Effects of Sr/F-bioactive glass nanoparticles on pH, elemental release, dentin remineralisation, and cytotoxicity of 1.1% NaF toothpaste
Objective: This study examined the effect of Sr/F-bioactive glass nanoparticles (Sr/F-BAG) concentration on 1.1% NaF toothpaste. The effects of additives on pH, fluoride and elemental release, dentin remineralisation, and cytotoxicity were determined. Materials and methods: Sr/F-BAG particles wer...
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Medical Journals Sweden
2025-07-01
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| Series: | Biomaterial Investigations in Dentistry |
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| Online Access: | https://medicaljournalssweden.se/biid/article/view/44239 |
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| author | Chananya Gesprasert Matana Kedtratad Parichart Naruphontjirakul Piyaphong Panpisut |
| author_facet | Chananya Gesprasert Matana Kedtratad Parichart Naruphontjirakul Piyaphong Panpisut |
| author_sort | Chananya Gesprasert |
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Objective: This study examined the effect of Sr/F-bioactive glass nanoparticles (Sr/F-BAG) concentration on 1.1% NaF toothpaste. The effects of additives on pH, fluoride and elemental release, dentin remineralisation, and cytotoxicity were determined.
Materials and methods: Sr/F-BAG particles were incorporated into 1.1% NaF toothpaste (0, 1, 2, and 4 wt%). F release and pH upon immersion in deionised water were determined using a fluoride-specific electrode and pH meter (n = 8). Elemental release was analysed using Inductively Coupled Plasma Optical Emission Spectroscopy (n = 3). Dentin remineralisation (mineral-to-collagen ratio) after application of experimental toothpaste was compared using Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR, n = 9). Cytotoxicity was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (n = 3). Colgate PreviDent 5000 Plus toothpaste (PV) was used as a commercial comparison.
Results: The addition of 0 to 4 wt% Sr/F-BAG linearly increased pH and F release of the 1.1% NaF toothpaste. Each 1 wt% increase in Sr/F-BAG concentration, raised pH by 0.3 and fluoride release by 457 ppm. The additives also enhanced the release of Ca, P, and Sr from the experimental toothpaste. At high concentration of Sr/F-BAG (4 wt%), the pH of the experimental toothpaste was comparable to PV (p > 0.05) but with significantly higher fluoride release (p < 0.05). However, PV demonstrated a significantly higher increase in mineral-to-collagen ratio compared to the experimental materials. The dentin surface treated with PV also showed more evident mineral precipitation. Furthermore, the experimental toothpaste containing 4 wt% Sr/F-BAG demonstrated higher cell viability (90%) than PV (56%).
Conclusion: The addition of Sr/F-BAG enhanced the release of F, Ca, P, Sr, and increased the pH of the toothpaste. However, the experimental toothpaste with added bioactive glass up to 4 wt% did not demonstrate superior remineralising effects compared to commercial 1.1% NaF toothpaste. In addition, the incorporation of Sr/F-BAG promoted the cytocompatibility of the experimental toothpaste.
