Pandemic one health clones of Escherichia coli and Klebsiella pneumoniae producing CTX-M-14, CTX-M-27, CTX-M-55 and CTX-M-65 ESβLs among companion animals in northern Ecuador
From a One Health perspective, dogs and cats have begun to be recognized as important reservoirs for clinically significant multidrug-resistant bacterial pathogens. In this study, we investigated the occurrence and genomic features of ESβL producing Enterobacterales isolated from dogs, in the provin...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1259764/full |
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| author | Fernando A. Gonzales-Zubiate José Humberto M. Tambor José Humberto M. Tambor Juan Valencia-Bacca María Fernanda Villota-Burbano Adriana Cardenas-Arias Adriana Cardenas-Arias Fernanda Esposito Fernanda Esposito Quézia Moura Bruna Fuga Bruna Fuga Bruna Fuga Elder Sano Jesus G. M. Pariona Mishell Poleth Ortiz Jacome Nilton Lincopan Nilton Lincopan Nilton Lincopan |
| author_facet | Fernando A. Gonzales-Zubiate José Humberto M. Tambor José Humberto M. Tambor Juan Valencia-Bacca María Fernanda Villota-Burbano Adriana Cardenas-Arias Adriana Cardenas-Arias Fernanda Esposito Fernanda Esposito Quézia Moura Bruna Fuga Bruna Fuga Bruna Fuga Elder Sano Jesus G. M. Pariona Mishell Poleth Ortiz Jacome Nilton Lincopan Nilton Lincopan Nilton Lincopan |
| author_sort | Fernando A. Gonzales-Zubiate |
| collection | DOAJ |
| description | From a One Health perspective, dogs and cats have begun to be recognized as important reservoirs for clinically significant multidrug-resistant bacterial pathogens. In this study, we investigated the occurrence and genomic features of ESβL producing Enterobacterales isolated from dogs, in the province of Imbabura, Ecuador. We identified four isolates expressing ESβLs from healthy and diseased animals. In this regard, two Escherichia coli strains producing CTX-M-55-like or CTX-M-65 ESβLs belonged to the international ST10 and ST162, whereas two Klebsiella pneumoniae producing CTX-M-14 or CTX-M-27 belonged to ST35 and ST661. Phylogenomic analysis clustered (95-105 SNP differences) CTX-M-55/ST10 E. coli from companion animal with food and human E. coli strains of ST10 isolated in 2016, in Australia and Cambodia, respectively; whereas CTX-M-27-positive K. pneumoniae ST661 was clustered (201-216 SNP differences) with human strains identified in Italy, in 2013 and 2017, respectively. In summary, we report the presence and genomic data of global human-associated clones of CTX-M-producing E. coli and K. pneumoniae in dogs, in Ecuador. The implementation of a national epidemiological surveillance program is necessary to establish future strategies to control the dissemination of antibiotic-resistant priority pathogens using a One Health approach. |
| format | Article |
| id | doaj-art-40af3c33ec7544fc963d89c52a86b706 |
| institution | Kabale University |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-40af3c33ec7544fc963d89c52a86b7062025-01-07T08:01:06ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-01-011310.3389/fcimb.2023.12597641259764Pandemic one health clones of Escherichia coli and Klebsiella pneumoniae producing CTX-M-14, CTX-M-27, CTX-M-55 and CTX-M-65 ESβLs among companion animals in northern EcuadorFernando A. Gonzales-Zubiate0José Humberto M. Tambor1José Humberto M. Tambor2Juan Valencia-Bacca3María Fernanda Villota-Burbano4Adriana Cardenas-Arias5Adriana Cardenas-Arias6Fernanda Esposito7Fernanda Esposito8Quézia Moura9Bruna Fuga10Bruna Fuga11Bruna Fuga12Elder Sano13Jesus G. M. Pariona14Mishell Poleth Ortiz Jacome15Nilton Lincopan16Nilton Lincopan17Nilton Lincopan18School of Biological Sciences & Engineering, Yachay Tech University, San Miguel de Urcuquí, EcuadorCentro Universitário ENIAC, São Paulo, BrazilINTI International University, Persiaran Perdana BBN, Nilai, Negeri Sembilan, MalaysiaDepartment of Microbiology and Immunology, Wake Forest School of Medicine, Winston Salem, NC, United StatesDogs & Cats Hospital Veterinario, Ibarra, Imbabura, EcuadorOne Health Brazilian Resistance Project (OneBR), São Paulo, BrazilDepartment of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, BrazilOne Health Brazilian Resistance Project (OneBR), São Paulo, BrazilDepartment of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, BrazilFederal