Dietary L-proline supplementation ameliorates autism-like behaviors and modulates gut microbiota in the valproic acid-induced mouse model of autism spectrum disorder
The effective intervention strategy for autism spectrum disorder (ASD) are currently limited. Herein, we attempted to evaluate the potential of L-proline (Pro), a multifunctional amino acid, in ameliorating autism-like behaviors and clarify the molecular mechanisms involved by using the typical valp...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tsinghua University Press
2024-09-01
|
| Series: | Food Science and Human Wellness |
| Subjects: | |
| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250234 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841550177145454592 |
|---|---|
| author | Jingjing Fang Ruixuan Geng Seong-Gook Kang Kunlun Huang Tao Tong |
| author_facet | Jingjing Fang Ruixuan Geng Seong-Gook Kang Kunlun Huang Tao Tong |
| author_sort | Jingjing Fang |
| collection | DOAJ |
| description | The effective intervention strategy for autism spectrum disorder (ASD) are currently limited. Herein, we attempted to evaluate the potential of L-proline (Pro), a multifunctional amino acid, in ameliorating autism-like behaviors and clarify the molecular mechanisms involved by using the typical valproic acid (VPA)-induced mouse model of ASD. Pro significantly attenuates repetitive behaviors and social dysfunction in ASD mice. The correlation analysis revealed that the beneficial effects of Pro on autism-like behaviors are related to the modulation of gut microbiota structure and composition. The histological analysis revealed that Pro could reverse the decrease of Nissl-positive cells in the prefrontal cortex (PFC) induced by VPA exposure. RNA sequencing demonstrated that Pro can also alter the PFC transcriptomic profile distinguished by the regulation of genes involved in Parkinson disease, neuroactive ligand-receptor interaction, oxidative phosphorylation, and mitogen activated protein kinase signaling pathway. Overall, dietary Pro supplementation may be a promising intervention strategy for ASD. |
| format | Article |
| id | doaj-art-4060f29a52c745c39553268ef8b91a86 |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-4060f29a52c745c39553268ef8b91a862025-01-10T06:56:56ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-09-011352889290510.26599/FSHW.2022.9250234Dietary L-proline supplementation ameliorates autism-like behaviors and modulates gut microbiota in the valproic acid-induced mouse model of autism spectrum disorderJingjing Fang0Ruixuan Geng1Seong-Gook Kang2Kunlun Huang3Tao Tong4Key Laboratory of Precision Nutrition and Food Quality, Key Laboratory of Functional Dairy, Ministry of Education; College of Food Science and Nutritional Engineering; China Agricultural University, Beijing 100083, ChinaKey Laboratory of Precision Nutrition and Food Quality, Key Laboratory of Functional Dairy, Ministry of Education; College of Food Science and Nutritional Engineering; China Agricultural University, Beijing 100083, ChinaDepartment of Food Engineering and Solar Salt Research Center, Mokpo National University, Muangun 58554, KoreaKey Laboratory of Precision Nutrition and Food Quality, Key Laboratory of Functional Dairy, Ministry of Education; College of Food Science and Nutritional Engineering; China Agricultural University, Beijing 100083, ChinaKey Laboratory of Precision Nutrition and Food Quality, Key Laboratory of Functional Dairy, Ministry of Education; College of Food Science and Nutritional Engineering; China Agricultural University, Beijing 100083, ChinaThe effective intervention strategy for autism spectrum disorder (ASD) are currently limited. Herein, we attempted to evaluate the potential of L-proline (Pro), a multifunctional amino acid, in ameliorating autism-like behaviors and clarify the molecular mechanisms involved by using the typical valproic acid (VPA)-induced mouse model of ASD. Pro significantly attenuates repetitive behaviors and social dysfunction in ASD mice. The correlation analysis revealed that the beneficial effects of Pro on autism-like behaviors are related to the modulation of gut microbiota structure and composition. The histological analysis revealed that Pro could reverse the decrease of Nissl-positive cells in the prefrontal cortex (PFC) induced by VPA exposure. RNA sequencing demonstrated that Pro can also alter the PFC transcriptomic profile distinguished by the regulation of genes involved in Parkinson disease, neuroactive ligand-receptor interaction, oxidative phosphorylation, and mitogen activated protein kinase signaling pathway. Overall, dietary Pro supplementation may be a promising intervention strategy for ASD.https://www.sciopen.com/article/10.26599/FSHW.2022.9250234l-prolineautism spectrum disordergut microbiotaprefrontal cortexbehavior |
| spellingShingle | Jingjing Fang Ruixuan Geng Seong-Gook Kang Kunlun Huang Tao Tong Dietary L-proline supplementation ameliorates autism-like behaviors and modulates gut microbiota in the valproic acid-induced mouse model of autism spectrum disorder Food Science and Human Wellness l-proline autism spectrum disorder gut microbiota prefrontal cortex behavior |
| title | Dietary L-proline supplementation ameliorates autism-like behaviors and modulates gut microbiota in the valproic acid-induced mouse model of autism spectrum disorder |
| title_full | Dietary L-proline supplementation ameliorates autism-like behaviors and modulates gut microbiota in the valproic acid-induced mouse model of autism spectrum disorder |
| title_fullStr | Dietary L-proline supplementation ameliorates autism-like behaviors and modulates gut microbiota in the valproic acid-induced mouse model of autism spectrum disorder |
| title_full_unstemmed | Dietary L-proline supplementation ameliorates autism-like behaviors and modulates gut microbiota in the valproic acid-induced mouse model of autism spectrum disorder |
| title_short | Dietary L-proline supplementation ameliorates autism-like behaviors and modulates gut microbiota in the valproic acid-induced mouse model of autism spectrum disorder |
| title_sort | dietary l proline supplementation ameliorates autism like behaviors and modulates gut microbiota in the valproic acid induced mouse model of autism spectrum disorder |
| topic | l-proline autism spectrum disorder gut microbiota prefrontal cortex behavior |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250234 |
| work_keys_str_mv | AT jingjingfang dietarylprolinesupplementationamelioratesautismlikebehaviorsandmodulatesgutmicrobiotainthevalproicacidinducedmousemodelofautismspectrumdisorder AT ruixuangeng dietarylprolinesupplementationamelioratesautismlikebehaviorsandmodulatesgutmicrobiotainthevalproicacidinducedmousemodelofautismspectrumdisorder AT seonggookkang dietarylprolinesupplementationamelioratesautismlikebehaviorsandmodulatesgutmicrobiotainthevalproicacidinducedmousemodelofautismspectrumdisorder AT kunlunhuang dietarylprolinesupplementationamelioratesautismlikebehaviorsandmodulatesgutmicrobiotainthevalproicacidinducedmousemodelofautismspectrumdisorder AT taotong dietarylprolinesupplementationamelioratesautismlikebehaviorsandmodulatesgutmicrobiotainthevalproicacidinducedmousemodelofautismspectrumdisorder |