Heterogeneity as a feature: unraveling chromatin’s role in nuclear mechanics

Mechanical forces are a ubiquitous feature of the cellular environment. These forces propagate to the nucleus, where the mechanical response is critical for cellular function and survival. In addition to the nuclear lamina and cytoskeletal connections, chromatin is a key structural and mechanorespon...

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Main Authors: Wessel S. Rodenburg, Amy R. Strom, Jorine M. Eeftens
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:Nucleus
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Online Access:https://www.tandfonline.com/doi/10.1080/19491034.2025.2545037
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author Wessel S. Rodenburg
Amy R. Strom
Jorine M. Eeftens
author_facet Wessel S. Rodenburg
Amy R. Strom
Jorine M. Eeftens
author_sort Wessel S. Rodenburg
collection DOAJ
description Mechanical forces are a ubiquitous feature of the cellular environment. These forces propagate to the nucleus, where the mechanical response is critical for cellular function and survival. In addition to the nuclear lamina and cytoskeletal connections, chromatin is a key structural and mechanoresponsive element which not only contributes to bulk stiffness but also dynamically adapts its organization in response to mechanical stress. Crucially, chromatin is not a uniform material – its organization and mechanical properties vary across time, cell state, and even within individual nuclei. This heterogeneity underpins compartmentalization, gene regulation, and potentially, disease states when disrupted. In this review, we summarize recent experimental advances that have illuminated chromatin’s role in nuclear mechanics, emphasizing the importance of heterogeneity. We argue that an integrated, multiscale, and quantitative framework is essential for dissecting chromatin’s mechanical contributions. By doing so, the field will be better positioned to link nuclear mechanics to functional biological outcomes.
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publisher Taylor & Francis Group
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spelling doaj-art-414226fbd7ed49e2b13caf97e1472ff42025-08-21T10:55:22ZengTaylor & Francis GroupNucleus1949-10341949-10422025-12-0116110.1080/19491034.2025.2545037Heterogeneity as a feature: unraveling chromatin’s role in nuclear mechanicsWessel S. Rodenburg0Amy R. Strom1Jorine M. Eeftens2Institute for Molecules and Materials, Radboud University, Nijmegen, The NetherlandsDepartment of Chemical and Biological Engineering, Princeton University, Princeton, NJ, USAInstitute for Molecules and Materials, Radboud University, Nijmegen, The NetherlandsMechanical forces are a ubiquitous feature of the cellular environment. These forces propagate to the nucleus, where the mechanical response is critical for cellular function and survival. In addition to the nuclear lamina and cytoskeletal connections, chromatin is a key structural and mechanoresponsive element which not only contributes to bulk stiffness but also dynamically adapts its organization in response to mechanical stress. Crucially, chromatin is not a uniform material – its organization and mechanical properties vary across time, cell state, and even within individual nuclei. This heterogeneity underpins compartmentalization, gene regulation, and potentially, disease states when disrupted. In this review, we summarize recent experimental advances that have illuminated chromatin’s role in nuclear mechanics, emphasizing the importance of heterogeneity. We argue that an integrated, multiscale, and quantitative framework is essential for dissecting chromatin’s mechanical contributions. By doing so, the field will be better positioned to link nuclear mechanics to functional biological outcomes.https://www.tandfonline.com/doi/10.1080/19491034.2025.2545037Chromatingenome organizationheterogeneitymultiscale quantificationnuclear mechanics
spellingShingle Wessel S. Rodenburg
Amy R. Strom
Jorine M. Eeftens
Heterogeneity as a feature: unraveling chromatin’s role in nuclear mechanics
Nucleus
Chromatin
genome organization
heterogeneity
multiscale quantification
nuclear mechanics
title Heterogeneity as a feature: unraveling chromatin’s role in nuclear mechanics
title_full Heterogeneity as a feature: unraveling chromatin’s role in nuclear mechanics
title_fullStr Heterogeneity as a feature: unraveling chromatin’s role in nuclear mechanics
title_full_unstemmed Heterogeneity as a feature: unraveling chromatin’s role in nuclear mechanics
title_short Heterogeneity as a feature: unraveling chromatin’s role in nuclear mechanics
title_sort heterogeneity as a feature unraveling chromatin s role in nuclear mechanics
topic Chromatin
genome organization
heterogeneity
multiscale quantification
nuclear mechanics
url https://www.tandfonline.com/doi/10.1080/19491034.2025.2545037
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