Preparation of enzymatic hydrolysate from crayfish shells and its application in the processing of crayfish whole balls

Objective: For enhancing the flavor characteristics and nutritional value of crayfish balls, and enriching the diversity of crayfish processed products. Methods: Determined the process of preparing the enzymatic hydrolysate from crayfish shell using compound enzymatic by single-factor and orthogonal...

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Main Authors: YANG Qi-fu, XU Wen-si, WEI Na-qiang, XU Yan, YANG Pin-hong
Format: Article
Language:English
Published: The Editorial Office of Food and Machinery 2023-06-01
Series:Shipin yu jixie
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Online Access:http://www.ifoodmm.com/spyjxen/article/abstract/20230428
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author YANG Qi-fu
XU Wen-si
WEI Na-qiang
XU Yan
YANG Pin-hong
author_facet YANG Qi-fu
XU Wen-si
WEI Na-qiang
XU Yan
YANG Pin-hong
author_sort YANG Qi-fu
collection DOAJ
description Objective: For enhancing the flavor characteristics and nutritional value of crayfish balls, and enriching the diversity of crayfish processed products. Methods: Determined the process of preparing the enzymatic hydrolysate from crayfish shell using compound enzymatic by single-factor and orthogonal array design. The nutritional quality of crayfish whole balls containing enzyme hydrolysate lyophilized powder and crayfish meat was analyzed. Results: The optimum condition for the preparation of crayfish shells enzymatic hydrolysate solution showed that 1 g crayfish shells powder with 0.5 mL of lactic acid was pretreated at 60 ℃ for 40 min. The dosage of compound enzymatic (papain and flavor protease mass ratio was 3∶1) was 5% (mass fraction), the enzymatic hydrolysis temperature was 60 ℃, time was 4 h, and solid/liquid ratio was 1∶100 (g/mL), and the protein extraction efficiency in the enzymatic hydrolysis was 64.69%. The content of heavy metals in enzyme hydrolysate lyophilized powder was in line with the limit of heavy metals in aquatic products, and contains calcium, magnesium, iron, selenium and other macro and trace elements required by the human body. Its composition analysis showed that the enzymatic hydrolysate lyophilized powder had a certain nutritional value, which could increase the fresh flavor of the food and met the safety standards. The nutrition and texture analysis of crayfish balls showed that the crayfish whole balls had high protein content, rich in a variety of amino acids, comprehensive in nutrition, unique flavor, and with good viscoelasticity and chewiness. Conclusion: The crayfish whole balls added with crayfish shell enzyme hydrolysate lyophilized powder and crayfish meat have a certain nutritional value, which enriches the variety of crayfish ready-to-eat products, improves the utilization rate of whole crayfish.
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spelling doaj-art-40b18de290d845cb9fab9eff797ef7bb2025-08-20T02:21:49ZengThe Editorial Office of Food and MachineryShipin yu jixie1003-57882023-06-0139417017710.13652/j.spjx.1003.5788.2022.80363Preparation of enzymatic hydrolysate from crayfish shells and its application in the processing of crayfish whole ballsYANG Qi-fu0XU Wen-si1WEI Na-qiang2XU Yan3YANG Pin-hong4 College of Life and Environmental Sciences, Hunan University of Arts and Science, Changde, Hunan 415000 , China College of Life and Environmental Sciences, Hunan University of Arts and Science, Changde, Hunan 415000 , China College of Life and Environmental Sciences, Hunan University of Arts and Science, Changde, Hunan 415000 , China College of Life and Environmental Sciences, Hunan University of Arts and Science, Changde, Hunan 415000 , China College of Life and Environmental Sciences, Hunan University of Arts and Science, Changde, Hunan 415000 , China Objective: For enhancing the flavor characteristics and nutritional value of crayfish balls, and enriching the diversity of crayfish processed products. Methods: Determined the process of preparing the enzymatic hydrolysate from crayfish shell using compound enzymatic by single-factor and orthogonal array design. The nutritional quality of crayfish whole balls containing enzyme hydrolysate lyophilized powder and crayfish meat was analyzed. Results: The optimum condition for the preparation of crayfish shells enzymatic hydrolysate solution showed that 1 g crayfish shells powder with 0.5 mL of lactic acid was pretreated at 60 ℃ for 40 min. The dosage of compound enzymatic (papain and flavor protease mass ratio was 3∶1) was 5% (mass fraction), the enzymatic hydrolysis temperature was 60 ℃, time was 4 h, and solid/liquid ratio was 1∶100 (g/mL), and the protein extraction efficiency in the enzymatic hydrolysis was 64.69%. The content of heavy metals in enzyme hydrolysate lyophilized powder was in line with the limit of heavy metals in aquatic products, and contains calcium, magnesium, iron, selenium and other macro and trace elements required by the human body. Its composition analysis showed that the enzymatic hydrolysate lyophilized powder had a certain nutritional value, which could increase the fresh flavor of the food and met the safety standards. The nutrition and texture analysis of crayfish balls showed that the crayfish whole balls had high protein content, rich in a variety of amino acids, comprehensive in nutrition, unique flavor, and with good viscoelasticity and chewiness. Conclusion: The crayfish whole balls added with crayfish shell enzyme hydrolysate lyophilized powder and crayfish meat have a certain nutritional value, which enriches the variety of crayfish ready-to-eat products, improves the utilization rate of whole crayfish.http://www.ifoodmm.com/spyjxen/article/abstract/20230428 compound enzymatic crayfish shell enzymatic hydrolysate crayfish nutrient and flavor processing application
spellingShingle YANG Qi-fu
XU Wen-si
WEI Na-qiang
XU Yan
YANG Pin-hong
Preparation of enzymatic hydrolysate from crayfish shells and its application in the processing of crayfish whole balls
Shipin yu jixie
compound enzymatic
crayfish shell enzymatic hydrolysate
crayfish
nutrient and flavor
processing application
title Preparation of enzymatic hydrolysate from crayfish shells and its application in the processing of crayfish whole balls
title_full Preparation of enzymatic hydrolysate from crayfish shells and its application in the processing of crayfish whole balls
title_fullStr Preparation of enzymatic hydrolysate from crayfish shells and its application in the processing of crayfish whole balls
title_full_unstemmed Preparation of enzymatic hydrolysate from crayfish shells and its application in the processing of crayfish whole balls
title_short Preparation of enzymatic hydrolysate from crayfish shells and its application in the processing of crayfish whole balls
title_sort preparation of enzymatic hydrolysate from crayfish shells and its application in the processing of crayfish whole balls
topic compound enzymatic
crayfish shell enzymatic hydrolysate
crayfish
nutrient and flavor
processing application
url http://www.ifoodmm.com/spyjxen/article/abstract/20230428
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