Snapshot Imaging of Stokes Vector Polarization Speckle in Turbid Optical Phantoms and <i>In Vivo</i> Tissues
Significance: We present a system to measure and analyze the complete polarization state distribution of speckle patterns generated from <i>in vivo</i> tissue. Accurate measurement of polarization speckle requires both precise spatial registration and rapid polarization state acquisition...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
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| Series: | Photonics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-6732/12/1/59 |
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| Summary: | Significance: We present a system to measure and analyze the complete polarization state distribution of speckle patterns generated from <i>in vivo</i> tissue. Accurate measurement of polarization speckle requires both precise spatial registration and rapid polarization state acquisition. A unique measurement system must be designed to achieve accurate images of polarization speckle patterns for detailed investigation of the scattering properties of biological tissues <i>in vivo</i>. Aim and approach: This system features a polarization state analyzer with no moving parts. Two pixel-polarizer cameras allow for the instantaneous acquisition of the spatial Stokes vector distribution of polarization speckle patterns. System design and calibration methods are presented, and representative images from measurements on liquid phantoms (microsphere suspensions) and <i>in vivo</i> healthy and tumor murine models are demonstrated and discussed. Results and Conclusions: Quantitative measurements of polarization speckle from microsphere suspensions with controlled scattering coefficients demonstrate differences in speckle contrast, speckle size, and the degree of polarization. Measurements on <i>in vivo</i> murine skin and xenograft tumor tissue demonstrate the ability of the system to acquire snapshot polarization speckle images in living systems. The developed system can thus rapidly and accurately acquire polarization speckle images from different media in dynamic conditions such as <i>in vivo</i> tissue. This capability opens the potential for future detailed investigation of polarization speckle for <i>in vivo</i> biomedical applications. |
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| ISSN: | 2304-6732 |