Gingival fibroblast seeded bioengineered scaffolds for treatment of localized gingival recession
Gingival recession is a prevalent issue present in most of the Indian population, associated with interproximal tissue deficiency, leading to dental problems. Its treatment has remained a major problem in the field of periodontics due to autologous graft morbidity and limited healing associated with...
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Elsevier
2025-06-01
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| Series: | Biomedical Engineering Advances |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667099224000318 |
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| author | Rajul Chordia Aritri Ghosh Shalini Dasgupta Sayandeep Saha Tirthankar Debnath Ashit Kumar Pal Ananya Barui |
| author_facet | Rajul Chordia Aritri Ghosh Shalini Dasgupta Sayandeep Saha Tirthankar Debnath Ashit Kumar Pal Ananya Barui |
| author_sort | Rajul Chordia |
| collection | DOAJ |
| description | Gingival recession is a prevalent issue present in most of the Indian population, associated with interproximal tissue deficiency, leading to dental problems. Its treatment has remained a major problem in the field of periodontics due to autologous graft morbidity and limited healing associated with the current artificial grafts. The present study aims to is to develop bio-engineered chitosan-gelatin scaffolds seeded with primary gingival fibroblasts to address gingival recession as noninvasive grafts. Gingival fibroblasts were seeded on scaffolds with varying chitosan-gelatin ratios (1:1, 1:3) (v/v) and a chitosan control. Comprehensive characterization included morphological, mechanical, biochemical, and cellular analyses including cell viability, migration and transcriptomic studies. The chitosan-gelatin scaffolds (1:3) demonstrated a highly porous architecture with satisfactory biodegradation and swelling capacity. Furthermore, in vitro studies show significantly higher cellular compatibility, fibroblast migration, and F-actin expression. The upregulation of FGF-2 gene in this scaffold indicates its potential for promoting fibroblastic growth and improved wound healing potential. In addition, the antibacterial impact reflect its clinical potential of the fibroblast-seeded chitosan-gelatin (1:3) scaffold for potential tissue engineering applications in periodontal regeneration. |
| format | Article |
| id | doaj-art-4147d9131b7c4a04835b9cf57a12d7c2 |
| institution | Kabale University |
| issn | 2667-0992 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedical Engineering Advances |
| spelling | doaj-art-4147d9131b7c4a04835b9cf57a12d7c22025-08-20T03:45:28ZengElsevierBiomedical Engineering Advances2667-09922025-06-01910014210.1016/j.bea.2024.100142Gingival fibroblast seeded bioengineered scaffolds for treatment of localized gingival recessionRajul Chordia0Aritri Ghosh1Shalini Dasgupta2Sayandeep Saha3Tirthankar Debnath4Ashit Kumar Pal5Ananya Barui6Department of Periodontics, Dr. R. Ahmed Dental College and Hospital, 114, Acharya Jagadish Chandra Bose Road, Sealdah, Kolkata-700014, IndiaCentre for Healthcare Science and Technology, Indian Institute of Engineering, Science and Technology, Shibpur, Howrah-711103, IndiaCentre for Healthcare Science and Technology, Indian Institute of Engineering, Science and Technology, Shibpur, Howrah-711103, IndiaCentre for Healthcare Science and Technology, Indian Institute of Engineering, Science and Technology, Shibpur, Howrah-711103, IndiaDepartment of Periodontics, Dr. R. Ahmed Dental College and Hospital, 114, Acharya Jagadish Chandra Bose Road, Sealdah, Kolkata-700014, IndiaDepartment of Periodontics, Dr. R. Ahmed Dental College and Hospital, 114, Acharya Jagadish Chandra Bose Road, Sealdah, Kolkata-700014, IndiaCentre for Healthcare Science and Technology, Indian Institute of Engineering, Science and Technology, Shibpur, Howrah-711103, India; Corresponding author.Gingival recession is a prevalent issue present in most of the Indian population, associated with interproximal tissue deficiency, leading to dental problems. Its treatment has remained a major problem in the field of periodontics due to autologous graft morbidity and limited healing associated with the current artificial grafts. The present study aims to is to develop bio-engineered chitosan-gelatin scaffolds seeded with primary gingival fibroblasts to address gingival recession as noninvasive grafts. Gingival fibroblasts were seeded on scaffolds with varying chitosan-gelatin ratios (1:1, 1:3) (v/v) and a chitosan control. Comprehensive characterization included morphological, mechanical, biochemical, and cellular analyses including cell viability, migration and transcriptomic studies. The chitosan-gelatin scaffolds (1:3) demonstrated a highly porous architecture with satisfactory biodegradation and swelling capacity. Furthermore, in vitro studies show significantly higher cellular compatibility, fibroblast migration, and F-actin expression. The upregulation of FGF-2 gene in this scaffold indicates its potential for promoting fibroblastic growth and improved wound healing potential. In addition, the antibacterial impact reflect its clinical potential of the fibroblast-seeded chitosan-gelatin (1:3) scaffold for potential tissue engineering applications in periodontal regeneration.http://www.sciencedirect.com/science/article/pii/S2667099224000318Gingival recessionPrimary gingival fibroblastBioengineered scaffoldsAnti-microbialPeriodontal Treatments |
| spellingShingle | Rajul Chordia Aritri Ghosh Shalini Dasgupta Sayandeep Saha Tirthankar Debnath Ashit Kumar Pal Ananya Barui Gingival fibroblast seeded bioengineered scaffolds for treatment of localized gingival recession Biomedical Engineering Advances Gingival recession Primary gingival fibroblast Bioengineered scaffolds Anti-microbial Periodontal Treatments |
| title | Gingival fibroblast seeded bioengineered scaffolds for treatment of localized gingival recession |
| title_full | Gingival fibroblast seeded bioengineered scaffolds for treatment of localized gingival recession |
| title_fullStr | Gingival fibroblast seeded bioengineered scaffolds for treatment of localized gingival recession |
| title_full_unstemmed | Gingival fibroblast seeded bioengineered scaffolds for treatment of localized gingival recession |
| title_short | Gingival fibroblast seeded bioengineered scaffolds for treatment of localized gingival recession |
| title_sort | gingival fibroblast seeded bioengineered scaffolds for treatment of localized gingival recession |
| topic | Gingival recession Primary gingival fibroblast Bioengineered scaffolds Anti-microbial Periodontal Treatments |
| url | http://www.sciencedirect.com/science/article/pii/S2667099224000318 |
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