Genome-Wide Analysis of Soybean <i>GS3</i>-Family Genes and Functional Characterization of <i>GmGS3-1</i> Responses to Saline and Drought Stresses

Genome-Wide Analysis of Soybean <i>GS3</i>-Family Genes and Functional Characterization of <i>GmGS3-1</i> Responses to Saline and Drought Stresses

The GS3 protein is a G protein widely conserved in plants, playing a pivotal role in growth, development, and stress responses. With the protein sequence of the <i>AT1/GS3</i> gene in sorghum as a query, this study identified five <i>GS3</i> gene family members in the soybean...

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Bibliographic Details
Main Authors: Ting Jin, Kai Zhang, Xiaoshuai Hao, Xiujie Zhang, Shichao Wang, Weihua Long
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Agriculture
Subjects:
GS3
soybean
gene expression
salt stress
drought stress
Online Access:https://www.mdpi.com/2077-0472/15/5/443
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Summary:The GS3 protein is a G protein widely conserved in plants, playing a pivotal role in growth, development, and stress responses. With the protein sequence of the <i>AT1/GS3</i> gene in sorghum as a query, this study identified five <i>GS3</i> gene family members in the soybean genome database and designated <i>GmGS3-1</i> to <i>GmGS3-5</i>. Promoter cis-element analysis suggested that soybean <i>GS3</i> is implicated in responses to abiotic stress. Additionally, collinearity analysis indicated that soybean <i>GS3</i> genes are subject to purifying selection. Transcriptomic data of the five soybean <i>GS3</i> genes revealed that the nuclear-localized gene <i>GmGS3-1</i> is highly expressed in root tissues and significantly upregulated under salt and drought stress, as confirmed by qRT-PCR assays. Functional validation for salt and drought tolerance demonstrated that transgenic <i>Arabidopsis</i> plants exhibited higher fresh weight compared to wild-type (WT) controls. Furthermore, GmGS3-1 was found to interact with multiple G proteins, suggesting its synergistic role in the abiotic stress tolerance of soybean. These findings establish a theoretical framework for understanding the functional role of the <i>GS3</i> gene family in soybean stress tolerance and development.
ISSN:2077-0472

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