Exploration of Suitable Conditions for Shoot Proliferation and Rooting of <i>Quercus robur</i> L. in Plant Tissue Culture Technology
<i>Quercus robur</i> L., also referred to as “summer oak” or “English oak”, is an esthetically pleasing species, making it an excellent choice for street trees and gardens. Raising <i>Quercus</i> presents several challenges, including its long growth period, delayed germinati...
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2025-02-01
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| author | Ting Wang Hao Li Jiujiu Zhao Jinliang Huang Yu Zhong Zhenfeng Xu Fang He |
| author_facet | Ting Wang Hao Li Jiujiu Zhao Jinliang Huang Yu Zhong Zhenfeng Xu Fang He |
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| description | <i>Quercus robur</i> L., also referred to as “summer oak” or “English oak”, is an esthetically pleasing species, making it an excellent choice for street trees and gardens. Raising <i>Quercus</i> presents several challenges, including its long growth period, delayed germination, and inconsistent emergence. The shoot proliferation and adventitious root formation of <i>Q. robur</i> are crucial for establishing a tissue culture regeneration system and are vital for the successful transplantation of seedlings. To address this, experiments were conducted to assess shoot proliferation and adventitious root formation in <i>Q. robur</i> using various media. The shoot proliferation time, shoot proliferation coefficient, number of rooting strips, and length indicators of roots were recorded. The results indicated that a combination of 0.3 mg/L 6-Benzylaminopurine (6-BA) and 100 mg/L cefotaxime (Cef) was optimal for shoot propagation, while a solution of 0.1 mg/L 1-Naphthaleneacetic acid (NAA) and 1/2 Murashige and Skoog Medium (1/2MS) medium was most effective for root induction. This study has identified the optimal conditions for adventitious root formation and shoot proliferation in <i>Q. robur</i>, providing a basis for further research into propagation, germplasm conservation and genetic transformation techniques. |
| format | Article |
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| language | English |
| publishDate | 2025-02-01 |
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| spelling | doaj-art-40bf71fb2ed7493883b3d917d904c7352025-08-20T02:42:32ZengMDPI AGLife2075-17292025-02-0115334810.3390/life15030348Exploration of Suitable Conditions for Shoot Proliferation and Rooting of <i>Quercus robur</i> L. in Plant Tissue Culture TechnologyTing Wang0Hao Li1Jiujiu Zhao2Jinliang Huang3Yu Zhong4Zhenfeng Xu5Fang He6National Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River & Forestry Ecological Engineering in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, College of Forestry, Sichuan Agricultural University, Chengdu 611130, ChinaNational Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River & Forestry Ecological Engineering in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, College of Forestry, Sichuan Agricultural University, Chengdu 611130, ChinaNational Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River & Forestry Ecological Engineering in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, College of Forestry, Sichuan Agricultural University, Chengdu 611130, ChinaNational Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River & Forestry Ecological Engineering in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, College of Forestry, Sichuan Agricultural University, Chengdu 611130, ChinaNational Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River & Forestry Ecological Engineering in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, College of Forestry, Sichuan Agricultural University, Chengdu 611130, ChinaNational Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River & Forestry Ecological Engineering in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, College of Forestry, Sichuan Agricultural University, Chengdu 611130, ChinaNational Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River & Forestry Ecological Engineering in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, College of Forestry, Sichuan Agricultural University, Chengdu 611130, China<i>Quercus robur</i> L., also referred to as “summer oak” or “English oak”, is an esthetically pleasing species, making it an excellent choice for street trees and gardens. Raising <i>Quercus</i> presents several challenges, including its long growth period, delayed germination, and inconsistent emergence. The shoot proliferation and adventitious root formation of <i>Q. robur</i> are crucial for establishing a tissue culture regeneration system and are vital for the successful transplantation of seedlings. To address this, experiments were conducted to assess shoot proliferation and adventitious root formation in <i>Q. robur</i> using various media. The shoot proliferation time, shoot proliferation coefficient, number of rooting strips, and length indicators of roots were recorded. The results indicated that a combination of 0.3 mg/L 6-Benzylaminopurine (6-BA) and 100 mg/L cefotaxime (Cef) was optimal for shoot propagation, while a solution of 0.1 mg/L 1-Naphthaleneacetic acid (NAA) and 1/2 Murashige and Skoog Medium (1/2MS) medium was most effective for root induction. This study has identified the optimal conditions for adventitious root formation and shoot proliferation in <i>Q. robur</i>, providing a basis for further research into propagation, germplasm conservation and genetic transformation techniques.https://www.mdpi.com/2075-1729/15/3/348<i>Quercus robur</i> L.tissue cultureshoot proliferationrooting |
| spellingShingle | Ting Wang Hao Li Jiujiu Zhao Jinliang Huang Yu Zhong Zhenfeng Xu Fang He Exploration of Suitable Conditions for Shoot Proliferation and Rooting of <i>Quercus robur</i> L. in Plant Tissue Culture Technology Life <i>Quercus robur</i> L. tissue culture shoot proliferation rooting |
| title | Exploration of Suitable Conditions for Shoot Proliferation and Rooting of <i>Quercus robur</i> L. in Plant Tissue Culture Technology |
| title_full | Exploration of Suitable Conditions for Shoot Proliferation and Rooting of <i>Quercus robur</i> L. in Plant Tissue Culture Technology |
| title_fullStr | Exploration of Suitable Conditions for Shoot Proliferation and Rooting of <i>Quercus robur</i> L. in Plant Tissue Culture Technology |
| title_full_unstemmed | Exploration of Suitable Conditions for Shoot Proliferation and Rooting of <i>Quercus robur</i> L. in Plant Tissue Culture Technology |
| title_short | Exploration of Suitable Conditions for Shoot Proliferation and Rooting of <i>Quercus robur</i> L. in Plant Tissue Culture Technology |
| title_sort | exploration of suitable conditions for shoot proliferation and rooting of i quercus robur i l in plant tissue culture technology |
| topic | <i>Quercus robur</i> L. tissue culture shoot proliferation rooting |
| url | https://www.mdpi.com/2075-1729/15/3/348 |
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