ANTIMICROBIAL ACTIVITY OF COMPOUNDS ISOLATED FROM MANGROVE-DERIVED ENDOPHYTIC FUNGUS Actinomucor elegan AGAINST Xanthomonas axonopodis pv. passiflorae

This study investigated bioactive compounds isolated from the mangrove endophytic fungus Actinomucor elegans AcCC18.1A for the control of Xanthomonas axonopodis pv. passiflorae (Xap), the causal agent of bacterial spot disease in passion fruit. Through classical chromatographic techniques, we isolat...

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Main Authors: Kathia Raquel Murillo Padilla, Rayanne Priscila Vieira da Conceição, Gisele da Costa Ramos, Luana Cardoso de Oliveira, José Edson de Sousa Siqueira, Mayra Suelen da Silva Pinheiro, Patrícia Santana Barbosa Marinho, Andrey Moacir do Rosario Marinho
Format: Article
Language:English
Published: Sociedade Brasileira de Química 2025-07-01
Series:Química Nova
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Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422025000600310&lng=en&tlng=en
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author Kathia Raquel Murillo Padilla
Rayanne Priscila Vieira da Conceição
Gisele da Costa Ramos
Luana Cardoso de Oliveira
José Edson de Sousa Siqueira
Mayra Suelen da Silva Pinheiro
Patrícia Santana Barbosa Marinho
Andrey Moacir do Rosario Marinho
author_facet Kathia Raquel Murillo Padilla
Rayanne Priscila Vieira da Conceição
Gisele da Costa Ramos
Luana Cardoso de Oliveira
José Edson de Sousa Siqueira
Mayra Suelen da Silva Pinheiro
Patrícia Santana Barbosa Marinho
Andrey Moacir do Rosario Marinho
author_sort Kathia Raquel Murillo Padilla
collection DOAJ
description This study investigated bioactive compounds isolated from the mangrove endophytic fungus Actinomucor elegans AcCC18.1A for the control of Xanthomonas axonopodis pv. passiflorae (Xap), the causal agent of bacterial spot disease in passion fruit. Through classical chromatographic techniques, we isolated four phenolic compounds: tyrosol (1), 4-hydroxyphenylacetic acid (2), 4-formylphenyl-2-phenylacetate (3), and phenylacetic acid (4), with their structures elucidated through nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS). Among the isolated compounds, compound 3 was characterized as a new natural product. Antimicrobial assays were performed using the broth microdilution method against four Xap strains (Xap1-Xap4), with amoxicillin (3.9 µg mL-1) and tetracycline (3.9 µg mL-1) as positive controls. All compounds exhibited inhibitory activity, with compound 2 showing the highest efficacy (7.8 µg mL-1). These results underscore the biotechnological potential of mangrove endophytic fungi as sources of bioactive molecules against agricultural phytopathogens, offering new insights for developing sustainable strategies to control bacterial spot in passion fruit cultivation.
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publishDate 2025-07-01
publisher Sociedade Brasileira de Química
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spelling doaj-art-4055f1a39c5d41098a0143d3177373bb2025-08-20T02:46:58ZengSociedade Brasileira de QuímicaQuímica Nova1678-70642025-07-0148610.21577/0100-4042.20250153ANTIMICROBIAL ACTIVITY OF COMPOUNDS ISOLATED FROM MANGROVE-DERIVED ENDOPHYTIC FUNGUS Actinomucor elegan AGAINST Xanthomonas axonopodis pv. passifloraeKathia Raquel Murillo PadillaRayanne Priscila Vieira da ConceiçãoGisele da Costa RamosLuana Cardoso de OliveiraJosé Edson de Sousa SiqueiraMayra Suelen da Silva PinheiroPatrícia Santana Barbosa Marinhohttps://orcid.org/0000-0002-9368-8574Andrey Moacir do Rosario Marinhohttps://orcid.org/0000-0002-8981-0995This study investigated bioactive compounds isolated from the mangrove endophytic fungus Actinomucor elegans AcCC18.1A for the control of Xanthomonas axonopodis pv. passiflorae (Xap), the causal agent of bacterial spot disease in passion fruit. Through classical chromatographic techniques, we isolated four phenolic compounds: tyrosol (1), 4-hydroxyphenylacetic acid (2), 4-formylphenyl-2-phenylacetate (3), and phenylacetic acid (4), with their structures elucidated through nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS). Among the isolated compounds, compound 3 was characterized as a new natural product. Antimicrobial assays were performed using the broth microdilution method against four Xap strains (Xap1-Xap4), with amoxicillin (3.9 µg mL-1) and tetracycline (3.9 µg mL-1) as positive controls. All compounds exhibited inhibitory activity, with compound 2 showing the highest efficacy (7.8 µg mL-1). These results underscore the biotechnological potential of mangrove endophytic fungi as sources of bioactive molecules against agricultural phytopathogens, offering new insights for developing sustainable strategies to control bacterial spot in passion fruit cultivation.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422025000600310&lng=en&tlng=enphenolic compoundsXanthomonaspassion fruitbacterial spotActinomucor.
spellingShingle Kathia Raquel Murillo Padilla
Rayanne Priscila Vieira da Conceição
Gisele da Costa Ramos
Luana Cardoso de Oliveira
José Edson de Sousa Siqueira
Mayra Suelen da Silva Pinheiro
Patrícia Santana Barbosa Marinho
Andrey Moacir do Rosario Marinho
ANTIMICROBIAL ACTIVITY OF COMPOUNDS ISOLATED FROM MANGROVE-DERIVED ENDOPHYTIC FUNGUS Actinomucor elegan AGAINST Xanthomonas axonopodis pv. passiflorae
Química Nova
phenolic compounds
Xanthomonas
passion fruit
bacterial spot
Actinomucor.
title ANTIMICROBIAL ACTIVITY OF COMPOUNDS ISOLATED FROM MANGROVE-DERIVED ENDOPHYTIC FUNGUS Actinomucor elegan AGAINST Xanthomonas axonopodis pv. passiflorae
title_full ANTIMICROBIAL ACTIVITY OF COMPOUNDS ISOLATED FROM MANGROVE-DERIVED ENDOPHYTIC FUNGUS Actinomucor elegan AGAINST Xanthomonas axonopodis pv. passiflorae
title_fullStr ANTIMICROBIAL ACTIVITY OF COMPOUNDS ISOLATED FROM MANGROVE-DERIVED ENDOPHYTIC FUNGUS Actinomucor elegan AGAINST Xanthomonas axonopodis pv. passiflorae
title_full_unstemmed ANTIMICROBIAL ACTIVITY OF COMPOUNDS ISOLATED FROM MANGROVE-DERIVED ENDOPHYTIC FUNGUS Actinomucor elegan AGAINST Xanthomonas axonopodis pv. passiflorae
title_short ANTIMICROBIAL ACTIVITY OF COMPOUNDS ISOLATED FROM MANGROVE-DERIVED ENDOPHYTIC FUNGUS Actinomucor elegan AGAINST Xanthomonas axonopodis pv. passiflorae
title_sort antimicrobial activity of compounds isolated from mangrove derived endophytic fungus actinomucor elegan against xanthomonas axonopodis pv passiflorae
topic phenolic compounds
Xanthomonas
passion fruit
bacterial spot
Actinomucor.
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422025000600310&lng=en&tlng=en
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