Identification of the Insecticidal Effect of Cry Protein on a Variety of Potato Pests
【Objective】The study was conducted to explore the insecticidal effects of Cry protein on Leptinotarsa decemlineata, Spodoptera frugiperda, and Henosepilachna vigintioctopunctata, aiming to provide a foundation for the development of Cry transgenic potato plants with insect resistance.【Method】'X...
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Guangdong Academy of Agricultural Sciences
2024-10-01
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| Series: | Guangdong nongye kexue |
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| Online Access: | http://gdnykx.cnjournals.org/gdnykx/ch/reader/view_abstract.aspx?file_no=202410006 |
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| author | Deshan ZHOU Doudou YANG Juan LIU Weichao LIN Jie CHENG Suhua LI |
| author_facet | Deshan ZHOU Doudou YANG Juan LIU Weichao LIN Jie CHENG Suhua LI |
| author_sort | Deshan ZHOU |
| collection | DOAJ |
| description | 【Objective】The study was conducted to explore the insecticidal effects of Cry protein on Leptinotarsa decemlineata, Spodoptera frugiperda, and Henosepilachna vigintioctopunctata, aiming to provide a foundation for the development of Cry transgenic potato plants with insect resistance.【Method】'Xisen 6' and 'Favorita 15' potatoes were taken as the test materials and the aforementioned three pest insects as the test subjects. The Cry proteins (Cry1B, Cry1Ac, Cry3A, Cry3B) was applied by using both feed addition and leaf coating methods, and sterile water (CK) and different concentrations of Cry protein treatment groups were set up. The insecticidal effects of the Cry protein on the three insects were identified by comparing the larval mortality rate and biomass among different treatments.【Result】Cry3A protein at concentrations of 10, 20, and 100 μg/g showed significant effects in inhibiting the biomass and increasing the mortality rate of 2nd instar L. decemlineata. In contrast, under the same concentration, there was no significant difference in the biomass of 2nd instar L. decemlineata treated with Cry1B protein compared with the CK, indicating that Cry1B protein had no insecticidal effect on these larvae. Under the treatments with 100 μg/g of Cry1Ac and Cry1B proteins to feed S. frugiperda respectively, the results showed that Cry1Ac protein significantly inhibited the growth of S. frugiperda larvae, while there was no significant difference in larval biomass between the treatments with Cry1B and CK, indicating that Cry1B protein had no insecticidal effect on S. frugiperda. Treatment of 2nd instar H. vigintioctopunctata with 100 μg/g of Cry1B protein exhibited significant effects in inhibiting larval biomass and increasing mortality rate, with the mortality rates on the second day and third day being 50% and 100%, respectively. The results indicated that Cry1B protein had strong insecticidal effect on H. vigintioctopunctata.【Conclusion】Cry1Ac protein has a insecticidal effect on S. frugiperda, Cry3A protein exhibits insecticidal effect on L. decemlineata, and Cry1B protein demonstrates significant insecticidal effect on H. vigintioctopunctata, but it shows no insecticidal effect on L. decemlineata or S. frugiperda. |
| format | Article |
| id | doaj-art-41215e0d323b4ed4b1720b7740838827 |
| institution | Kabale University |
| issn | 1004-874X |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Guangdong Academy of Agricultural Sciences |
| record_format | Article |
| series | Guangdong nongye kexue |
| spelling | doaj-art-41215e0d323b4ed4b1720b77408388272025-01-04T07:39:37ZengGuangdong Academy of Agricultural SciencesGuangdong nongye kexue1004-874X2024-10-015110626910.16768/j.issn.1004-874X.2024.10.006202410006Identification of the Insecticidal Effect of Cry Protein on a Variety of Potato PestsDeshan ZHOU0Doudou YANG1Juan LIU2Weichao LIN3Jie CHENG4Suhua LI5College of Agronomy, Qingdao Agricultural University, Qingdao 266109, ChinaShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture/Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs/Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518000, ChinaShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture/Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs/Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518000, ChinaShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture/Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs/Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518000, ChinaShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture/Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs/Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518000, ChinaShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture/Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs/Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518000, China【Objective】The study was conducted to explore the insecticidal effects of Cry protein on Leptinotarsa decemlineata, Spodoptera frugiperda, and Henosepilachna vigintioctopunctata, aiming to provide a foundation for the development of Cry transgenic potato plants with insect resistance.【Method】'Xisen 6' and 'Favorita 15' potatoes were taken as the test materials and the aforementioned three pest insects as the test subjects. The Cry proteins (Cry1B, Cry1Ac, Cry3A, Cry3B) was applied by using both feed addition and leaf coating methods, and sterile water (CK) and different concentrations of Cry protein treatment groups were set up. The insecticidal effects of the Cry protein on the three insects were identified by comparing the larval mortality rate and biomass among different treatments.【Result】Cry3A protein at concentrations of 10, 20, and 100 μg/g showed significant effects in inhibiting the biomass and increasing the mortality rate of 2nd instar L. decemlineata. In contrast, under the same concentration, there was no significant difference in the biomass of 2nd instar L. decemlineata treated with Cry1B protein compared with the CK, indicating that Cry1B protein had no insecticidal effect on these larvae. Under the treatments with 100 μg/g of Cry1Ac and Cry1B proteins to feed S. frugiperda respectively, the results showed that Cry1Ac protein significantly inhibited the growth of S. frugiperda larvae, while there was no significant difference in larval biomass between the treatments with Cry1B and CK, indicating that Cry1B protein had no insecticidal effect on S. frugiperda. Treatment of 2nd instar H. vigintioctopunctata with 100 μg/g of Cry1B protein exhibited significant effects in inhibiting larval biomass and increasing mortality rate, with the mortality rates on the second day and third day being 50% and 100%, respectively. The results indicated that Cry1B protein had strong insecticidal effect on H. vigintioctopunctata.【Conclusion】Cry1Ac protein has a insecticidal effect on S. frugiperda, Cry3A protein exhibits insecticidal effect on L. decemlineata, and Cry1B protein demonstrates significant insecticidal effect on H. vigintioctopunctata, but it shows no insecticidal effect on L. decemlineata or S. frugiperda.http://gdnykx.cnjournals.org/gdnykx/ch/reader/view_abstract.aspx?file_no=202410006cry1b proteinleptinotarsa decemlineataspodoptera frugiperdahenosepilachna vigintioctopunctatainsecticidal effectbioassay |
| spellingShingle | Deshan ZHOU Doudou YANG Juan LIU Weichao LIN Jie CHENG Suhua LI Identification of the Insecticidal Effect of Cry Protein on a Variety of Potato Pests Guangdong nongye kexue cry1b protein leptinotarsa decemlineata spodoptera frugiperda henosepilachna vigintioctopunctata insecticidal effect bioassay |
| title | Identification of the Insecticidal Effect of Cry Protein on a Variety of Potato Pests |
| title_full | Identification of the Insecticidal Effect of Cry Protein on a Variety of Potato Pests |
| title_fullStr | Identification of the Insecticidal Effect of Cry Protein on a Variety of Potato Pests |
| title_full_unstemmed | Identification of the Insecticidal Effect of Cry Protein on a Variety of Potato Pests |
| title_short | Identification of the Insecticidal Effect of Cry Protein on a Variety of Potato Pests |
| title_sort | identification of the insecticidal effect of cry protein on a variety of potato pests |
| topic | cry1b protein leptinotarsa decemlineata spodoptera frugiperda henosepilachna vigintioctopunctata insecticidal effect bioassay |
| url | http://gdnykx.cnjournals.org/gdnykx/ch/reader/view_abstract.aspx?file_no=202410006 |
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