Comparison between Conventional Methods and Molecular Diagnosis for Candida albicans and Candida dubliniensis isolated from Cancer Patients Infected with Oral Candidiasis
Background: Two related pathogenic yeast species, Candida albicans and Candida dubliniensis share many phenotypic and microscopic diagnostic characteristics, in addition to the similarity in the characteristics of diagnosis. Objectives: The present study aimed to diagnose both yeast cells and compar...
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| Main Author: | |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2024-10-01
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| Series: | Medical Journal of Babylon |
| Subjects: | |
| Online Access: | https://doi.org/10.4103/MJBL.MJBL_1079_23 |
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| Summary: | Background: Two related pathogenic yeast species, Candida albicans and Candida dubliniensis share many phenotypic and microscopic diagnostic characteristics, in addition to the similarity in the characteristics of diagnosis. Objectives: The present study aimed to diagnose both yeast cells and compare them at the level of the percentage of similarities and differences according to the traditional methods and molecular approaches, which are considered more accurate and sensitive. Materials and Methods: The ability to form germ tubes was conducted for C. albicans and C. dubliniensis. The identification using biochemical tests, VITEK techniques, and molecular techniques was also conducted. Results: The results showed that both pathogenic yeast cells are similar in phenotypic and biochemical diagnosis, whereas the results of molecular diagnosis showed a difference between them, as well as the results of the drug sensitivity test, showed that the two types of yeast cells are sensitive to the antifungal drugs. The current study also showed the diagnosis of new isolates of the two species for the first time, which can be attributed to the occurrence of genetic mutations in them. Conclusion: Both types of pathogenic yeasts that were diagnosed in the current study are the important pathological types prevalent in cancer patients, especially in oral cavity infections, and they are similar to a large extent in terms of phenotypic and diagnostic characteristics. |
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| ISSN: | 1812-156X 2312-6760 |