Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma
Abstract Sensitive diagnostics are needed to improve management and surveillance of opisthorchiasis and opisthorchiasis-associated cholangiocarcinoma (CCA) throughout East Asia. Herein we generate and screen an Opisthorchis viverrini recombinant secreted proteome to identity antibody biomarkers of l...
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Nature Portfolio
2025-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-61043-2 |
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| author | Lakkhana Sadaow Rutchanee Rodpai Michael J. Smout Rie Nakajima Patcharaporn Boonroumkaew Javier Sotillo Bemnet A. Tedla Vor Luvira Amnat Kitkhuandee Krisada Paonariang Wattana Sukeepaisarnjaroen Hiroshi Yamasaki Sutas Suttiprapa Thewarach Laha Banchob Sripa Rafael de Assis Aarti Jain Wannaporn Ittiprasert Victoria H. Mann Yide Wong Philip L. Felgner Wanchai Maleewong Paul J. Brindley Alex Loukas Pewpan M. Intapan |
| author_facet | Lakkhana Sadaow Rutchanee Rodpai Michael J. Smout Rie Nakajima Patcharaporn Boonroumkaew Javier Sotillo Bemnet A. Tedla Vor Luvira Amnat Kitkhuandee Krisada Paonariang Wattana Sukeepaisarnjaroen Hiroshi Yamasaki Sutas Suttiprapa Thewarach Laha Banchob Sripa Rafael de Assis Aarti Jain Wannaporn Ittiprasert Victoria H. Mann Yide Wong Philip L. Felgner Wanchai Maleewong Paul J. Brindley Alex Loukas Pewpan M. Intapan |
| author_sort | Lakkhana Sadaow |
| collection | DOAJ |
| description | Abstract Sensitive diagnostics are needed to improve management and surveillance of opisthorchiasis and opisthorchiasis-associated cholangiocarcinoma (CCA) throughout East Asia. Herein we generate and screen an Opisthorchis viverrini recombinant secreted proteome to identity antibody biomarkers of liver fluke infection and CCA with sera from study participants in endemic populations and evaluate their utility as point-of-care immunochromatographic tests (PoC-ICTs). We incorporate two of the most promising antigens from the proteome array screen, P1 and P9, into PoC-ICTs to further validate their diagnostic performance. The P9-IgG4 PoC-ICT is superior amongst the single recombinant antigen tests for diagnosing fluke infection as well as fluke-induced CCA, and out-performs parasite crude extract-IgG ICTs. Here we identify two biomarkers of O. viverrini infection and infection-associated CCA that could form the basis of novel antibody serodiagnostic tests for human liver fluke infection and associated cancer. |
| format | Article |
| id | doaj-art-4085b6450ad84e42bf19abcea0edb3f6 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-4085b6450ad84e42bf19abcea0edb3f62025-08-20T03:45:35ZengNature PortfolioNature Communications2041-17232025-07-0116111210.1038/s41467-025-61043-2Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinomaLakkhana Sadaow0Rutchanee Rodpai1Michael J. Smout2Rie Nakajima3Patcharaporn Boonroumkaew4Javier Sotillo5Bemnet A. Tedla6Vor Luvira7Amnat Kitkhuandee8Krisada Paonariang9Wattana Sukeepaisarnjaroen10Hiroshi Yamasaki11Sutas Suttiprapa12Thewarach Laha13Banchob Sripa14Rafael de Assis15Aarti Jain16Wannaporn Ittiprasert17Victoria H. Mann18Yide Wong19Philip L. Felgner20Wanchai Maleewong21Paul J. Brindley22Alex Loukas23Pewpan M. Intapan24Department of Parasitology, Faculty of Medicine, and Mekong Health Science Research Institute, Khon Kaen UniversityDepartment of Parasitology, Faculty of Medicine, and Mekong Health Science Research Institute, Khon Kaen UniversityAustralian Institute of Tropical Health and Medicine, James Cook UniversityVaccine R&D Center, Department of Physiology and Biophysics, University of California IrvineDepartment of Parasitology, Faculty of Medicine, and Mekong Health Science Research Institute, Khon Kaen UniversityParasitology Reference and Research Laboratory, Centro Nacional de Microbiologia, Instituto de Salud Carlos IIIAustralian Institute of Tropical