Bulk Segregant Analysis Technique to Target Seasonality and Runnering Loci in Diploid Strawberries
Bulked segregant analysis was used to determine inter-simple sequence repeat DNA (ISSR) markers for two genes, one controls seasonality and other runnering in diploid Fragaria spp. ISSR- PCR analysis was performed using these DNA pools as template. The bulks are screened for differences using anchor...
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| Language: | English |
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Tokat Gaziosmanpasa University
2015-09-01
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| Series: | Journal of New Results in Science |
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| Online Access: | https://dergipark.org.tr/en/download/article-file/105215 |
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| _version_ | 1850096012532645888 |
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| author | Cetin Cekic |
| author_facet | Cetin Cekic |
| author_sort | Cetin Cekic |
| collection | DOAJ |
| description | Bulked segregant analysis was used to determine inter-simple sequence repeat DNA (ISSR) markers for two genes, one controls seasonality and other runnering in diploid Fragaria spp. ISSR- PCR analysis was performed using these DNA pools as template. The bulks are screened for differences using anchored simple sequence polymorphic DNA primers which generates clean band profiles and polymorphic fragments were detected. Using 23 primers, either singly or pairwise, we established 16 markers tightly linked to the seasonality locus and 7 markers for runnering locus. This bulked segregant method can be applied for any species and any region of interest in which detailed linkage maps or physical maps are needed. |
| format | Article |
| id | doaj-art-407af42e7c9f482dbfbdf3d2e61f7f26 |
| institution | DOAJ |
| issn | 1304-7981 |
| language | English |
| publishDate | 2015-09-01 |
| publisher | Tokat Gaziosmanpasa University |
| record_format | Article |
| series | Journal of New Results in Science |
| spelling | doaj-art-407af42e7c9f482dbfbdf3d2e61f7f262025-08-20T02:41:19ZengTokat Gaziosmanpasa UniversityJournal of New Results in Science1304-79812015-09-01499298142Bulk Segregant Analysis Technique to Target Seasonality and Runnering Loci in Diploid StrawberriesCetin CekicBulked segregant analysis was used to determine inter-simple sequence repeat DNA (ISSR) markers for two genes, one controls seasonality and other runnering in diploid Fragaria spp. ISSR- PCR analysis was performed using these DNA pools as template. The bulks are screened for differences using anchored simple sequence polymorphic DNA primers which generates clean band profiles and polymorphic fragments were detected. Using 23 primers, either singly or pairwise, we established 16 markers tightly linked to the seasonality locus and 7 markers for runnering locus. This bulked segregant method can be applied for any species and any region of interest in which detailed linkage maps or physical maps are needed.https://dergipark.org.tr/en/download/article-file/105215strawberryissrbulkmarker. |
| spellingShingle | Cetin Cekic Bulk Segregant Analysis Technique to Target Seasonality and Runnering Loci in Diploid Strawberries Journal of New Results in Science strawberry issr bulk marker. |
| title | Bulk Segregant Analysis Technique to Target Seasonality and Runnering Loci in Diploid Strawberries |
| title_full | Bulk Segregant Analysis Technique to Target Seasonality and Runnering Loci in Diploid Strawberries |
| title_fullStr | Bulk Segregant Analysis Technique to Target Seasonality and Runnering Loci in Diploid Strawberries |
| title_full_unstemmed | Bulk Segregant Analysis Technique to Target Seasonality and Runnering Loci in Diploid Strawberries |
| title_short | Bulk Segregant Analysis Technique to Target Seasonality and Runnering Loci in Diploid Strawberries |
| title_sort | bulk segregant analysis technique to target seasonality and runnering loci in diploid strawberries |
| topic | strawberry issr bulk marker. |
| url | https://dergipark.org.tr/en/download/article-file/105215 |
| work_keys_str_mv | AT cetincekic bulksegregantanalysistechniquetotargetseasonalityandrunneringlociindiploidstrawberries |