IL-10 in Antilipopolysaccharide Immunity Against Systemic Klebsiella Infections

Aim. This study was undertaken in order to determine whether anti-inflammatory cytokine interleukin-10 is responsible for a previously described protection against Klebsiella infection mediated by antilipopolysaccharide antibodies. Methods. BALB/c mice were infected intraperitoneally with a lethal c...

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Main Authors: Tomislav Rukavina, Brigita Ticac, Vanja Vasiljev
Format: Article
Language:English
Published: Wiley 2006-01-01
Series:Mediators of Inflammation
Online Access:http://dx.doi.org/10.1155/MI/2006/69431
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author Tomislav Rukavina
Brigita Ticac
Vanja Vasiljev
author_facet Tomislav Rukavina
Brigita Ticac
Vanja Vasiljev
author_sort Tomislav Rukavina
collection DOAJ
description Aim. This study was undertaken in order to determine whether anti-inflammatory cytokine interleukin-10 is responsible for a previously described protection against Klebsiella infection mediated by antilipopolysaccharide antibodies. Methods. BALB/c mice were infected intraperitoneally with a lethal challenge of Klebsiella pneumoniae Caroli. One group was protected with monoclonal antibodies prior to infection and the second was not. We measured plasma levels of interleukin-10 at different time points by enzyme immunoassay and analyzed the relation between interleukin-10 and proinflammatory cytokines interleukin-6 and tumor necrosis factor-α in order to determine the association of these ratios with the outcome of infection. Major findings and conclusions. We found different pattern of interleukin-10 production in protected mice compared with unprotected ones. The difference is greatest 24 hours postinfection. The ratios between IL-10 and proinflammatory cytokines confirmed the suppressed proinflammatory response in protected animals, especially 24 hours postinfection. Hence the mortality in unprotected mice begins immediately after we conclude that such cytokine relation and IL-10 production are, at least partially, responsible for the destiny of infected animals and the outcome of infection.
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spelling doaj-art-40e42661b7cf4c68b69e8f41f41decf62025-02-03T05:54:11ZengWileyMediators of Inflammation0962-93511466-18612006-01-01200610.1155/MI/2006/6943169431IL-10 in Antilipopolysaccharide Immunity Against Systemic Klebsiella InfectionsTomislav Rukavina0Brigita Ticac1Vanja Vasiljev2Department of Microbiology and Parasitology, Medical Faculty, University of Rijeka, Brace Branchetta 20, Rijeka HR-51 000, CroatiaDepartment of Microbiology and Parasitology, Medical Faculty, University of Rijeka, Brace Branchetta 20, Rijeka HR-51 000, CroatiaDepartment of Microbiology and Parasitology, Medical Faculty, University of Rijeka, Brace Branchetta 20, Rijeka HR-51 000, CroatiaAim. This study was undertaken in order to determine whether anti-inflammatory cytokine interleukin-10 is responsible for a previously described protection against Klebsiella infection mediated by antilipopolysaccharide antibodies. Methods. BALB/c mice were infected intraperitoneally with a lethal challenge of Klebsiella pneumoniae Caroli. One group was protected with monoclonal antibodies prior to infection and the second was not. We measured plasma levels of interleukin-10 at different time points by enzyme immunoassay and analyzed the relation between interleukin-10 and proinflammatory cytokines interleukin-6 and tumor necrosis factor-α in order to determine the association of these ratios with the outcome of infection. Major findings and conclusions. We found different pattern of interleukin-10 production in protected mice compared with unprotected ones. The difference is greatest 24 hours postinfection. The ratios between IL-10 and proinflammatory cytokines confirmed the suppressed proinflammatory response in protected animals, especially 24 hours postinfection. Hence the mortality in unprotected mice begins immediately after we conclude that such cytokine relation and IL-10 production are, at least partially, responsible for the destiny of infected animals and the outcome of infection.http://dx.doi.org/10.1155/MI/2006/69431
spellingShingle Tomislav Rukavina
Brigita Ticac
Vanja Vasiljev
IL-10 in Antilipopolysaccharide Immunity Against Systemic Klebsiella Infections
Mediators of Inflammation
title IL-10 in Antilipopolysaccharide Immunity Against Systemic Klebsiella Infections
title_full IL-10 in Antilipopolysaccharide Immunity Against Systemic Klebsiella Infections
title_fullStr IL-10 in Antilipopolysaccharide Immunity Against Systemic Klebsiella Infections
title_full_unstemmed IL-10 in Antilipopolysaccharide Immunity Against Systemic Klebsiella Infections
title_short IL-10 in Antilipopolysaccharide Immunity Against Systemic Klebsiella Infections
title_sort il 10 in antilipopolysaccharide immunity against systemic klebsiella infections
url http://dx.doi.org/10.1155/MI/2006/69431
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AT brigitaticac il10inantilipopolysaccharideimmunityagainstsystemicklebsiellainfections
AT vanjavasiljev il10inantilipopolysaccharideimmunityagainstsystemicklebsiellainfections