Antibiotic Resistance of Enterobacteriaceae in Microbiomes Associated with Poultry Farming
Antibiotics have long been overused for non-therapeutic purposes. As a result, Escherichia, Enterobacter, Klebsiella, and Proteus in avian microbiomes have become reservoirs for genetic determinants of resistance, thus spreading resistance to antibiotics and contaminating raw materials and finished...
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| Format: | Article |
| Language: | English |
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Kemerovo State University
2023-12-01
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| Series: | Техника и технология пищевых производств |
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| Online Access: | https://fptt.ru/en/issues/22269/22222/ |
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| author | Anna S. Krivonogova Irina M. Donnik Albina G. Isaeva Egor A. Loginov Maxim V. Petropavlovskiy Elisey N. Bespamyatnykh |
| author_facet | Anna S. Krivonogova Irina M. Donnik Albina G. Isaeva Egor A. Loginov Maxim V. Petropavlovskiy Elisey N. Bespamyatnykh |
| author_sort | Anna S. Krivonogova |
| collection | DOAJ |
| description | Antibiotics have long been overused for non-therapeutic purposes. As a result, Escherichia, Enterobacter, Klebsiella, and Proteus in avian microbiomes have become reservoirs for genetic determinants of resistance, thus spreading resistance to antibiotics and contaminating raw materials and finished products. The food industry is looking for alternative means to preserve health and maintain high productivity of commercial poultry, e.g., probiotics, phytobiotics, organic acids, etc. The research featured the effect of antibiotics and phytobiotics on enteropathogenic bacteria in the microbiomes of broiler chicken. Escherichia coli bacteria were cultivated in vitro in subthreshold concentrations on nutrient media with antibiotics for 37 days to study the effect of low doses of antibiotics on the sensitivity of isolates. The study involved microbiocenoses of broiler chicken that received avilamycin A and a phytobiotic based on Brassica juncea, Linum usitatissimum, and Nigella sativa L. A set of experiments covered the species composition of opportunistic Enterobacteriaceae, the phenotypic sensitivity to antibiotics, and the genetic determinants of resistance, as well as the antimicrobial potential of phytobiotics. E. coli developed no resistance for 37 days when the antibiotic dose remained below minimal inhibitory. Opportunistic gram-negative Enterobacterales predominated in all litter samples. E. coli, Klebsiella pneumoniae, and Proteus mirabilis accounted for more than 30% of all isolated strains. Avilamycin A and the phytobiotic affected the coccal microflora but had no effect on the genus-species composition of Enterobacteriaceae. Litter samples from both experimental and control groups demonstrated K. pneumoniae with severe phenotypic resistance to ciprofloxacin, as well as blaDHA genes. In broiler farming, maintenance and circulation of resistance agents depends on litter microbiota. In this research, the chicken that received the phytobiotic showed the lowest level of resistance to ciprofloxacin while the groups that received avilamycin A had the highest resistance results. During a broiler’s life span, bacteria with no active resistance determinants in their genome remained sensitive to antibiotics, even though the contact with the latter was constant. Phytobiotics showed good prospects for broiler farming as food additive that could reduce and eventually eliminate the intake of antibiotics. |
| format | Article |
| id | doaj-art-41405f93db2145acb8a073833fd5728f |
| institution | Kabale University |
| issn | 2074-9414 2313-1748 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Kemerovo State University |
| record_format | Article |
| series | Техника и технология пищевых производств |