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| format | Article |
| id | doaj-art-4124c53cc46c4198a85c3e12efbdb8ac |
| institution | Kabale University |
| issn | 2641-5275 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Medical Journals Sweden |
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| series | Biomaterial Investigations in Dentistry |
| spelling | doaj-art-4124c53cc46c4198a85c3e12efbdb8ac2025-08-20T03:30:01ZengMedical Journals SwedenBiomaterial Investigations in Dentistry2641-52752025-07-0112110.2340/biid.v12.44239Effects of Sr/F-bioactive glass nanoparticles on pH, elemental release, dentin remineralisation, and cytotoxicity of 1.1% NaF toothpasteChananya Gesprasert0Matana Kedtratad1Parichart Naruphontjirakul2Piyaphong Panpisut3Dental Department, Prasat Hospital, Surin, Thailand; Faculty of Dentistry, Thammasat University, Pathum Thani, ThailandFaculty of Dentistry, Thammasat University, Pathum Thani, ThailandBiological Engineering Program, Faculty of Engineering, King Mongkut’s University of Technology Thonburi, Bangkok, ThailandFaculty of Dentistry, Thammasat University, Pathum Thani, Thailand; Thammasat University Research Unit in Dental and Bone Substitute Biomaterials, Thammasat University, Pathum Thani, Thailand Objective: This study examined the effect of Sr/F-bioactive glass nanoparticles (Sr/F-BAG) concentration on 1.1% NaF toothpaste. The effects of additives on pH, fluoride and elemental release, dentin remineralisation, and cytotoxicity were determined. Materials and methods: Sr/F-BAG particles were incorporated into 1.1% NaF toothpaste (0, 1, 2, and 4 wt%). F release and pH upon immersion in deionised water were determined using a fluoride-specific electrode and pH meter (n = 8). Elemental release was analysed using Inductively Coupled Plasma Optical Emission Spectroscopy (n = 3). Dentin remineralisation (mineral-to-collagen ratio) after application of experimental toothpaste was compared using Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR, n = 9). Cytotoxicity was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (n = 3). Colgate PreviDent 5000 Plus toothpaste (PV) was used as a commercial comparison. Results: The addition of 0 to 4 wt% Sr/F-BAG linearly increased pH and F release of the 1.1% NaF toothpaste. Each 1 wt% increase in Sr/F-BAG concentration, raised pH by 0.3 and fluoride release by 457 ppm. The additives also enhanced the release of Ca, P, and Sr from the experimental toothpaste. At high concentration of Sr/F-BAG (4 wt%), the pH of the experimental toothpaste was comparable to PV (p > 0.05) but with significantly higher fluoride release (p < 0.05). However, PV demonstrated a significantly higher increase in mineral-to-collagen ratio compared to the experimental materials. The dentin surface treated with PV also showed more evident mineral precipitation. Furthermore, the experimental toothpaste containing 4 wt% Sr/F-BAG demonstrated higher cell viability (90%) than PV (56%). Conclusion: The addition of Sr/F-BAG enhanced the release of F, Ca, P, Sr, and increased the pH of the toothpaste. However, the experimental toothpaste with added bioactive glass up to 4 wt% did not demonstrate superior remineralising effects compared to commercial 1.1% NaF toothpaste. In addition, the incorporation of Sr/F-BAG promoted the cytocompatibility of the experimental toothpaste. https://medicaljournalssweden.se/biid/article/view/44239Bioactive glass5000 ppm toothpastefluoride releaseelemental releasecytotoxicityremineralisation |
| spellingShingle | Chananya Gesprasert Matana Kedtratad Parichart Naruphontjirakul Piyaphong Panpisut Effects of Sr/F-bioactive glass nanoparticles on pH, elemental release, dentin remineralisation, and cytotoxicity of 1.1% NaF toothpaste Biomaterial Investigations in Dentistry Bioactive glass 5000 ppm toothpaste fluoride release elemental release cytotoxicity remineralisation |
| title | Effects of Sr/F-bioactive glass nanoparticles on pH, elemental release, dentin remineralisation, and cytotoxicity of 1.1% NaF toothpaste |
| title_full | Effects of Sr/F-bioactive glass nanoparticles on pH, elemental release, dentin remineralisation, and cytotoxicity of 1.1% NaF toothpaste |
| title_fullStr | Effects of Sr/F-bioactive glass nanoparticles on pH, elemental release, dentin remineralisation, and cytotoxicity of 1.1% NaF toothpaste |
| title_full_unstemmed | Effects of Sr/F-bioactive glass nanoparticles on pH, elemental release, dentin remineralisation, and cytotoxicity of 1.1% NaF toothpaste |
| title_short | Effects of Sr/F-bioactive glass nanoparticles on pH, elemental release, dentin remineralisation, and cytotoxicity of 1.1% NaF toothpaste |
| title_sort | effects of sr f bioactive glass nanoparticles on ph elemental release dentin remineralisation and cytotoxicity of 1 1 naf toothpaste |
| topic | Bioactive glass 5000 ppm toothpaste fluoride release elemental release cytotoxicity remineralisation |
| url | https://medicaljournalssweden.se/biid/article/view/44239 |
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