Evaluation of cd44 and Pd-L1 expression during co-cultivation of tumor and hematopoietic stem cells
Background. Cd44 molecule is overexpressed on tumor‑associated cells, including stem cells, in the tumor microenvironment, which in most cases is a poor prognostic marker for the tumor progression. In addition, the pd‑L1 molecule positively correlates with Cd44, which is associated with resistance t...
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| Language: | Russian |
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ABV-press
2025-03-01
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| Series: | Онкогематология |
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| Online Access: | https://oncohematology.abvpress.ru/ongm/article/view/1011 |
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| author | M. V. Bykova A. A. Aktanova I. P. Skachkov V. V. Denisova E. A. Pashkina |
| author_facet | M. V. Bykova A. A. Aktanova I. P. Skachkov V. V. Denisova E. A. Pashkina |
| author_sort | M. V. Bykova |
| collection | DOAJ |
| description | Background. Cd44 molecule is overexpressed on tumor‑associated cells, including stem cells, in the tumor microenvironment, which in most cases is a poor prognostic marker for the tumor progression. In addition, the pd‑L1 molecule positively correlates with Cd44, which is associated with resistance to antitumor therapy, so these markers are perspective targets for both diagnostics and therapy of oncological diseases. Aim. To determine the expression of checkpoint molecules and Cd44 during co‑cultivation of tumor and hematopoietic stem cells under various conditions. Materials and methods. Cd34+ hematopoietic stem cells (n = 10) and tumor lines 1301, K562 and SK‑mel37 were used for this study. Samples labeled with monoclonal antibodies to Cd44, pd‑L1 and pd‑1 were analyzed by flow cytometry. Results. The expression of molecules was different with co‑culturing of hematopoietic stem cells with several types of tumors, so the number of Cd34+Cd44+ cells was 3 times lower in the group with SK‑mel37 compared to leukemia 1301 and K562 (the median was 7.1; 22.4 and 22.7, respectively). In addition, the expression of the pd‑L1 molecule on SK‑mel37 was significantly higher than on other tumor cells (p < 0.05). Conclusion. It is necessary to study the patterns of change not only in the expression of these molecules, but also in co‑expression depending on the type and conditions of cells interaction with each other. |
| format | Article |
| id | doaj-art-40b67f5ae2fd4be2a5c97e4855d81acd |
| institution | DOAJ |
| issn | 1818-8346 2413-4023 |
| language | Russian |
| publishDate | 2025-03-01 |
| publisher | ABV-press |
| record_format | Article |
| series | Онкогематология |
| spelling | doaj-art-40b67f5ae2fd4be2a5c97e4855d81acd2025-08-20T03:21:28ZrusABV-pressОнкогематология1818-83462413-40232025-03-0120112212710.17650/1818-8346-2025-20-1-122-127813Evaluation of cd44 and Pd-L1 expression during co-cultivation of tumor and hematopoietic stem cellsM. V. Bykova0A. A. Aktanova1I. P. Skachkov2V. V. Denisova3E. A. Pashkina4Research Institute of Fundamental and Clinical ImmunologyResearch Institute of Fundamental and Clinical Immunology; Novosibirsk State Medical UniversityResearch Institute of Fundamental and Clinical Immunology; Novosibirsk State Medical UniversityClinic of Immunopathology, Research Institute of Fundamental and Clinical ImmunologyResearch Institute of Fundamental and Clinical Immunology; Novosibirsk State Medical UniversityBackground. Cd44 molecule is overexpressed on tumor‑associated cells, including stem cells, in the tumor microenvironment, which in most cases is a poor prognostic marker for the tumor progression. In addition, the pd‑L1 molecule positively correlates with Cd44, which is associated with resistance to antitumor therapy, so these markers are perspective targets for both diagnostics and therapy of oncological diseases. Aim. To determine the expression of checkpoint molecules and Cd44 during co‑cultivation of tumor and hematopoietic stem cells under various conditions. Materials and methods. Cd34+ hematopoietic stem cells (n = 10) and tumor lines 1301, K562 and SK‑mel37 were used for this study. Samples labeled with monoclonal antibodies to Cd44, pd‑L1 and pd‑1 were analyzed by flow cytometry. Results. The expression of molecules was different with co‑culturing of hematopoietic stem cells with several types of tumors, so the number of Cd34+Cd44+ cells was 3 times lower in the group with SK‑mel37 compared to leukemia 1301 and K562 (the median was 7.1; 22.4 and 22.7, respectively). In addition, the expression of the pd‑L1 molecule on SK‑mel37 was significantly higher than on other tumor cells (p < 0.05). Conclusion. It is necessary to study the patterns of change not only in the expression of these molecules, but also in co‑expression depending on the type and conditions of cells interaction with each other.https://oncohematology.abvpress.ru/ongm/article/view/1011cd44+immune checkpointcancer stem celltumor microenvironmenthematopoietic stem cell |
| spellingShingle | M. V. Bykova A. A. Aktanova I. P. Skachkov V. V. Denisova E. A. Pashkina Evaluation of cd44 and Pd-L1 expression during co-cultivation of tumor and hematopoietic stem cells Онкогематология cd44+ immune checkpoint cancer stem cell tumor microenvironment hematopoietic stem cell |
| title | Evaluation of cd44 and Pd-L1 expression during co-cultivation of tumor and hematopoietic stem cells |
| title_full | Evaluation of cd44 and Pd-L1 expression during co-cultivation of tumor and hematopoietic stem cells |
| title_fullStr | Evaluation of cd44 and Pd-L1 expression during co-cultivation of tumor and hematopoietic stem cells |
| title_full_unstemmed | Evaluation of cd44 and Pd-L1 expression during co-cultivation of tumor and hematopoietic stem cells |
| title_short | Evaluation of cd44 and Pd-L1 expression during co-cultivation of tumor and hematopoietic stem cells |
| title_sort | evaluation of cd44 and pd l1 expression during co cultivation of tumor and hematopoietic stem cells |
| topic | cd44+ immune checkpoint cancer stem cell tumor microenvironment hematopoietic stem cell |
| url | https://oncohematology.abvpress.ru/ongm/article/view/1011 |
| work_keys_str_mv | AT mvbykova evaluationofcd44andpdl1expressionduringcocultivationoftumorandhematopoieticstemcells AT aaaktanova evaluationofcd44andpdl1expressionduringcocultivationoftumorandhematopoieticstemcells AT ipskachkov evaluationofcd44andpdl1expressionduringcocultivationoftumorandhematopoieticstemcells AT vvdenisova evaluationofcd44andpdl1expressionduringcocultivationoftumorandhematopoieticstemcells AT eapashkina evaluationofcd44andpdl1expressionduringcocultivationoftumorandhematopoieticstemcells |