| spelling | doaj-art-41405f93db2145acb8a073833fd5728f2025-02-03T10:40:51ZengKemerovo State UniversityТехника и технология пищевых производств2074-94142313-17482023-12-0153471071710.21603/2074-9414-2023-4-2472Antibiotic Resistance of Enterobacteriaceae in Microbiomes Associated with Poultry FarmingAnna S. Krivonogova0https://orcid.org/0000-0003-1918-3030Irina M. Donnik1https://orcid.org/0000-0001-8349-3004Albina G. Isaeva2https://orcid.org/0000-0001-8395-1247Egor A. Loginov3https://orcid.org/0009-0007-0810-8365Maxim V. Petropavlovskiy4https://orcid.org/0000-0002-9892-6092Elisey N. Bespamyatnykh5https://orcid.org/0000-0003-4347-6554Ural State Agrarian University , Yekaterinburg, RussiaUral State Agrarian University , Yekaterinburg, RussiaUral State Agrarian University , Yekaterinburg, RussiaUral State Agrarian University , Yekaterinburg, RussiaUral State Agrarian University , Yekaterinburg, RussiaUral State Agrarian University , Yekaterinburg, RussiaAntibiotics have long been overused for non-therapeutic purposes. As a result, Escherichia, Enterobacter, Klebsiella, and Proteus in avian microbiomes have become reservoirs for genetic determinants of resistance, thus spreading resistance to antibiotics and contaminating raw materials and finished products. The food industry is looking for alternative means to preserve health and maintain high productivity of commercial poultry, e.g., probiotics, phytobiotics, organic acids, etc. The research featured the effect of antibiotics and phytobiotics on enteropathogenic bacteria in the microbiomes of broiler chicken. Escherichia coli bacteria were cultivated in vitro in subthreshold concentrations on nutrient media with antibiotics for 37 days to study the effect of low doses of antibiotics on the sensitivity of isolates. The study involved microbiocenoses of broiler chicken that received avilamycin A and a phytobiotic based on Brassica juncea, Linum usitatissimum, and Nigella sativa L. A set of experiments covered the species composition of opportunistic Enterobacteriaceae, the phenotypic sensitivity to antibiotics, and the genetic determinants of resistance, as well as the antimicrobial potential of phytobiotics. E. coli developed no resistance for 37 days when the antibiotic dose remained below minimal inhibitory. Opportunistic gram-negative Enterobacterales predominated in all litter samples. E. coli, Klebsiella pneumoniae, and Proteus mirabilis accounted for more than 30% of all isolated strains. Avilamycin A and the phytobiotic affected the coccal microflora but had no effect on the genus-species composition of Enterobacteriaceae. Litter samples from both experimental and control groups demonstrated K. pneumoniae with severe phenotypic resistance to ciprofloxacin, as well as blaDHA genes. In broiler farming, maintenance and circulation of resistance agents depends on litter microbiota. In this research, the chicken that received the phytobiotic showed the lowest level of resistance to ciprofloxacin while the groups that received avilamycin A had the highest resistance results. During a broiler’s life span, bacteria with no active resistance determinants in their genome remained sensitive to antibiotics, even though the contact with the latter was constant. Phytobiotics showed good prospects for broiler farming as food additive that could reduce and eventually eliminate the intake of antibiotics.https://fptt.ru/en/issues/22269/22222/antibiotic resistanceenterobacteriaantibioticphytobioticmicrobiomebroilers |
| spellingShingle | Anna S. Krivonogova Irina M. Donnik Albina G. Isaeva Egor A. Loginov Maxim V. Petropavlovskiy Elisey N. Bespamyatnykh Antibiotic Resistance of Enterobacteriaceae in Microbiomes Associated with Poultry Farming Техника и технология пищевых производств antibiotic resistance enterobacteria antibiotic phytobiotic microbiome broilers |
| title | Antibiotic Resistance of Enterobacteriaceae in Microbiomes Associated with Poultry Farming |
| title_full | Antibiotic Resistance of Enterobacteriaceae in Microbiomes Associated with Poultry Farming |
| title_fullStr | Antibiotic Resistance of Enterobacteriaceae in Microbiomes Associated with Poultry Farming |
| title_full_unstemmed | Antibiotic Resistance of Enterobacteriaceae in Microbiomes Associated with Poultry Farming |
| title_short | Antibiotic Resistance of Enterobacteriaceae in Microbiomes Associated with Poultry Farming |
| title_sort | antibiotic resistance of enterobacteriaceae in microbiomes associated with poultry farming |
| topic | antibiotic resistance enterobacteria antibiotic phytobiotic microbiome broilers |
| url | https://fptt.ru/en/issues/22269/22222/ |
| work_keys_str_mv | AT annaskrivonogova antibioticresistanceofenterobacteriaceaeinmicrobiomesassociatedwithpoultryfarming AT irinamdonnik antibioticresistanceofenterobacteriaceaeinmicrobiomesassociatedwithpoultryfarming AT albinagisaeva antibioticresistanceofenterobacteriaceaeinmicrobiomesassociatedwithpoultryfarming AT egoraloginov antibioticresistanceofenterobacteriaceaeinmicrobiomesassociatedwithpoultryfarming AT maximvpetropavlovskiy antibioticresistanceofenterobacteriaceaeinmicrobiomesassociatedwithpoultryfarming AT eliseynbespamyatnykh antibioticresistanceofenterobacteriaceaeinmicrobiomesassociatedwithpoultryfarming |