Revolutionizing Tissue Clearing and 3-Dimensional Imaging: Transparent Embedding Solvent System for Uniform High-Resolution Imaging
Combining transparent embedding with sectioning is likely to be the future direction for tissue clearing and 3-dimensional (3D) imaging. A newly published transparent embedding system, TESOS (Transparent Embedding Solvent System), ensures consistent submicron resolution imaging throughout the entire...
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| Format: | Article |
| Language: | English |
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American Association for the Advancement of Science (AAAS)
2025-01-01
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| Series: | BME Frontiers |
| Online Access: | https://spj.science.org/doi/10.34133/bmef.0095 |
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| _version_ | 1832590739247202304 |
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| author | Yating Yi Hu Zhao |
| author_facet | Yating Yi Hu Zhao |
| author_sort | Yating Yi |
| collection | DOAJ |
| description | Combining transparent embedding with sectioning is likely to be the future direction for tissue clearing and 3-dimensional (3D) imaging. A newly published transparent embedding system, TESOS (Transparent Embedding Solvent System), ensures consistent submicron resolution imaging throughout the entire sample, and can be compatible with different microscopy systems. This method shows great potential in connectome mapping, and might be an optimal option for future 3D multiplex immunofluorescence and RNA in situ hybridization imaging. Additional efforts would be needed to innovate labeling, imaging, and data processing strategies to fully utilize the potential of transparent embedding systems in high-resolution imaging of large-scale samples. |
| format | Article |
| id | doaj-art-40bd639d63994140b0719d1e0002efe8 |
| institution | Kabale University |
| issn | 2765-8031 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | Article |
| series | BME Frontiers |
| spelling | doaj-art-40bd639d63994140b0719d1e0002efe82025-01-23T08:00:30ZengAmerican Association for the Advancement of Science (AAAS)BME Frontiers2765-80312025-01-01610.34133/bmef.0095Revolutionizing Tissue Clearing and 3-Dimensional Imaging: Transparent Embedding Solvent System for Uniform High-Resolution ImagingYating Yi0Hu Zhao1State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China.Chinese Institute for Brain Research, Beijing, China.Combining transparent embedding with sectioning is likely to be the future direction for tissue clearing and 3-dimensional (3D) imaging. A newly published transparent embedding system, TESOS (Transparent Embedding Solvent System), ensures consistent submicron resolution imaging throughout the entire sample, and can be compatible with different microscopy systems. This method shows great potential in connectome mapping, and might be an optimal option for future 3D multiplex immunofluorescence and RNA in situ hybridization imaging. Additional efforts would be needed to innovate labeling, imaging, and data processing strategies to fully utilize the potential of transparent embedding systems in high-resolution imaging of large-scale samples.https://spj.science.org/doi/10.34133/bmef.0095 |
| spellingShingle | Yating Yi Hu Zhao Revolutionizing Tissue Clearing and 3-Dimensional Imaging: Transparent Embedding Solvent System for Uniform High-Resolution Imaging BME Frontiers |
| title | Revolutionizing Tissue Clearing and 3-Dimensional Imaging: Transparent Embedding Solvent System for Uniform High-Resolution Imaging |
| title_full | Revolutionizing Tissue Clearing and 3-Dimensional Imaging: Transparent Embedding Solvent System for Uniform High-Resolution Imaging |
| title_fullStr | Revolutionizing Tissue Clearing and 3-Dimensional Imaging: Transparent Embedding Solvent System for Uniform High-Resolution Imaging |
| title_full_unstemmed | Revolutionizing Tissue Clearing and 3-Dimensional Imaging: Transparent Embedding Solvent System for Uniform High-Resolution Imaging |
| title_short | Revolutionizing Tissue Clearing and 3-Dimensional Imaging: Transparent Embedding Solvent System for Uniform High-Resolution Imaging |
| title_sort | revolutionizing tissue clearing and 3 dimensional imaging transparent embedding solvent system for uniform high resolution imaging |
| url | https://spj.science.org/doi/10.34133/bmef.0095 |
| work_keys_str_mv | AT yatingyi revolutionizingtissueclearingand3dimensionalimagingtransparentembeddingsolventsystemforuniformhighresolutionimaging AT huzhao revolutionizingtissueclearingand3dimensionalimagingtransparentembeddingsolventsystemforuniformhighresolutionimaging |