li
Qianqian Li
Bao Liu
Yiliang Ding
Huakun Zhang
author_facet Haidan Wu
Haopeng Yu
Yueying Zhang
Bibo Yang
Wenqing Sun
Lanying Ren
Yuchen Li
Qianqian Li
Bao Liu
Yiliang Ding
Huakun Zhang
author_sort Haidan Wu
collection DOAJ
description Abstract Despite the critical role of mRNA stability in post-transcriptional gene regulation, research on this topic in wheat, a vital agricultural crop, remains unclear. Our study investigated the mRNA decay landscape of durum wheat (Triticum turgidum L. ssp. durum, BBAA), revealing subgenomic asymmetry in mRNA stability and its impact on steady-state mRNA abundance. Our findings indicate that the 3’ UTR structure and homoeolog preference for RNA structural motifs can influence mRNA stability, leading to subgenomic RNA decay imbalance. Furthermore, single-nucleotide variations (SNVs) selected for RNA structural motifs during domestication can cause variations in subgenomic mRNA stability and subsequent changes in steady-state expression levels. Our research on the transcriptome stability of polyploid wheat highlights the regulatory role of non-coding region structures in mRNA stability, and how domestication shaped RNA structure, altering subgenomic mRNA stability. These results illustrate the importance of RNA structure-mediated post-transcriptional gene regulation in wheat and pave the way for its potential use in crop improvement.
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spelling doaj-art-414e91c3f6fb427db81b0682b34c0f5a2025-08-20T02:33:00ZengNature PortfolioNature Communications2041-17232024-11-0115111410.1038/s41467-024-54172-7Unveiling RNA structure-mediated regulations of RNA stability in wheatHaidan Wu0Haopeng Yu1Yueying Zhang2Bibo Yang3Wenqing Sun4Lanying Ren5Yuchen Li6Qianqian Li7Bao Liu8Yiliang Ding9Huakun Zhang10Key Laboratory of Molecular Epigenetics of the Ministry of Education, Northeast Normal UniversityKey Laboratory of Molecular Epigenetics of the Ministry of Education, Northeast Normal UniversityKey Laboratory of Molecular Epigenetics of the Ministry of Education, Northeast Normal UniversityDepartment of Cell and Developmental Biology, John Innes Centre, Norwich Research ParkKey Laboratory of Molecular Epigenetics of the Ministry of Education, Northeast Normal UniversityKey Laboratory of Molecular Epigenetics of the Ministry of Education, Northeast Normal UniversityKey Laboratory of Molecular Epigenetics of the Ministry of Education, Northeast Normal UniversityDepartment of Cell and Developmental Biology, John Innes Centre, Norwich Research ParkKey Laboratory of Molecular Epigenetics of the Ministry of Education, Northeast Normal UniversityDepartment of Cell and Developmental Biology, John Innes Centre, Norwich Research ParkKey Laboratory of Molecular Epigenetics of the Ministry of Education, Northeast Normal UniversityAbstract Despite the critical role of mRNA stability in post-transcriptional gene regulation, research on this topic in wheat, a vital agricultural crop, remains unclear. Our study investigated the mRNA decay landscape of durum wheat (Triticum turgidum L. ssp. durum, BBAA), revealing subgenomic asymmetry in mRNA stability and its impact on steady-state mRNA abundance. Our findings indicate that the 3’ UTR structure and homoeolog preference for RNA structural motifs can influence mRNA stability, leading to subgenomic RNA decay imbalance. Furthermore, single-nucleotide variations (SNVs) selected for RNA structural motifs during domestication can cause variations in subgenomic mRNA stability and subsequent changes in steady-state expression levels. Our research on the transcriptome stability of polyploid wheat highlights the regulatory role of non-coding region structures in mRNA stability, and how domestication shaped RNA structure, altering subgenomic mRNA stability. These results illustrate the importance of RNA structure-mediated post-transcriptional gene regulation in wheat and pave the way for its potential use in crop improvement.https://doi.org/10.1038/s41467-024-54172-7
spellingShingle Haidan Wu
Haopeng Yu
Yueying Zhang
Bibo Yang
Wenqing Sun
Lanying Ren
Yuchen Li
Qianqian Li
Bao Liu
Yiliang Ding
Huakun Zhang
Unveiling RNA structure-mediated regulations of RNA stability in wheat
Nature Communications
title Unveiling RNA structure-mediated regulations of RNA stability in wheat
title_full Unveiling RNA structure-mediated regulations of RNA stability in wheat
title_fullStr Unveiling RNA structure-mediated regulations of RNA stability in wheat
title_full_unstemmed Unveiling RNA structure-mediated regulations of RNA stability in wheat
title_short Unveiling RNA structure-mediated regulations of RNA stability in wheat
title_sort unveiling rna structure mediated regulations of rna stability in wheat
url https://doi.org/10.1038/s41467-024-54172-7
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