Effect of the Comonomer Nature on the Cytotoxicity and Mechanical Properties of a Cryogel Based on Sodium 2-Acrylamido-2-methyl-1-propanesulfonate Copolymers
Currently, for the treatment of corneal diseases (keratitis–corneal opacities), synthetic corneal analogs based on polymer films or hydrogels are being developed. The requirements for the material include biocompatibility, the presence of a developed system of macropores, transparency, rapid swellin...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-05-01
|
| Series: | Colloids and Interfaces |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2504-5377/9/3/34 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850156373185134592 |
|---|---|
| author | Olga Iakobson Elena Ivan’kova Yulia Nashchekina Gleb Vaganov Svetlana Laishevkina Natalia Shevchenko |
| author_facet | Olga Iakobson Elena Ivan’kova Yulia Nashchekina Gleb Vaganov Svetlana Laishevkina Natalia Shevchenko |
| author_sort | Olga Iakobson |
| collection | DOAJ |
| description | Currently, for the treatment of corneal diseases (keratitis–corneal opacities), synthetic corneal analogs based on polymer films or hydrogels are being developed. The requirements for the material include biocompatibility, the presence of a developed system of macropores, transparency, rapid swelling, and mechanical strength. Here, with the aim of preparing such materials, a series of gels based on a copolymer of 2-acrylamido-2-methyl-1-propanesulfonic acid (AMP) and 2-hydroxyethyl methacrylate (or vinyl acetate, or ethyl acrylate) were obtained using cryotropic gelation. It was shown that transparent cryogels can be obtained based on the sulfonate-containing comonomer 2-acrylamido-2-methyl-1-propanesulfonic acid at a crosslinking agent concentration of 2.2 mol.%, while the nature of the acrylate comonomer did not show any effect on transparency. It was found that when using AMP and ethyl acrylate, cryogels with a developed system of macropores with a diameter of 50 to 250 μm were formed, and the mechanical strength of such cryogels was sufficient for their subsequent use as corneal implants. Moreover, the PAMP hydrogel containing 2-hydroxyethyl methacrylate or ethyl acrylate units did not affect the viability of cells even after 1 month. |
| format | Article |
| id | doaj-art-412fb8623a9440f0942683bf6d52e102 |
| institution | OA Journals |
| issn | 2504-5377 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Colloids and Interfaces |
| spelling | doaj-art-412fb8623a9440f0942683bf6d52e1022025-08-20T02:24:34ZengMDPI AGColloids and Interfaces2504-53772025-05-01933410.3390/colloids9030034Effect of the Comonomer Nature on the Cytotoxicity and Mechanical Properties of a Cryogel Based on Sodium 2-Acrylamido-2-methyl-1-propanesulfonate CopolymersOlga Iakobson0Elena Ivan’kova1Yulia Nashchekina2Gleb Vaganov3Svetlana Laishevkina4Natalia Shevchenko5Branch of Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute»—Institute of Macromolecular Compounds, 199004 Saint-Petersburg, RussiaBranch of Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute»—Institute of Macromolecular Compounds, 199004 Saint-Petersburg, RussiaInstitute of Cytology, Russian Academy of Sciences, 194064 Saint-Petersburg, RussiaBranch of Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute»—Institute of Macromolecular Compounds, 199004 Saint-Petersburg, RussiaBranch of Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute»—Institute of Macromolecular Compounds, 199004 Saint-Petersburg, RussiaBranch of Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute»—Institute of Macromolecular Compounds, 199004 Saint-Petersburg, RussiaCurrently, for the treatment of corneal diseases (keratitis–corneal opacities), synthetic corneal analogs