Features of B cell subsets in primary Sjogren’s syndrome and systemic lupus erythematosus
Background. Lymphocyte subsets in autoimmune diseases, such as systemic lupus erythematosus (SLE) and primary Sjogren’s syndrome (pSS), have been intensively studied in recent years. However, their clinical and diagnostic significance has not been finally determined.The aim of the study – to investi...
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| author | S. S. Benevolenskaya I. V. Kudriavtsev M. K. Serebriakova I. N. Grigor’yeva E. S. Kuvardin A. I. Budkova S. V. Koniakhin D. B. Zammoeva D. B. Motorin A. Yu. Zaritskey I. Z. Gaydukova E. K. Gaydukova S. V. Lapin A. L. Maslyanskiy |
| author_facet | S. S. Benevolenskaya I. V. Kudriavtsev M. K. Serebriakova I. N. Grigor’yeva E. S. Kuvardin A. I. Budkova S. V. Koniakhin D. B. Zammoeva D. B. Motorin A. Yu. Zaritskey I. Z. Gaydukova E. K. Gaydukova S. V. Lapin A. L. Maslyanskiy |
| author_sort | S. S. Benevolenskaya |
| collection | DOAJ |
| description | Background. Lymphocyte subsets in autoimmune diseases, such as systemic lupus erythematosus (SLE) and primary Sjogren’s syndrome (pSS), have been intensively studied in recent years. However, their clinical and diagnostic significance has not been finally determined.The aim of the study – to investigate features and diagnostic utility of B cell subcets in systemic lupus erythematosus and primary Sjogren’s syndrome.Material and methods. A total of 27 SLE patients, 41 pSS patients and 49 healthy volunteers were included in the study. Phenotyping of blood B cell subpopulations was carried out by means of flow cytometry. All peripheral blood B cells were identified by using CD19 antibody, detection of subpopulations of B cells based on expression of IgD, CD38, CD27. Statistical analysis was carried out using Statistica v. 12.0 (StatSoft Inc., USA). The absolute and relative values of B lymphocyte subpopulations were evaluated using three main classifications: based on IgD and CD38 co-expression, IgD and CD27 co-expression as well as CD38 and CD27 co-expression. For comparison of quantitative traits, the Mann – Whitney U-test and Kruskal – Wallis test were used. Method of discriminant analysis was performed to evaluate diagnostic utility of B cell subsets.Results. The most significant discriminant model was obtained using the relative values of all subpopulations.In this model the top significance was documented while assessing the percentage of «unswitched» memory В-cells (IgDdimCD27dim), «naive» (IgDdimCD38low) and activated «naive» B cells (IgDdimCD38dim), germinal center progenitor B cells (IgDhiCD38hi), germinal center B cells (IgDdimCD38hi) and «transient» B cells (CD27lowCD38hi), model percent correct was 75.2% (p<0.05). During ROC analysis, performed for the differential diagnosis of healthy and sick patients, this discriminant model had a sensitivity of 70.6% and a specificity of 85.7%, the area under the curve (AUC) – 0.91 (p<0.001). Among the group of ill patients, distinguishing between SLE and pSS showed a sensitivity of 81.5% and а specificity of 80.5%, AUC=0.84 (p<0.001).Conclusion. B cell subsets might provide an additional diagnostic tool to differentiate between SLE and pSS. |
| format | Article |
| id | doaj-art-407f1bf56a2a437db080c2a4e4506c68 |
| institution | DOAJ |
| issn | 1995-4484 1995-4492 |
| language | Russian |
| publishDate | 2024-11-01 |
| publisher | IMA PRESS LLC |
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| series | Научно-практическая ревматология |
| spelling | doaj-art-407f1bf56a2a437db080c2a4e4506c682025-08-20T02:55:20ZrusIMA PRESS LLCНаучно-практическая ревматология1995-44841995-44922024-11-0162550151210.47360/1995-4484-2024-501-5123019Features of B cell subsets in primary Sjogren’s syndrome and systemic lupus erythematosusS. S. Benevolenskaya0I. V. Kudriavtsev1M. K. Serebriakova2I. N. Grigor’yeva3E. S. Kuvardin4A. I. Budkova5S. V. Koniakhin6D. B. Zammoeva7D. B. Motorin8A. Yu. Zaritskey9I. Z. Gaydukova10E. K. Gaydukova11S. V. Lapin12A. L. Maslyanskiy13Almazov National Medical Research CentreInstitute of Experimental MedicineInstitute of Experimental MedicineAlmazov National Medical Research CentreAlmazov