CB-MNCs@ CS/HEC/GP promote wound healing in aged murine pressure ulcer model
Abstract Background Non-healing pressure ulcers impose heavy burdens on patients and clinicians. Cord blood mononuclear cells (CB-MNCs) are a novel type of tissue repair seed cells. However, their clinical application is restricted by low retention and survival rates post-transplantation. This study...
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BMC
2025-02-01
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| Series: | Stem Cell Research & Therapy |
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| Online Access: | https://doi.org/10.1186/s13287-025-04177-w |
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| author | Zhi-cheng Yang He Lin Guo-jun Liu Hui Pan Jun-lu Zhu Xiao-hong Zhang Feng Gao Zhong Wang Zhi-hao Wang |
| author_facet | Zhi-cheng Yang He Lin Guo-jun Liu Hui Pan Jun-lu Zhu Xiao-hong Zhang Feng Gao Zhong Wang Zhi-hao Wang |
| author_sort | Zhi-cheng Yang |
| collection | DOAJ |
| description | Abstract Background Non-healing pressure ulcers impose heavy burdens on patients and clinicians. Cord blood mononuclear cells (CB-MNCs) are a novel type of tissue repair seed cells. However, their clinical application is restricted by low retention and survival rates post-transplantation. This study aims to investigate the role of thermo-sensitive chitosan/hydroxyethyl cellulose/glycerophosphate (CS/HEC/GP) hydrogel encapsulated CB-MNCs in pressure ulcer wound healing. Methods Pressure ulcers were induced on the backs of aged mice. After construction and validation of the characterization of thermo-sensitive CS/HEC/GP hydrogel, CB-MNCs are encapsulated in the hydrogel, called CB-MNCs@CS/HEC/GP which was locally applied to the mouse wounds. Mouse skin tissues were harvested for histological and molecular biology analyses. Results CB-MNCs@CS/HEC/GP therapy accelerated pressure ulcer wound healing, attenuated inflammatory responses, promoted cell proliferation, angiogenesis, and collagen synthesis. Further investigation revealed that CB-MNCs@CS/HEC/GP exerted therapeutic effects by promoting changes in cell types, including fibroblasts, endothelial cells, keratinocytes, and smooth muscle cells. Conclusion CB-MNCs@CS/HEC/GP enhanced the delivery efficiency of CB-MNCs, preserved the cell viability, and contributed to pressure ulcer wound healing. Thus, CB-MNCs@CS/HEC/GP represents a novel therapeutic approach for skin regeneration of chronic wounds. |
| format | Article |
| id | doaj-art-40c6af62f3dd467c865ad35e50200aef |
| institution | Kabale University |
| issn | 1757-6512 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj-art-40c6af62f3dd467c865ad35e50200aef2025-02-09T12:15:37ZengBMCStem Cell Research & Therapy1757-65122025-02-0116112010.1186/s13287-025-04177-wCB-MNCs@ CS/HEC/GP promote wound healing in aged murine pressure ulcer modelZhi-cheng Yang0He Lin1Guo-jun Liu2Hui Pan3Jun-lu Zhu4Xiao-hong Zhang5Feng Gao6Zhong Wang7Zhi-hao Wang8Department of Geriatric Medicine & Laboratory of Gerontology and Anti-Aging Research, Qilu Hospital of Shandong UniversityDepartment of Geriatric Medicine & Laboratory of Gerontology and Anti-Aging Research, Qilu Hospital of Shandong UniversityShandong Qilu Stem Cell Engineering Co., LtdDepartment of Geriatric Medicine & Laboratory of Gerontology and Anti-Aging Research, Qilu Hospital of Shandong UniversitySchool of Nursing and Rehabilitation, Shandong UniversitySchool of Nursing and Rehabilitation, Shandong UniversityShandong Qilu Stem Cell Engineering Co., LtdShandong Qilu Stem Cell Engineering Co., LtdDepartment of Geriatric Medicine & Laboratory of Gerontology and Anti-Aging Research, Qilu Hospital of Shandong UniversityAbstract Background Non-healing pressure ulcers impose heavy burdens on patients and clinicians. Cord blood mononuclear cells (CB-MNCs) are a novel type of tissue repair seed cells. However, their clinical application is restricted by low retention and survival rates post-transplantation. This study aims to investigate the role of thermo-sensitive chitosan/hydroxyethyl cellulose/glycerophosphate (CS/HEC/GP) hydrogel encapsulated CB-MNCs in pressure ulcer wound healing. Methods Pressure ulcers were induced on the backs of aged mice. After construction and validation of the characterization of thermo-sensitive CS/HEC/GP hydrogel, CB-MNCs are encapsulated in the hydrogel, called CB-MNCs@CS/HEC/GP which was locally applied to the mouse wounds. Mouse skin tissues were harvested for histological and molecular biology analyses. Results CB-MNCs@CS/HEC/GP therapy accelerated pressure ulcer wound healing, attenuated inflammatory responses, promoted cell proliferation, angiogenesis, and collagen synthesis. Further investigation revealed that CB-MNCs@CS/HEC/GP exerted therapeutic effects by promoting changes in cell types, including fibroblasts, endothelial cells, keratinocytes, and smooth muscle cells. Conclusion CB-MNCs@CS/HEC/GP enhanced the delivery efficiency of CB-MNCs, preserved the cell viability, and contributed to pressure ulcer wound healing. Thus, CB-MNCs@CS/HEC/GP represents a novel therapeutic approach for skin regeneration of chronic wounds.https://doi.org/10.1186/s13287-025-04177-wWound healingAgedPressure ulcersCord blood mononuclear cellsThermo-sensitive hydrogel |
| spellingShingle | Zhi-cheng Yang He Lin Guo-jun Liu Hui Pan Jun-lu Zhu Xiao-hong Zhang Feng Gao Zhong Wang Zhi-hao Wang CB-MNCs@ CS/HEC/GP promote wound healing in aged murine pressure ulcer model Stem Cell Research & Therapy Wound healing Aged Pressure ulcers Cord blood mononuclear cells Thermo-sensitive hydrogel |
| title | CB-MNCs@ CS/HEC/GP promote wound healing in aged murine pressure ulcer model |
| title_full | CB-MNCs@ CS/HEC/GP promote wound healing in aged murine pressure ulcer model |
| title_fullStr | CB-MNCs@ CS/HEC/GP promote wound healing in aged murine pressure ulcer model |
| title_full_unstemmed | CB-MNCs@ CS/HEC/GP promote wound healing in aged murine pressure ulcer model |
| title_short | CB-MNCs@ CS/HEC/GP promote wound healing in aged murine pressure ulcer model |
| title_sort | cb mncs cs hec gp promote wound healing in aged murine pressure ulcer model |
| topic | Wound healing Aged Pressure ulcers Cord blood mononuclear cells Thermo-sensitive hydrogel |
| url | https://doi.org/10.1186/s13287-025-04177-w |
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