Junshanyinzhen tea extract prevents obesity by regulating gut microbiota and metabolic endotoxemia in high-fat diet fed rats
Obesity is associated with gut dysbiosis and metabolic endotoxin. Junshanyinzhen tea extract (JSTE) reduced fat accumulation and body weight in obese mice. However, the effects and mechanism of JSTE in preventing obesity were unclear. Therefore, we used different doses of JSTE (75, 150 and 300 mg/(k...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tsinghua University Press
2024-07-01
|
| Series: | Food Science and Human Wellness |
| Subjects: | |
| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250169 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841550177624653824 |
|---|---|
| author | Jian Ouyang Xiuping Li Changwei Liu Danmin Lu Jie Ouyang Fang Zhou Qi Liu Jianan Huang Zhonghua Liu |
| author_facet | Jian Ouyang Xiuping Li Changwei Liu Danmin Lu Jie Ouyang Fang Zhou Qi Liu Jianan Huang Zhonghua Liu |
| author_sort | Jian Ouyang |
| collection | DOAJ |
| description | Obesity is associated with gut dysbiosis and metabolic endotoxin. Junshanyinzhen tea extract (JSTE) reduced fat accumulation and body weight in obese mice. However, the effects and mechanism of JSTE in preventing obesity were unclear. Therefore, we used different doses of JSTE (75, 150 and 300 mg/(kg·day)) to evaluate the effect on high-fat diet (HFD) -induced rats under 8 weeks of intervention. Here, our results showed that JSTE could significantly reduce body weight gain, blood lipid levels and fat accumulation, improve fatty damage in liver tissue (P < 0.05). In addition, JSTE increased the expression of intestinal tight junction proteins (P < 0.05), relieved metabolic endotoxemia (P < 0.05) and chronic low- grade inflammation in HFD rats. Sequencing of fecal samples showed that JSTE could effectively reverse the microbial diversity and the ratio of Firmicutes to Bacteroidetes to normal levels in HFD- fed rats. Desulfovibrioceae and Erysipelotrichaceae, which are positively related to obesity, were decreased by JSTE intervention (P < 0.05). while Bifidobacteriaceae, Bacteroidaceae, Akkermansia, and Clostridium, which are negatively related to obesity, were increased. Together, these results suggested that JSTE might effectively prevent obesity by modulating gut microbiota dysbiosis, intestinal barrier dysfunction, metabolic endotoxemia and chronic low-grade inflammation in HFD-induced rats. |
| format | Article |
| id | doaj-art-412b7c9d759c4dd99a9c64040e306afc |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-412b7c9d759c4dd99a9c64040e306afc2025-01-10T06:56:48ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-07-011342036204710.26599/FSHW.2022.9250169Junshanyinzhen tea extract prevents obesity by regulating gut microbiota and metabolic endotoxemia in high-fat diet fed ratsJian Ouyang0Xiuping Li1Changwei Liu2Danmin Lu3Jie Ouyang4Fang Zhou5Qi Liu6Jianan Huang7Zhonghua Liu8Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, ChinaKey Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, ChinaKey Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, ChinaKey Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, ChinaKey Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, ChinaKey Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, ChinaKey Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, ChinaKey Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, ChinaKey Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, ChinaObesity is associated with gut dysbiosis and metabolic endotoxin. Junshanyinzhen tea extract (JSTE) reduced fat accumulation and body weight in obese mice. However, the effects and mechanism of JSTE in preventing obesity were unclear. Therefore, we used different doses of JSTE (75, 150 and 300 mg/(kg·day)) to evaluate the effect on high-fat diet (HFD) -induced rats under 8 weeks of intervention. Here, our results showed that JSTE could significantly reduce body weight gain, blood lipid levels and fat accumulation, improve fatty damage in liver tissue (P < 0.05). In addition, JSTE increased the expression of intestinal tight junction proteins (P < 0.05), relieved metabolic endotoxemia (P < 0.05) and chronic low- grade inflammation in HFD rats. Sequencing of fecal samples showed that JSTE could effectively reverse the microbial diversity and the ratio of Firmicutes to Bacteroidetes to normal levels in HFD- fed rats. Desulfovibrioceae and Erysipelotrichaceae, which are positively related to obesity, were decreased by JSTE intervention (P < 0.05). while Bifidobacteriaceae, Bacteroidaceae, Akkermansia, and Clostridium, which are negatively related to obesity, were increased. Together, these results suggested that JSTE might effectively prevent obesity by modulating gut microbiota dysbiosis, intestinal barrier dysfunction, metabolic endotoxemia and chronic low-grade inflammation in HFD-induced rats.https://www.sciopen.com/article/10.26599/FSHW.2022.9250169junshanyinzhen teaobesitygut microbiotagut barrier functionmetabolic endotoxemia |
| spellingShingle | Jian Ouyang Xiuping Li Changwei Liu Danmin Lu Jie Ouyang Fang Zhou Qi Liu Jianan Huang Zhonghua Liu Junshanyinzhen tea extract prevents obesity by regulating gut microbiota and metabolic endotoxemia in high-fat diet fed rats Food Science and Human Wellness junshanyinzhen tea obesity gut microbiota gut barrier function metabolic endotoxemia |
| title | Junshanyinzhen tea extract prevents obesity by regulating gut microbiota and metabolic endotoxemia in high-fat diet fed rats |
| title_full | Junshanyinzhen tea extract prevents obesity by regulating gut microbiota and metabolic endotoxemia in high-fat diet fed rats |
| title_fullStr | Junshanyinzhen tea extract prevents obesity by regulating gut microbiota and metabolic endotoxemia in high-fat diet fed rats |
| title_full_unstemmed | Junshanyinzhen tea extract prevents obesity by regulating gut microbiota and metabolic endotoxemia in high-fat diet fed rats |
| title_short | Junshanyinzhen tea extract prevents obesity by regulating gut microbiota and metabolic endotoxemia in high-fat diet fed rats |
| title_sort | junshanyinzhen tea extract prevents obesity by regulating gut microbiota and metabolic endotoxemia in high fat diet fed rats |
| topic | junshanyinzhen tea obesity gut microbiota gut barrier function metabolic endotoxemia |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250169 |
| work_keys_str_mv | AT jianouyang junshanyinzhenteaextractpreventsobesitybyregulatinggutmicrobiotaandmetabolicendotoxemiainhighfatdietfedrats AT xiupingli junshanyinzhenteaextractpreventsobesitybyregulatinggutmicrobiotaandmetabolicendotoxemiainhighfatdietfedrats AT changweiliu junshanyinzhenteaextractpreventsobesitybyregulatinggutmicrobiotaandmetabolicendotoxemiainhighfatdietfedrats AT danminlu junshanyinzhenteaextractpreventsobesitybyregulatinggutmicrobiotaandmetabolicendotoxemiainhighfatdietfedrats AT jieouyang junshanyinzhenteaextractpreventsobesitybyregulatinggutmicrobiotaandmetabolicendotoxemiainhighfatdietfedrats AT fangzhou junshanyinzhenteaextractpreventsobesitybyregulatinggutmicrobiotaandmetabolicendotoxemiainhighfatdietfedrats AT qiliu junshanyinzhenteaextractpreventsobesitybyregulatinggutmicrobiotaandmetabolicendotoxemiainhighfatdietfedrats AT jiananhuang junshanyinzhenteaextractpreventsobesitybyregulatinggutmicrobiotaandmetabolicendotoxemiainhighfatdietfedrats AT zhonghualiu junshanyinzhenteaextractpreventsobesitybyregulatinggutmicrobiotaandmetabolicendotoxemiainhighfatdietfedrats |