Institute of Espírito Santo, Vila Velha, BrazilOne Health Brazilian Resistance Project (OneBR), São Paulo, BrazilDepartment of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, BrazilDepartment of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, BrazilDepartment of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, BrazilDepartment of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, BrazilSchool of Biological Sciences & Engineering, Yachay Tech University, San Miguel de Urcuquí, EcuadorOne Health Brazilian Resistance Project (OneBR), São Paulo, BrazilDepartment of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, BrazilDepartment of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, BrazilFrom a One Health perspective, dogs and cats have begun to be recognized as important reservoirs for clinically significant multidrug-resistant bacterial pathogens. In this study, we investigated the occurrence and genomic features of ESβL producing Enterobacterales isolated from dogs, in the province of Imbabura, Ecuador. We identified four isolates expressing ESβLs from healthy and diseased animals. In this regard, two Escherichia coli strains producing CTX-M-55-like or CTX-M-65 ESβLs belonged to the international ST10 and ST162, whereas two Klebsiella pneumoniae producing CTX-M-14 or CTX-M-27 belonged to ST35 and ST661. Phylogenomic analysis clustered (95-105 SNP differences) CTX-M-55/ST10 E. coli from companion animal with food and human E. coli strains of ST10 isolated in 2016, in Australia and Cambodia, respectively; whereas CTX-M-27-positive K. pneumoniae ST661 was clustered (201-216 SNP differences) with human strains identified in Italy, in 2013 and 2017, respectively. In summary, we report the presence and genomic data of global human-associated clones of CTX-M-producing E. coli and K. pneumoniae in dogs, in Ecuador. The implementation of a national epidemiological surveillance program is necessary to establish future strategies to control the dissemination of antibiotic-resistant priority pathogens using a One Health approach.https://www.frontiersin.org/articles/10.3389/fcimb.2023.1259764/fullESβLgram-negative bacteriaEnterobacteralesantimicrobial resistanceOne Healthveterinary medicine |
| spellingShingle | Fernando A. Gonzales-Zubiate José Humberto M. Tambor José Humberto M. Tambor Juan Valencia-Bacca María Fernanda Villota-Burbano Adriana Cardenas-Arias Adriana Cardenas-Arias Fernanda Esposito Fernanda Esposito Quézia Moura Bruna Fuga Bruna Fuga Bruna Fuga Elder Sano Jesus G. M. Pariona Mishell Poleth Ortiz Jacome Nilton Lincopan Nilton Lincopan Nilton Lincopan Pandemic one health clones of Escherichia coli and Klebsiella pneumoniae producing CTX-M-14, CTX-M-27, CTX-M-55 and CTX-M-65 ESβLs among companion animals in northern Ecuador Frontiers in Cellular and Infection Microbiology ESβL gram-negative bacteria Enterobacterales antimicrobial resistance One Health veterinary medicine |
| title | Pandemic one health clones of Escherichia coli and Klebsiella pneumoniae producing CTX-M-14, CTX-M-27, CTX-M-55 and CTX-M-65 ESβLs among companion animals in northern Ecuador |
| title_full | Pandemic one health clones of Escherichia coli and Klebsiella pneumoniae producing CTX-M-14, CTX-M-27, CTX-M-55 and CTX-M-65 ESβLs among companion animals in northern Ecuador |
| title_fullStr | Pandemic one health clones of Escherichia coli and Klebsiella pneumoniae producing CTX-M-14, CTX-M-27, CTX-M-55 and CTX-M-65 ESβLs among companion animals in northern Ecuador |
| title_full_unstemmed | Pandemic one health clones of Escherichia coli and Klebsiella pneumoniae producing CTX-M-14, CTX-M-27, CTX-M-55 and CTX-M-65 ESβLs among companion animals in northern Ecuador |
| title_short | Pandemic one health clones of Escherichia coli and Klebsiella pneumoniae producing CTX-M-14, CTX-M-27, CTX-M-55 and CTX-M-65 ESβLs among companion animals in northern Ecuador |
| title_sort | pandemic one health clones of escherichia coli and klebsiella pneumoniae producing ctx m 14 ctx m 27 ctx m 55 and ctx m 65 esβls among companion animals in northern ecuador |
| topic | ESβL gram-negative bacteria Enterobacterales antimicrobial resistance One Health veterinary medicine |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1259764/full |
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