Combined transcriptome and metabolome analysis of chicken follicles in Tengchong Snow Chicken follicle selection
Objective The development of pre-hierarchical follicles (PHFs), especially small yellow follicles (SYFs), directly affects the recruitment of dominant follicles and subsequently affects the egg-laying performance of chickens. The development of PHFs, especially SYFs, and their regulatory mechanisms...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2025-07-01
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| Series: | Animal Bioscience |
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| Online Access: | http://www.animbiosci.org/upload/pdf/ab-24-0861.pdf |
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| author | Yanli Du Xiannian Zi Kun Wang Jinshan Ran Hanyi Xiang Li Lei Yong Liu |
| author_facet | Yanli Du Xiannian Zi Kun Wang Jinshan Ran Hanyi Xiang Li Lei Yong Liu |
| author_sort | Yanli Du |
| collection | DOAJ |
| description | Objective The development of pre-hierarchical follicles (PHFs), especially small yellow follicles (SYFs), directly affects the recruitment of dominant follicles and subsequently affects the egg-laying performance of chickens. The development of PHFs, especially SYFs, and their regulatory mechanisms remain unclear. Methods Transcriptomic and metabolomic analyses were conducted on large white follicles (LWFs) and SYFs in chickens. Results Transcriptome sequencing revealed 258 differentially expressed genes (DEGs) between SYFs and LWFs, with 172 genes upregulated and 86 downregulated. The DEGs were mapped to 17 Kyoto encyclopedia of genes and genomes (KEGG) pathways, including glutathione metabolism, ferroptosis, calcium signaling pathway, and neuroactive ligand–receptor interactions, among others. The metabolome analysis revealed 129 significant differential metabolites (DMs), comprising 36 upregulated and 93 downregulated metabolites. The DMs were associated with nine KEGG pathways, including glutathione metabolism, alpha-linolenic acid metabolism, and linoleic acid metabolism, etc. The combined transcriptional and metabolic analysis revealed significantly enriched pathways. Five KEGG pathways associated with follicular development were identified, including glutathione metabolism, ferroptosis, alpha-linolenic acid metabolism, linoleic acid metabolism, and pyrimidine metabolism. Conclusion Glutathione metabolism may directly inhibit Ferroptosis, which can induce apoptosis of granulosa cells, thereby regulating SYFs development in chickens. These findings could serve as a reference for improving the egg-laying performance of Tengchong snow chickens. |
| format | Article |
| id | doaj-art-40ad8ccbad0347858017ec2b662386ae |
| institution | Kabale University |
| issn | 2765-0189 2765-0235 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Animal Bioscience |
| spelling | doaj-art-40ad8ccbad0347858017ec2b662386ae2025-08-20T03:32:57ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352025-07-013871316132710.5713/ab.24.086125464Combined transcriptome and metabolome analysis of chicken follicles in Tengchong Snow Chicken follicle selectionYanli Du0Xiannian Zi1Kun Wang2Jinshan Ran3Hanyi Xiang4Li Lei5Yong Liu6 College of Agronomy and Life Sciences, Kunming University, Kunming, China College of Animal Science and Technology, Yunnan Agricultural University, Kunming, China College of Animal Science and Technology, Yunnan Agricultural University, Kunming, China College of Agronomy and Life Sciences, Kunming University, Kunming, China Yunnan Rural Revitalizing Education Institute, Yunnan Open University, Kunming, China Yunnan Rural Revitalizing Education Institute, Yunnan Open University, Kunming, China College of Animal Science and Technology, Yunnan Agricultural University, Kunming, ChinaObjective The development of pre-hierarchical follicles (PHFs), especially small yellow follicles (SYFs), directly affects the recruitment of dominant follicles and subsequently affects the egg-laying performance of chickens. The development of PHFs, especially SYFs, and their regulatory mechanisms remain unclear. Methods Transcriptomic and metabolomic analyses were conducted on large white follicles (LWFs) and SYFs in chickens. Results Transcriptome sequencing revealed 258 differentially expressed genes (DEGs) between SYFs and LWFs, with 172 genes upregulated and 86 downregulated. The DEGs were mapped to 17 Kyoto encyclopedia of genes and genomes (KEGG) pathways, including glutathione metabolism, ferroptosis, calcium signaling pathway, and neuroactive ligand–receptor interactions, among others. The metabolome analysis revealed 129 significant differential metabolites (DMs), comprising 36 upregulated and 93 downregulated metabolites. The DMs were associated with nine KEGG pathways, including glutathione metabolism, alpha-linolenic acid metabolism, and linoleic acid metabolism, etc. The combined transcriptional and metabolic analysis revealed significantly enriched pathways. Five KEGG pathways associated with follicular development were identified, including glutathione metabolism, ferroptosis, alpha-linolenic acid metabolism, linoleic acid metabolism, and pyrimidine metabolism. Conclusion Glutathione metabolism may directly inhibit Ferroptosis, which can induce apoptosis of granulosa cells, thereby regulating SYFs development in chickens. These findings could serve as a reference for improving the egg-laying performance of Tengchong snow chickens.http://www.animbiosci.org/upload/pdf/ab-24-0861.pdfegg-laying chickensfolliclemetabolome analysisreverse transcription quantitative polymerase chain reactiontranscriptome analysis |
| spellingShingle | Yanli Du Xiannian Zi Kun Wang Jinshan Ran Hanyi Xiang Li Lei Yong Liu Combined transcriptome and metabolome analysis of chicken follicles in Tengchong Snow Chicken follicle selection Animal Bioscience egg-laying chickens follicle metabolome analysis reverse transcription quantitative polymerase chain reaction transcriptome analysis |
| title | Combined transcriptome and metabolome analysis of chicken follicles in Tengchong Snow Chicken follicle selection |
| title_full | Combined transcriptome and metabolome analysis of chicken follicles in Tengchong Snow Chicken follicle selection |
| title_fullStr | Combined transcriptome and metabolome analysis of chicken follicles in Tengchong Snow Chicken follicle selection |
| title_full_unstemmed | Combined transcriptome and metabolome analysis of chicken follicles in Tengchong Snow Chicken follicle selection |
| title_short | Combined transcriptome and metabolome analysis of chicken follicles in Tengchong Snow Chicken follicle selection |
| title_sort | combined transcriptome and metabolome analysis of chicken follicles in tengchong snow chicken follicle selection |
| topic | egg-laying chickens follicle metabolome analysis reverse transcription quantitative polymerase chain reaction transcriptome analysis |
| url | http://www.animbiosci.org/upload/pdf/ab-24-0861.pdf |
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