Exploration of Suitable Conditions for Shoot Proliferation and Rooting of <i>Quercus robur</i> L. in Plant Tissue Culture Technology

Exploration of Suitable Conditions for Shoot Proliferation and Rooting of <i>Quercus robur</i> L. in Plant Tissue Culture Technology

<i>Quercus robur</i> L., also referred to as “summer oak” or “English oak”, is an esthetically pleasing species, making it an excellent choice for street trees and gardens. Raising <i>Quercus</i> presents several challenges, including its long growth period, delayed germinati...

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Bibliographic Details
Main Authors: Ting Wang, Hao Li, Jiujiu Zhao, Jinliang Huang, Yu Zhong, Zhenfeng Xu, Fang He
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Life
Subjects:
<i>Quercus robur</i> L.
tissue culture
shoot proliferation
rooting
Online Access:https://www.mdpi.com/2075-1729/15/3/348
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Summary:<i>Quercus robur</i> L., also referred to as “summer oak” or “English oak”, is an esthetically pleasing species, making it an excellent choice for street trees and gardens. Raising <i>Quercus</i> presents several challenges, including its long growth period, delayed germination, and inconsistent emergence. The shoot proliferation and adventitious root formation of <i>Q. robur</i> are crucial for establishing a tissue culture regeneration system and are vital for the successful transplantation of seedlings. To address this, experiments were conducted to assess shoot proliferation and adventitious root formation in <i>Q. robur</i> using various media. The shoot proliferation time, shoot proliferation coefficient, number of rooting strips, and length indicators of roots were recorded. The results indicated that a combination of 0.3 mg/L 6-Benzylaminopurine (6-BA) and 100 mg/L cefotaxime (Cef) was optimal for shoot propagation, while a solution of 0.1 mg/L 1-Naphthaleneacetic acid (NAA) and 1/2 Murashige and Skoog Medium (1/2MS) medium was most effective for root induction. This study has identified the optimal conditions for adventitious root formation and shoot proliferation in <i>Q. robur</i>, providing a basis for further research into propagation, germplasm conservation and genetic transformation techniques.
ISSN:2075-1729

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