Genome‐wide analysis of HD‐Zip genes in Sophora alopecuroides and their role in salt stress response
Abstract We aimed to identify HD‐Zip (homologous domain leucine zipper) family genes based on the complete Sophora alopecuroides genome sequence. Eighty‐six Sophora alopecuroides HD‐Zip family (SaHDZ) genes were identified and categorized into four subclasses using phylogenetic analysis. Chromosome...
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| Format: | Article |
| Language: | English |
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Wiley
2024-09-01
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| Series: | The Plant Genome |
| Online Access: | https://doi.org/10.1002/tpg2.20504 |
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| author | Youcheng Zhu Di Wang Fan Yan Le Wang Ying Wang Jingwen Li Xuguang Yang Ziwei Gao Xu Liu Yajing Liu Qingyu Wang |
| author_facet | Youcheng Zhu Di Wang Fan Yan Le Wang Ying Wang Jingwen Li Xuguang Yang Ziwei Gao Xu Liu Yajing Liu Qingyu Wang |
| author_sort | Youcheng Zhu |
| collection | DOAJ |
| description | Abstract We aimed to identify HD‐Zip (homologous domain leucine zipper) family genes based on the complete Sophora alopecuroides genome sequence. Eighty‐six Sophora alopecuroides HD‐Zip family (SaHDZ) genes were identified and categorized into four subclasses using phylogenetic analysis. Chromosome localization analysis revealed that these genes were distributed across 18 chromosomes. Gene structure and conserved motif analysis showed high similarity among members of the SaHDZ genes. Prediction analysis revealed 71 cis‐acting elements in SaHDZ genes. Transcriptome and quantitative real‐time polymerase chain reaction analyses showed that under salt stress, SaHDZ responded positively in S. alopecuroides, and that SaHDZ22 was significantly upregulated afterward. Functional verification experiments revealed that SaHDZ22 overexpression increased the tolerance of Arabidopsis to salt and osmotic stress. Combined with cis‐acting element prediction and expression level analysis, HD‐Zip family transcription factors may be involved in regulating the balance between plant growth and stress resistance under salt stress by modulating the expression of auxin and abscisic acid signaling pathway genes. The Sophora alopecuroides adenylate kinase protein (SaAKI) and S. alopecuroides tetrapeptide‐like repeat protein (SaTPR; pCAMBIA1300‐SaTPR‐cLUC) expression levels were consistent with those of SaHDZ22, indicating that SaHDZ22 may coordinate with SaAKI and SaTPR to regulate plant salt tolerance. These results lay a foundation in understanding the salt stress response mechanisms of S. alopecuroides and provide a reference for future studies oriented toward exploring plant stress resistance. |
| format | Article |
| id | doaj-art-411dfe0598d54325ab0b58557d31ba5e |
| institution | Kabale University |
| issn | 1940-3372 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Plant Genome |
| spelling | doaj-art-411dfe0598d54325ab0b58557d31ba5e2024-11-17T09:46:07ZengWileyThe Plant Genome1940-33722024-09-01173n/an/a10.1002/tpg2.20504Genome‐wide analysis of HD‐Zip genes in Sophora alopecuroides and their role in salt stress responseYoucheng Zhu0Di Wang1Fan Yan2Le Wang3Ying Wang4Jingwen Li5Xuguang Yang6Ziwei Gao7Xu Liu8Yajing Liu9Qingyu Wang10College of Biological and Agricultural Engineering Jilin University Changchun ChinaCollege of Plant Science Jilin University Changchun ChinaCollege of Plant Science Jilin University Changchun ChinaCollege of Plant Science Jilin University Changchun ChinaCollege of Plant Science Jilin University Changchun ChinaCollege of Plant Science Jilin University Changchun ChinaCollege of Plant Science Jilin University Changchun ChinaCollege of Plant Science Jilin University Changchun ChinaCollege of Plant Science Jilin University Changchun ChinaCollege of Plant Science Jilin University Changchun ChinaCollege of Plant Science Jilin University Changchun ChinaAbstract We aimed to identify HD‐Zip (homologous domain leucine zipper) family genes based on the complete Sophora alopecuroides genome sequence. Eighty‐six Sophora alopecuroides HD‐Zip family (SaHDZ) genes were identified and categorized into four subclasses using phylogenetic analysis. Chromosome localization analysis revealed that these genes were distributed across 18 chromosomes. Gene structure and conserved motif analysis showed high similarity among members of the SaHDZ genes. Prediction analysis revealed 71 cis‐acting elements in SaHDZ genes. Transcriptome and quantitative real‐time polymerase chain reaction analyses showed that under salt stress, SaHDZ responded positively in S. alopecuroides, and that SaHDZ22 was significantly upregulated afterward. Functional verification experiments revealed that SaHDZ22 overexpression increased the tolerance of Arabidopsis to salt and osmotic stress. Combined with cis‐acting element prediction and expression level analysis, HD‐Zip family transcription factors may be involved in regulating the balance between plant growth and stress resistance under salt stress by modulating the expression of auxin and abscisic acid signaling pathway genes. The Sophora alopecuroides adenylate kinase protein (SaAKI) and S. alopecuroides tetrapeptide‐like repeat protein (SaTPR; pCAMBIA1300‐SaTPR‐cLUC) expression levels were consistent with those of SaHDZ22, indicating that SaHDZ22 may coordinate with SaAKI and SaTPR to regulate plant salt tolerance. These results lay a foundation in understanding the salt stress response mechanisms of S. alopecuroides and provide a reference for future studies oriented toward exploring plant stress resistance.https://doi.org/10.1002/tpg2.20504 |
| spellingShingle | Youcheng Zhu Di Wang Fan Yan Le Wang Ying Wang Jingwen Li Xuguang Yang Ziwei Gao Xu Liu Yajing Liu Qingyu Wang Genome‐wide analysis of HD‐Zip genes in Sophora alopecuroides and their role in salt stress response The Plant Genome |
| title | Genome‐wide analysis of HD‐Zip genes in Sophora alopecuroides and their role in salt stress response |
| title_full | Genome‐wide analysis of HD‐Zip genes in Sophora alopecuroides and their role in salt stress response |
| title_fullStr | Genome‐wide analysis of HD‐Zip genes in Sophora alopecuroides and their role in salt stress response |
| title_full_unstemmed | Genome‐wide analysis of HD‐Zip genes in Sophora alopecuroides and their role in salt stress response |
| title_short | Genome‐wide analysis of HD‐Zip genes in Sophora alopecuroides and their role in salt stress response |
| title_sort | genome wide analysis of hd zip genes in sophora alopecuroides and their role in salt stress response |
| url | https://doi.org/10.1002/tpg2.20504 |
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