A voltammetric approach for the quantification of thymoquinone in Nigella Sativa products
Abstract Thymoquinone (TQ), a bioactive compound found in Nigella sativa seed oil, has been widely studied for its therapeutic potential. In this study, a new, sensitive, and cost-effective method was developed for TQ determination using square-wave voltammetry (SWV) with an environmentally friendly...
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Nature Portfolio
2025-05-01
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| Online Access: | https://doi.org/10.1038/s41598-025-03347-3 |
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| author | Michał Świderski Kamila Koszelska Dariusz Guziejewski Kamila Borowczyk Grażyna Chwatko Maciej Ślot Paweł J. Kowalczyk Sylwia Smarzewska |
| author_facet | Michał Świderski Kamila Koszelska Dariusz Guziejewski Kamila Borowczyk Grażyna Chwatko Maciej Ślot Paweł J. Kowalczyk Sylwia Smarzewska |
| author_sort | Michał Świderski |
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| description | Abstract Thymoquinone (TQ), a bioactive compound found in Nigella sativa seed oil, has been widely studied for its therapeutic potential. In this study, a new, sensitive, and cost-effective method was developed for TQ determination using square-wave voltammetry (SWV) with an environmentally friendly carbon paste electrode. Due to the morphology of the TQ oxidation signals, three calibration curve construction methods were tested, namely, based on: current height, peak area and cumulative voltammetry concept. The broadest linear range was established when calibration curve was constructed on the basis of TQ peak current height (LOD 8.9 nmol·L− 1, LOQ 29.8 nmol·L− 1). This method was validated through the analysis of real samples, including Nigella sativa seed oil and dietary supplement, with the results showing a strong correlation to those obtained by the HPLC reference method. The developed method represents a significant advancement in the electrochemical detection of TQ, offering practical benefits in terms of simplicity, precision, and cost-effectiveness. |
| format | Article |
| id | doaj-art-40eacda2cbd24e289bd05bcd5e04b430 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-40eacda2cbd24e289bd05bcd5e04b4302025-08-20T03:22:12ZengNature PortfolioScientific Reports2045-23222025-05-0115111110.1038/s41598-025-03347-3A voltammetric approach for the quantification of thymoquinone in Nigella Sativa productsMichał Świderski0Kamila Koszelska1Dariusz Guziejewski2Kamila Borowczyk3Grażyna Chwatko4Maciej Ślot5Paweł J. Kowalczyk6Sylwia Smarzewska7Doctoral School of Exact and Natural Sciences; Faculty of Chemistry, Department of Inorganic and Analytical Chemistry, University of LodzFaculty of Chemistry, Department of Inorganic and Anaytical Chemistry, University of LodzFaculty of Chemistry, Department of Inorganic and Anaytical Chemistry, University of LodzFaculty of Chemistry, Department of Environmental Chemistry, University of LodzFaculty of Chemistry, Department of Environmental Chemistry, University of LodzFaculty of Physics and Applied Informatics, Department of Solid State Physics, University of LodzFaculty of Physics and Applied Informatics, Department of Solid State Physics, University of LodzFaculty of Chemistry, Department of Inorganic and Anaytical Chemistry, University of LodzAbstract Thymoquinone (TQ), a bioactive compound found in Nigella sativa seed oil, has been widely studied for its therapeutic potential. In this study, a new, sensitive, and cost-effective method was developed for TQ determination using square-wave voltammetry (SWV) with an environmentally friendly carbon paste electrode. Due to the morphology of the TQ oxidation signals, three calibration curve construction methods were tested, namely, based on: current height, peak area and cumulative voltammetry concept. The broadest linear range was established when calibration curve was constructed on the basis of TQ peak current height (LOD 8.9 nmol·L− 1, LOQ 29.8 nmol·L− 1). This method was validated through the analysis of real samples, including Nigella sativa seed oil and dietary supplement, with the results showing a strong correlation to those obtained by the HPLC reference method. The developed method represents a significant advancement in the electrochemical detection of TQ, offering practical benefits in terms of simplicity, precision, and cost-effectiveness.https://doi.org/10.1038/s41598-025-03347-3ThymoquinoneVoltammetryCarbon paste electrodeNigella sativaNigella sativa seed oilBlack cumin |
| spellingShingle | Michał Świderski Kamila Koszelska Dariusz Guziejewski Kamila Borowczyk Grażyna Chwatko Maciej Ślot Paweł J. Kowalczyk Sylwia Smarzewska A voltammetric approach for the quantification of thymoquinone in Nigella Sativa products Scientific Reports Thymoquinone Voltammetry Carbon paste electrode Nigella sativa Nigella sativa seed oil Black cumin |
| title | A voltammetric approach for the quantification of thymoquinone in Nigella Sativa products |
| title_full | A voltammetric approach for the quantification of thymoquinone in Nigella Sativa products |
| title_fullStr | A voltammetric approach for the quantification of thymoquinone in Nigella Sativa products |
| title_full_unstemmed | A voltammetric approach for the quantification of thymoquinone in Nigella Sativa products |
| title_short | A voltammetric approach for the quantification of thymoquinone in Nigella Sativa products |
| title_sort | voltammetric approach for the quantification of thymoquinone in nigella sativa products |
| topic | Thymoquinone Voltammetry Carbon paste electrode Nigella sativa Nigella sativa seed oil Black cumin |
| url | https://doi.org/10.1038/s41598-025-03347-3 |
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