National Medical Research CentrePavlov First Saint Petersburg State Medical UniversityCenter for Theoretical Physics of Complex Systems, Institute for Basic ScienceAlmazov National Medical Research CentreAlmazov National Medical Research CentreAlmazov National Medical Research CentreNorth-Western State Medical University named after I.I. MechnikovSorbonne UniversityPavlov First Saint Petersburg State Medical UniversityAlmazov National Medical Research Centre; Saint Petersburg State UniversityBackground. Lymphocyte subsets in autoimmune diseases, such as systemic lupus erythematosus (SLE) and primary Sjogren’s syndrome (pSS), have been intensively studied in recent years. However, their clinical and diagnostic significance has not been finally determined.The aim of the study – to investigate features and diagnostic utility of B cell subcets in systemic lupus erythematosus and primary Sjogren’s syndrome.Material and methods. A total of 27 SLE patients, 41 pSS patients and 49 healthy volunteers were included in the study. Phenotyping of blood B cell subpopulations was carried out by means of flow cytometry. All peripheral blood B cells were identified by using CD19 antibody, detection of subpopulations of B cells based on expression of IgD, CD38, CD27. Statistical analysis was carried out using Statistica v. 12.0 (StatSoft Inc., USA). The absolute and relative values of B lymphocyte subpopulations were evaluated using three main classifications: based on IgD and CD38 co-expression, IgD and CD27 co-expression as well as CD38 and CD27 co-expression. For comparison of quantitative traits, the Mann – Whitney U-test and Kruskal – Wallis test were used. Method of discriminant analysis was performed to evaluate diagnostic utility of B cell subsets.Results. The most significant discriminant model was obtained using the relative values of all subpopulations.In this model the top significance was documented while assessing the percentage of «unswitched» memory В-cells (IgDdimCD27dim), «naive» (IgDdimCD38low) and activated «naive» B cells (IgDdimCD38dim), germinal center progenitor B cells (IgDhiCD38hi), germinal center B cells (IgDdimCD38hi) and «transient» B cells (CD27lowCD38hi), model percent correct was 75.2% (p<0.05). During ROC analysis, performed for the differential diagnosis of healthy and sick patients, this discriminant model had a sensitivity of 70.6% and a specificity of 85.7%, the area under the curve (AUC) – 0.91 (p<0.001). Among the group of ill patients, distinguishing between SLE and pSS showed a sensitivity of 81.5% and а specificity of 80.5%, AUC=0.84 (p<0.001).Conclusion. B cell subsets might provide an additional diagnostic tool to differentiate between SLE and pSS.https://rsp.mediar-press.net/rsp/article/view/3636lymphocyte subsetsb cellssystemic lupus erythematosusprimary sjogren’s syndromeflow cytometry |
| spellingShingle | S. S. Benevolenskaya I. V. Kudriavtsev M. K. Serebriakova I. N. Grigor’yeva E. S. Kuvardin A. I. Budkova S. V. Koniakhin D. B. Zammoeva D. B. Motorin A. Yu. Zaritskey I. Z. Gaydukova E. K. Gaydukova S. V. Lapin A. L. Maslyanskiy Features of B cell subsets in primary Sjogren’s syndrome and systemic lupus erythematosus Научно-практическая ревматология lymphocyte subsets b cells systemic lupus erythematosus primary sjogren’s syndrome flow cytometry |
| title | Features of B cell subsets in primary Sjogren’s syndrome and systemic lupus erythematosus |
| title_full | Features of B cell subsets in primary Sjogren’s syndrome and systemic lupus erythematosus |
| title_fullStr | Features of B cell subsets in primary Sjogren’s syndrome and systemic lupus erythematosus |
| title_full_unstemmed | Features of B cell subsets in primary Sjogren’s syndrome and systemic lupus erythematosus |
| title_short | Features of B cell subsets in primary Sjogren’s syndrome and systemic lupus erythematosus |
| title_sort | features of b cell subsets in primary sjogren s syndrome and systemic lupus erythematosus |
| topic | lymphocyte subsets b cells systemic lupus erythematosus primary sjogren’s syndrome flow cytometry |
| url | https://rsp.mediar-press.net/rsp/article/view/3636 |
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