Capping Actin Protein Overexpression in Human Colorectal Carcinoma and Its Contributed Tumor Migration
Objective. Human colorectal cancer (CRC) is the third most common cancer; patients with metastatic colorectal cancer (mCRC) show poor prognosis than those with CRC cases. There are no reliable molecular biomarkers for the diagnosis of CRC prognosis except with pathological features. Therefore, it is...
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Wiley
2018-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2018/8623937 |
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| author | Tsung-Jung Tsai Yun-Ping Lim Wen-Ying Chao Chien-Chin Chen Yi-Ju Chen Ching-Yen Lin Ying-Ray Lee |
| author_facet | Tsung-Jung Tsai Yun-Ping Lim Wen-Ying Chao Chien-Chin Chen Yi-Ju Chen Ching-Yen Lin Ying-Ray Lee |
| author_sort | Tsung-Jung Tsai |
| collection | DOAJ |
| description | Objective. Human colorectal cancer (CRC) is the third most common cancer; patients with metastatic colorectal cancer (mCRC) show poor prognosis than those with CRC cases. There are no reliable molecular biomarkers for the diagnosis of CRC prognosis except with pathological features. Therefore, it is urgent to develop a biomarker for diagnosis and/or prediction of human CRC. In addition, capping actin protein (CapG) belongs to the gelsolin family and has been reported to contribute on tumor invasion/metastasis in multiple human cancers. Here, we are the first to evaluate the expression of CapG in human CRCs. Study Design. To investigate the expression levels of CapG in human tissue array by immunohistochemistry (IHC) staining. Moreover, the mRNA and protein levels were also confirmed in four CRC cell lines and determined using real-time RT-PCR and Western blotting. Finally, a Matrigel transwell invasion assay was used to evaluate the invasion ability in CapG high or low expression cells. Results. We demonstrated that CapG could be determined in the normal colon tissue and human CRC specimens. However, CapG was significantly overexpressed in the mCRC specimens compared with that in CRC specimens and normal cases. It was also detectable in the four CRC cell lines including mRNA and protein levels. We also found that knockdown of the expression of CapG reduced tumor migration. Conclusions. In this study, we suggested that CapG could be used as a biomarker for metastatic CRC in the clinical specimens. Moreover, our in vitro study demonstrated that CapG might contribute on tumor metastasis in human CRCs. |
| format | Article |
| id | doaj-art-40f65d6717364f5da5b3ffce3103c4d7 |
| institution | OA Journals |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-40f65d6717364f5da5b3ffce3103c4d72025-08-20T02:07:51ZengWileyAnalytical Cellular Pathology2210-71772210-71852018-01-01201810.1155/2018/86239378623937Capping Actin Protein Overexpression in Human Colorectal Carcinoma and Its Contributed Tumor MigrationTsung-Jung Tsai0Yun-Ping Lim1Wen-Ying Chao2Chien-Chin Chen3Yi-Ju Chen4Ching-Yen Lin5Ying-Ray Lee6Department of Gastroenterology, Ditmanson Medical Foundation Chiayi Christian Hospital, Chiayi, TaiwanDepartment of Pharmacy, College of Pharmacy, China Medical University, Taichung 404, TaiwanDepartment of Nursing, Min-Hwei College of Health Care Management, Tainan 736, TaiwanDepartment of Pathology, Ditmanson Medical Foundation Chiayi Christian Hospital, Chiayi, TaiwanDepartment of Pathology, Ditmanson Medical Foundation Chiayi Christian Hospital, Chiayi, TaiwanDepartment of Medical Research, Ditmanson Medical Foundation Chiayi Christian Hospital, Chiayi, TaiwanDepartment of Nursing, Min-Hwei College of Health Care Management, Tainan 736, TaiwanObjective. Human colorectal cancer (CRC) is the third most common cancer; patients with metastatic colorectal cancer (mCRC) show poor prognosis than those with CRC cases. There are no reliable molecular biomarkers for the diagnosis of CRC prognosis except with pathological features. Therefore, it is urgent to develop a biomarker for diagnosis and/or prediction of human CRC. In addition, capping actin protein (CapG) belongs to the gelsolin family and has been reported to contribute on tumor invasion/metastasis in multiple human cancers. Here, we are the first to evaluate the expression of CapG in human CRCs. Study Design. To investigate the expression levels of CapG in human tissue array by immunohistochemistry (IHC) staining. Moreover, the mRNA and protein levels were also confirmed in four CRC cell lines and determined using real-time RT-PCR and Western blotting. Finally, a Matrigel transwell invasion assay was used to evaluate the invasion ability in CapG high or low expression cells. Results. We demonstrated that CapG could be determined in the normal colon tissue and human CRC specimens. However, CapG was significantly overexpressed in the mCRC specimens compared with that in CRC specimens and normal cases. It was also detectable in the four CRC cell lines including mRNA and protein levels. We also found that knockdown of the expression of CapG reduced tumor migration. Conclusions. In this study, we suggested that CapG could be used as a biomarker for metastatic CRC in the clinical specimens. Moreover, our in vitro study demonstrated that CapG might contribute on tumor metastasis in human CRCs.http://dx.doi.org/10.1155/2018/8623937 |
| spellingShingle | Tsung-Jung Tsai Yun-Ping Lim Wen-Ying Chao Chien-Chin Chen Yi-Ju Chen Ching-Yen Lin Ying-Ray Lee Capping Actin Protein Overexpression in Human Colorectal Carcinoma and Its Contributed Tumor Migration Analytical Cellular Pathology |
| title | Capping Actin Protein Overexpression in Human Colorectal Carcinoma and Its Contributed Tumor Migration |
| title_full | Capping Actin Protein Overexpression in Human Colorectal Carcinoma and Its Contributed Tumor Migration |
| title_fullStr | Capping Actin Protein Overexpression in Human Colorectal Carcinoma and Its Contributed Tumor Migration |
| title_full_unstemmed | Capping Actin Protein Overexpression in Human Colorectal Carcinoma and Its Contributed Tumor Migration |
| title_short | Capping Actin Protein Overexpression in Human Colorectal Carcinoma and Its Contributed Tumor Migration |
| title_sort | capping actin protein overexpression in human colorectal carcinoma and its contributed tumor migration |
| url | http://dx.doi.org/10.1155/2018/8623937 |
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