Neurofilament light chain concentration mediates the association between regional cortical thickness and Parkinson’s disease with excessive daytime sleepiness

BackgroundExcessive daytime sleepiness (EDS) is a common non-motor symptom in Parkinson’s disease (PD) that negatively impacts quality of life. Although biomarkers of brain structure, function, and neurodegeneration have been studied, their interactions in EDS remain unclear. This study explores the...

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Main Authors: Jieyu Chen, Guoliang Jiang, Yongyun Zhu, Chunyu Liang, Chenxi Liu, Jianzhun Chen, Baiyuan Yang, Xinglong Yang
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-08-01
Series:Frontiers in Aging Neuroscience
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Online Access:https://www.frontiersin.org/articles/10.3389/fnagi.2025.1645290/full
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author Jieyu Chen
Guoliang Jiang
Yongyun Zhu
Chunyu Liang
Chenxi Liu
Jianzhun Chen
Baiyuan Yang
Xinglong Yang
author_facet Jieyu Chen
Guoliang Jiang
Yongyun Zhu
Chunyu Liang
Chenxi Liu
Jianzhun Chen
Baiyuan Yang
Xinglong Yang
author_sort Jieyu Chen
collection DOAJ
description BackgroundExcessive daytime sleepiness (EDS) is a common non-motor symptom in Parkinson’s disease (PD) that negatively impacts quality of life. Although biomarkers of brain structure, function, and neurodegeneration have been studied, their interactions in EDS remain unclear. This study explores the relationship between cortical thickness, functional connectivity (FC), and plasma neurofilament light chain (NfL) levels in PD-EDS.Methods36 PD-EDS patients and 100 PD patients without EDS (PD-non-EDS) underwent structural MRI and resting-state FC analysis, with regions of cortical atrophy serving as regions of interest (ROIs). Plasma NfL levels were quantified using high-sensitivity Single Molecule Array (SiMoA™). Mediation analysis was conducted to explore the interplay between NfL levels, neuroimaging markers, and EDS severity, assessed by the Epworth Sleepiness Scale (ESS).ResultsPD-EDS patients exhibited significant cortical thinning in the left supramarginal gyrus (SMG) and right postcentral region (PoCR), along with weakened FC between the left SMG and left PoCR, and between the right PoCR and left inferior frontal gyrus (all p < 0.05). Plasma NfL levels were significantly higher in PD-EDS patients than in those without EDS (p = 0.004) and mediated the relationship between left SMG thickness and EDS severity.ConclusionPlasma NfL levels mediate the association between cortical thinning in the left SMG and EDS severity in PD-EDS, suggesting a link between neurodegenerative processes underlying axonal injury and cortical atrophy in key regions associated with EDS in PD. Our findings suggest that combining neuroimaging markers with plasma NfL levels may provide valuable insights into the mechanisms driving EDS progression in PD.
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spelling doaj-art-40be8758a7a84e88b6f2d797c7b1bd552025-08-20T03:36:41ZengFrontiers Media S.A.Frontiers in Aging Neuroscience1663-43652025-08-011710.3389/fnagi.2025.16452901645290Neurofilament light chain concentration mediates the association between regional cortical thickness and Parkinson’s disease with excessive daytime sleepinessJieyu Chen0Guoliang Jiang1Yongyun Zhu2Chunyu Liang3Chenxi Liu4Jianzhun Chen5Baiyuan Yang6Xinglong Yang7Department of Neurology, The First Affiliated Hospital of Kunming Medical University, Kunming, ChinaDepartment of Neurosurgery, The First Affiliated Hospital of Kunming Medical University, Kunming, ChinaDepartment of Neurology, The First Affiliated Hospital of Kunming Medical University, Kunming, ChinaDepartment of Neurology, The First Affiliated Hospital of Kunming Medical University, Kunming, ChinaDepartment of Neurology, The First Affiliated Hospital of Kunming Medical University, Kunming, ChinaDepartment of Neurology, The First Affiliated Hospital of Kunming Medical University, Kunming, ChinaDepartment of Neurology, Seventh People's Hospital of Chengdu, Sichuan, ChinaDepartment of Neurology, The First Affiliated Hospital of Kunming Medical University, Kunming, ChinaBackgroundExcessive daytime sleepiness (EDS) is a common non-motor symptom in Parkinson’s disease (PD) that negatively impacts quality of life. Although biomarkers of brain structure, function, and neurodegeneration have been studied, their interactions in EDS remain unclear. This study explores the relationship between cortical thickness, functional connectivity (FC), and plasma neurofilament light chain (NfL) levels in PD-EDS.Methods36 PD-EDS patients and 100 PD patients without EDS (PD-non-EDS) underwent structural MRI and resting-state FC analysis, with regions of cortical atrophy serving as regions of interest (ROIs). Plasma NfL levels were quantified using high-sensitivity Single Molecule Array (SiMoA™). Mediation analysis was conducted to explore the interplay between NfL levels, neuroimaging markers, and EDS severity, assessed by the Epworth Sleepiness Scale (ESS).ResultsPD-EDS patients exhibited significant cortical thinning in the left supramarginal gyrus (SMG) and right postcentral region (PoCR), along with weakened FC between the left SMG and left PoCR, and between the right PoCR and left inferior frontal gyrus (all p < 0.05). Plasma NfL levels were significantly higher in PD-EDS patients than in those without EDS (p = 0.004) and mediated the relationship between left SMG thickness and EDS severity.ConclusionPlasma NfL levels mediate the association between cortical thinning in the left SMG and EDS severity in PD-EDS, suggesting a link between neurodegenerative processes underlying axonal injury and cortical atrophy in key regions associated with EDS in PD. Our findings suggest that combining neuroimaging markers with plasma NfL levels may provide valuable insights into the mechanisms driving EDS progression in PD.https://www.frontiersin.org/articles/10.3389/fnagi.2025.1645290/fullcortical thicknessmediation analysisParkinson’s diseaseneurofilament light chainexcessive daytime sleepinessfunctional connectivity
spellingShingle Jieyu Chen
Guoliang Jiang
Yongyun Zhu
Chunyu Liang
Chenxi Liu
Jianzhun Chen
Baiyuan Yang
Xinglong Yang
Neurofilament light chain concentration mediates the association between regional cortical thickness and Parkinson’s disease with excessive daytime sleepiness
Frontiers in Aging Neuroscience
cortical thickness
mediation analysis
Parkinson’s disease
neurofilament light chain
excessive daytime sleepiness
functional connectivity
title Neurofilament light chain concentration mediates the association between regional cortical thickness and Parkinson’s disease with excessive daytime sleepiness
title_full Neurofilament light chain concentration mediates the association between regional cortical thickness and Parkinson’s disease with excessive daytime sleepiness
title_fullStr Neurofilament light chain concentration mediates the association between regional cortical thickness and Parkinson’s disease with excessive daytime sleepiness
title_full_unstemmed Neurofilament light chain concentration mediates the association between regional cortical thickness and Parkinson’s disease with excessive daytime sleepiness
title_short Neurofilament light chain concentration mediates the association between regional cortical thickness and Parkinson’s disease with excessive daytime sleepiness
title_sort neurofilament light chain concentration mediates the association between regional cortical thickness and parkinson s disease with excessive daytime sleepiness
topic cortical thickness
mediation analysis
Parkinson’s disease
neurofilament light chain
excessive daytime sleepiness
functional connectivity
url https://www.frontiersin.org/articles/10.3389/fnagi.2025.1645290/full
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