Speeding-up Screening of S1 SARS-CoV2 Inhibitors by Nanostructured Biomimetic ACE2 Sensors Based on a Superconductive Josephson Junction Toroidal Array Oscillating Effect
We present a method for integrating a quantum superconductive oscillation effect, induced by a potential S1 SARS-CoV-2 inhibitor, which achieves a 99.992% blockade of S1 virus communication with a biomimetic angiotensin-converting enzyme 2 (ACE2) sensor. The sensitivity comparison indicates a perfor...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
IOP Publishing
2025-01-01
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| Series: | ECS Sensors Plus |
| Subjects: | |
| Online Access: | https://doi.org/10.1149/2754-2726/adbe8c |
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| Summary: | We present a method for integrating a quantum superconductive oscillation effect, induced by a potential S1 SARS-CoV-2 inhibitor, which achieves a 99.992% blockade of S1 virus communication with a biomimetic angiotensin-converting enzyme 2 (ACE2) sensor. The sensitivity comparison indicates a performance of −0.077 μA nM ^−1 with the inhibitor vs −1021 μA nM ^−1 without the inhibitor for S1 concentration from 40 aM to 120 nM under antibody-free and labeling-free conditions. Additionally, Remdesivir has shown a 99.998% blockade of S1 attacks. The device consists of a double-layer superconductive Josephson toroidal junction array (JTJA) membrane, with Friedel oscillation observed in atomic force microscopy (AFM) images. When a potential S1 inhibitor, ABS02, is present, a superconductive quantum oscillation effect is observed in current-voltage (i-V) curves, allowing for high accuracy and sensitivity in the rapid screening of inhibitors. The ACE2 Sensor 1 is configured as Superconductor-Insulator1-Superconductor (S-I _1 -S) combined with I _2 (virus) and S (inhibitor), in comparison to a known S1 inhibitor, Remdesivir. The results from Sensor 1 were compared with those from a native ACE2 Sensor 2, which did not show oscillation in the presence of the inhibitor. Sensor 1 demonstrated significantly higher sensitivity than Sensor 2 in the presence of a virus without an inhibitor. Furthermore, by employing a voltage method, the inhibitors restored 100% of the cells’ reversible membrane potential within a safe range, compared to only 50% restoration without the inhibitors. |
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| ISSN: | 2754-2726 |