based on polymer films or hydrogels are being developed. The requirements for the material include biocompatibility, the presence of a developed system of macropores, transparency, rapid swelling, and mechanical strength. Here, with the aim of preparing such materials, a series of gels based on a copolymer of 2-acrylamido-2-methyl-1-propanesulfonic acid (AMP) and 2-hydroxyethyl methacrylate (or vinyl acetate, or ethyl acrylate) were obtained using cryotropic gelation. It was shown that transparent cryogels can be obtained based on the sulfonate-containing comonomer 2-acrylamido-2-methyl-1-propanesulfonic acid at a crosslinking agent concentration of 2.2 mol.%, while the nature of the acrylate comonomer did not show any effect on transparency. It was found that when using AMP and ethyl acrylate, cryogels with a developed system of macropores with a diameter of 50 to 250 μm were formed, and the mechanical strength of such cryogels was sufficient for their subsequent use as corneal implants. Moreover, the PAMP hydrogel containing 2-hydroxyethyl methacrylate or ethyl acrylate units did not affect the viability of cells even after 1 month.https://www.mdpi.com/2504-5377/9/3/34polymer cryogelpolyelectrolytecytotoxicitymechanical properties |
| spellingShingle | Olga Iakobson Elena Ivan’kova Yulia Nashchekina Gleb Vaganov Svetlana Laishevkina Natalia Shevchenko Effect of the Comonomer Nature on the Cytotoxicity and Mechanical Properties of a Cryogel Based on Sodium 2-Acrylamido-2-methyl-1-propanesulfonate Copolymers Colloids and Interfaces polymer cryogel polyelectrolyte cytotoxicity mechanical properties |
| title | Effect of the Comonomer Nature on the Cytotoxicity and Mechanical Properties of a Cryogel Based on Sodium 2-Acrylamido-2-methyl-1-propanesulfonate Copolymers |
| title_full | Effect of the Comonomer Nature on the Cytotoxicity and Mechanical Properties of a Cryogel Based on Sodium 2-Acrylamido-2-methyl-1-propanesulfonate Copolymers |
| title_fullStr | Effect of the Comonomer Nature on the Cytotoxicity and Mechanical Properties of a Cryogel Based on Sodium 2-Acrylamido-2-methyl-1-propanesulfonate Copolymers |
| title_full_unstemmed | Effect of the Comonomer Nature on the Cytotoxicity and Mechanical Properties of a Cryogel Based on Sodium 2-Acrylamido-2-methyl-1-propanesulfonate Copolymers |
| title_short | Effect of the Comonomer Nature on the Cytotoxicity and Mechanical Properties of a Cryogel Based on Sodium 2-Acrylamido-2-methyl-1-propanesulfonate Copolymers |
| title_sort | effect of the comonomer nature on the cytotoxicity and mechanical properties of a cryogel based on sodium 2 acrylamido 2 methyl 1 propanesulfonate copolymers |
| topic | polymer cryogel polyelectrolyte cytotoxicity mechanical properties |
| url | https://www.mdpi.com/2504-5377/9/3/34 |
| work_keys_str_mv | AT olgaiakobson effectofthecomonomernatureonthecytotoxicityandmechanicalpropertiesofacryogelbasedonsodium2acrylamido2methyl1propanesulfonatecopolymers AT elenaivankova effectofthecomonomernatureonthecytotoxicityandmechanicalpropertiesofacryogelbasedonsodium2acrylamido2methyl1propanesulfonatecopolymers AT yulianashchekina effectofthecomonomernatureonthecytotoxicityandmechanicalpropertiesofacryogelbasedonsodium2acrylamido2methyl1propanesulfonatecopolymers AT glebvaganov effectofthecomonomernatureonthecytotoxicityandmechanicalpropertiesofacryogelbasedonsodium2acrylamido2methyl1propanesulfonatecopolymers AT svetlanalaishevkina effectofthecomonomernatureonthecytotoxicityandmechanicalpropertiesofacryogelbasedonsodium2acrylamido2methyl1propanesulfonatecopolymers AT nataliashevchenko effectofthecomonomernatureonthecytotoxicityandmechanicalpropertiesofacryogelbasedonsodium2acrylamido2methyl1propanesulfonatecopolymers |