Simultaneous Monitoring of Tyrosinase and ATP in Thick Brain Tissues Using a Single Two‐Photon Fluorescent Probe
Abstract Cellular redox homeostasis and energy metabolism in the central nervous system are associated with neurodegenerative diseases. However, their real‐time and concurrent monitoring in thick tissues remains challenging. Herein, a single dual‐emission two‐photon fluorescent probe (named DST) is...
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Wiley
2025-05-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202413220 |
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| author | Hong Huang Huiru Li Yong Zhang Xuhan Xia Ningwen Zhang Haixin Fan Longhua Guo Yongyong Cao Hu Pan Ruijie Deng Yangang Wang Rodrigo Ledesma‐Amaro Jianguo Xu |
| author_facet | Hong Huang Huiru Li Yong Zhang Xuhan Xia Ningwen Zhang Haixin Fan Longhua Guo Yongyong Cao Hu Pan Ruijie Deng Yangang Wang Rodrigo Ledesma‐Amaro Jianguo Xu |
| author_sort | Hong Huang |
| collection | DOAJ |
| description | Abstract Cellular redox homeostasis and energy metabolism in the central nervous system are associated with neurodegenerative diseases. However, their real‐time and concurrent monitoring in thick tissues remains challenging. Herein, a single dual‐emission two‐photon fluorescent probe (named DST) is designed for the simultaneous tracking of tyrosinase (TYR) and adenosine triphosphate (ATP), thereby enabling the real‐time monitoring of both neurocellular redox homeostasis and energy metabolism in brain tissue. The developed DST probe exhibits excellent sensitivity and selectivity toward TYR and ATP, with distinctive responses in the blue and red fluorescence channels being observed without spectra crosstalk. Using this probe, the correlation and regulatory mechanism between TYR and ATP during oxidative stress are uncovered. Additionally, the two‐photon nature of this probe allows alterations in the TYR and ATP levels to be monitored across different brain regions in an Alzheimer's disease (AD) mouse model. Notably, a significant decrease in ATP levels is revealed within the somatosensory cortex (S1BF) and caudate putamen brain regions of an AD mouse, alongside an increase in TYR levels within the S1BF and laterodorsal thalamic nucleus brain regions. These findings indicate the potential of applying the spatially resolved regulation of neurocellular redox homeostasis and energy metabolism to treat neurodegenerative diseases. |
| format | Article |
| id | doaj-art-411bf1f094064eeaaad35791e257dc3e |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-411bf1f094064eeaaad35791e257dc3e2025-08-20T03:47:33ZengWileyAdvanced Science2198-38442025-05-011219n/an/a10.1002/advs.202413220Simultaneous Monitoring of Tyrosinase and ATP in Thick Brain Tissues Using a Single Two‐Photon Fluorescent ProbeHong Huang0Huiru Li1Yong Zhang2Xuhan Xia3Ningwen Zhang4Haixin Fan5Longhua Guo6Yongyong Cao7Hu Pan8Ruijie Deng9Yangang Wang10Rodrigo Ledesma‐Amaro11Jianguo Xu12College of Biological and Chemical Engineering Jiaxing University Jiaxing 314001 ChinaCollege of Biological and Chemical Engineering Jiaxing University Jiaxing 314001 ChinaCollege of Biomass Science and Engineering Sichuan University Chengdu 610065 ChinaCollege of Biomass Science and Engineering Sichuan University Chengdu 610065 ChinaCollege of Biological and Chemical Engineering Jiaxing University Jiaxing 314001 ChinaCollege of Biological and Chemical Engineering Jiaxing University Jiaxing 314001 ChinaCollege of Biological and Chemical Engineering Jiaxing University Jiaxing 314001 ChinaCollege of Biological and Chemical Engineering Jiaxing University Jiaxing 314001 ChinaCollege of Biological and Chemical Engineering Jiaxing University Jiaxing 314001 ChinaCollege of Biomass Science and Engineering Sichuan University Chengdu 610065 ChinaCollege of Biological and Chemical Engineering Jiaxing University Jiaxing 314001 ChinaDepartment of Bioengineering, Imperial College Centre for Synthetic Biology Imperial College London London SW7 2AZ UKCollege of Biological and Chemical Engineering Jiaxing University Jiaxing 314001 ChinaAbstract Cellular redox homeostasis and energy metabolism in the central nervous system are associated with neurodegenerative diseases. However, their real‐time and concurrent monitoring in thick tissues remains challenging. Herein, a single dual‐emission two‐photon fluorescent probe (named DST) is designed for the simultaneous tracking of tyrosinase (TYR) and adenosine triphosphate (ATP), thereby enabling the real‐time monitoring of both neurocellular redox homeostasis and energy metabolism in brain tissue. The developed DST probe exhibits excellent sensitivity and selectivity toward TYR and ATP, with distinctive responses in the blue and red fluorescence channels being observed without spectra crosstalk. Using this probe, the correlation and regulatory mechanism between TYR and ATP during oxidative stress are uncovered. Additionally, the two‐photon nature of this probe allows alterations in the TYR and ATP levels to be monitored across different brain regions in an Alzheimer's disease (AD) mouse model. Notably, a significant decrease in ATP levels is revealed within the somatosensory cortex (S1BF) and caudate putamen brain regions of an AD mouse, alongside an increase in TYR levels within the S1BF and laterodorsal thalamic nucleus brain regions. These findings indicate the potential of applying the spatially resolved regulation of neurocellular redox homeostasis and energy metabolism to treat neurodegenerative diseases.https://doi.org/10.1002/advs.202413220alzheimer's diseaseATPbrain tissuestwo‐photon imagingtyrosinase |
| spellingShingle | Hong Huang Huiru Li Yong Zhang Xuhan Xia Ningwen Zhang Haixin Fan Longhua Guo Yongyong Cao Hu Pan Ruijie Deng Yangang Wang Rodrigo Ledesma‐Amaro Jianguo Xu Simultaneous Monitoring of Tyrosinase and ATP in Thick Brain Tissues Using a Single Two‐Photon Fluorescent Probe Advanced Science alzheimer's disease ATP brain tissues two‐photon imaging tyrosinase |
| title | Simultaneous Monitoring of Tyrosinase and ATP in Thick Brain Tissues Using a Single Two‐Photon Fluorescent Probe |
| title_full | Simultaneous Monitoring of Tyrosinase and ATP in Thick Brain Tissues Using a Single Two‐Photon Fluorescent Probe |
| title_fullStr | Simultaneous Monitoring of Tyrosinase and ATP in Thick Brain Tissues Using a Single Two‐Photon Fluorescent Probe |
| title_full_unstemmed | Simultaneous Monitoring of Tyrosinase and ATP in Thick Brain Tissues Using a Single Two‐Photon Fluorescent Probe |
| title_short | Simultaneous Monitoring of Tyrosinase and ATP in Thick Brain Tissues Using a Single Two‐Photon Fluorescent Probe |
| title_sort | simultaneous monitoring of tyrosinase and atp in thick brain tissues using a single two photon fluorescent probe |
| topic | alzheimer's disease ATP brain tissues two‐photon imaging tyrosinase |
| url | https://doi.org/10.1002/advs.202413220 |
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