Health and Medicine, James Cook UniversityDepartment of Surgery, Faculty of Medicine, Khon Kaen UniversityDepartment of Surgery, Faculty of Medicine, Khon Kaen UniversityDepartment of Surgery, Faculty of Medicine, Khon Kaen UniversityDepartment of Medicine, Faculty of Medicine, Khon Kaen UniversityDepartment of Parasitology, National Institute of Infectious DiseasesTropical Disease Research Center, Department of Tropical Medicine, Faculty of Medicine, Khon Kaen UniversityDepartment of Parasitology, Faculty of Medicine, and Mekong Health Science Research Institute, Khon Kaen UniversityTropical Disease Research Center, Department of Tropical Medicine, Faculty of Medicine, Khon Kaen UniversityVaccine R&D Center, Department of Physiology and Biophysics, University of California IrvineVaccine R&D Center, Department of Physiology and Biophysics, University of California IrvineDepartment of Microbiology, Immunology and Tropical Medicine, School of Medicine and Health Sciences, George Washington UniversityDepartment of Microbiology, Immunology and Tropical Medicine, School of Medicine and Health Sciences, George Washington UniversityAustralian Institute of Tropical Health and Medicine, James Cook UniversityVaccine R&D Center, Department of Physiology and Biophysics, University of California IrvineDepartment of Parasitology, Faculty of Medicine, and Mekong Health Science Research Institute, Khon Kaen UniversityDepartment of Microbiology, Immunology and Tropical Medicine, School of Medicine and Health Sciences, George Washington UniversityAustralian Institute of Tropical Health and Medicine, James Cook UniversityDepartment of Parasitology, Faculty of Medicine, and Mekong Health Science Research Institute, Khon Kaen UniversityAbstract Sensitive diagnostics are needed to improve management and surveillance of opisthorchiasis and opisthorchiasis-associated cholangiocarcinoma (CCA) throughout East Asia. Herein we generate and screen an Opisthorchis viverrini recombinant secreted proteome to identity antibody biomarkers of liver fluke infection and CCA with sera from study participants in endemic populations and evaluate their utility as point-of-care immunochromatographic tests (PoC-ICTs). We incorporate two of the most promising antigens from the proteome array screen, P1 and P9, into PoC-ICTs to further validate their diagnostic performance. The P9-IgG4 PoC-ICT is superior amongst the single recombinant antigen tests for diagnosing fluke infection as well as fluke-induced CCA, and out-performs parasite crude extract-IgG ICTs. Here we identify two biomarkers of O. viverrini infection and infection-associated CCA that could form the basis of novel antibody serodiagnostic tests for human liver fluke infection and associated cancer.https://doi.org/10.1038/s41467-025-61043-2 |
| spellingShingle | Lakkhana Sadaow Rutchanee Rodpai Michael J. Smout Rie Nakajima Patcharaporn Boonroumkaew Javier Sotillo Bemnet A. Tedla Vor Luvira Amnat Kitkhuandee Krisada Paonariang Wattana Sukeepaisarnjaroen Hiroshi Yamasaki Sutas Suttiprapa Thewarach Laha Banchob Sripa Rafael de Assis Aarti Jain Wannaporn Ittiprasert Victoria H. Mann Yide Wong Philip L. Felgner Wanchai Maleewong Paul J. Brindley Alex Loukas Pewpan M. Intapan Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma Nature Communications |
| title | Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma |
| title_full | Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma |
| title_fullStr | Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma |
| title_full_unstemmed | Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma |
| title_short | Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma |
| title_sort | immunomics guided biomarker discovery for human liver fluke infection and infection associated cholangiocarcinoma |
| url | https://doi.org/10.1038/s41467-025-61043-2 |
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