The Effect of ND: YAG Laser on Microorganisms Causing Dental Caries; In Vitro Study

Purpose: Streptococcus mutans and Lactobacilli are common microorganisms involved in the caries process, while Candida albicans and Streptococcus mutans are linked to higher rates of tooth decay and severe oropharyngeal conditions. Nd: YAG Lasers are utilized in both medicine and dentistry, enhanci...

Full description

Saved in:
Bibliographic Details
Main Authors: Ghada Abdul Salam Ibrahim, Intisar Abdel_Jabbar Al Sarraf, Bushra Hamza Sadik, Safa Thaer Noori, Afnan Akram Mohammed
Format: Article
Language:English
Published: Tehran University of Medical Sciences 2025-07-01
Series:Frontiers in Biomedical Technologies
Subjects:
Online Access:https://fbt.tums.ac.ir/index.php/fbt/article/view/1194
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849393135565668352
author Ghada Abdul Salam Ibrahim
Intisar Abdel_Jabbar Al Sarraf
Bushra Hamza Sadik
Safa Thaer Noori
Afnan Akram Mohammed
author_facet Ghada Abdul Salam Ibrahim
Intisar Abdel_Jabbar Al Sarraf
Bushra Hamza Sadik
Safa Thaer Noori
Afnan Akram Mohammed
author_sort Ghada Abdul Salam Ibrahim
collection DOAJ
description Purpose: Streptococcus mutans and Lactobacilli are common microorganisms involved in the caries process, while Candida albicans and Streptococcus mutans are linked to higher rates of tooth decay and severe oropharyngeal conditions. Nd: YAG Lasers are utilized in both medicine and dentistry, enhancing dental enamel's acid resistance and harming pathogenic organisms. This study aims to assess the sensitivity of Candida albicans and Streptococcus mutans to Nd: YAG Laser treatment. Materials and Methods: Microorganisms were exposed to a Q-switched Nd: YAG Laser with a wavelength of 1064 nm, a beam diameter of 4 mm, and energy levels of 100 and 200 mJ for S. mutans, and 300 and 400 mJ for C. albicans. The laser emitted 100 and 200 pulses at a repetition frequency of 3 Hz. All data underwent statistical analysis. Results: Exposing the Streptococcus mutans and Candida albicans to neodymium dopped-yettrium aluminum garnet, resulted in a reduction in colony forming unit/milliliter (CFU/mL) with increased Laser energy and numbers of pulse with maximum reduction at 200 mJ – 200 pulse and 400 mJ – 200 pulse for Streptococcus mutans and Candida albicans, respectively. A statistically significant differences were shown in the bacterial number (CFU/mL) between each group when compared with the control group. A statistically significant differences were shown in the fungal number (CFU/mL) between each group in comparison with the control group except between 300mJ-100 pulse and control, there is no significant difference. Conclusion: ND: YAG exhibits antimicrobial effects against Candida albicans and Streptococcus mutans, with a stronger effect on the latter. Additionally, ND: YAG laser therapy promotes wound healing and reduces inflammation, making it an effective tool for managing oral infections. Its deep tissue penetration enables targeted treatment while minimizing harm to surrounding healthy cells. The principle of selective photothermolysis, where the laser energy is preferentially absorbed by pathogens, further enhances its efficacy against these microorganisms.
format Article
id doaj-art-4050d93c8d95453ca4b988e3ec1523a4
institution Kabale University
issn 2345-5837
language English
publishDate 2025-07-01
publisher Tehran University of Medical Sciences
record_format Article
series Frontiers in Biomedical Technologies
spelling doaj-art-4050d93c8d95453ca4b988e3ec1523a42025-08-20T03:40:33ZengTehran University of Medical SciencesFrontiers in Biomedical Technologies2345-58372025-07-0112310.18502/fbt.v12i3.19172The Effect of ND: YAG Laser on Microorganisms Causing Dental Caries; In Vitro StudyGhada Abdul Salam Ibrahim0Intisar Abdel_Jabbar Al Sarraf1Bushra Hamza Sadik2Safa Thaer Noori3Afnan Akram Mohammed4Ph.D preventive dentistry, Osol AL-ELM University Collage- Department of Dentistry, Baghdad, Iraq 1 Al- Baladiyat Specialized Dental Centre, ministry of health, Baghdad, IraqPreventive Dentistry, Osol AL-ELM University Collage, Department of Dentistry, Baghdad, IraqPreventive Dentistry, Osol AL-ELM University Collage, Department of Dentistry, Baghdad, IraqPreventive Dentistry, Osol AL-ELM University Collage, Department of Dentistry, Baghdad, IraqPreventive Dentistry, Osol AL-ELM University Collage, Department of Dentistry, Baghdad, Iraq Purpose: Streptococcus mutans and Lactobacilli are common microorganisms involved in the caries process, while Candida albicans and Streptococcus mutans are linked to higher rates of tooth decay and severe oropharyngeal conditions. Nd: YAG Lasers are utilized in both medicine and dentistry, enhancing dental enamel's acid resistance and harming pathogenic organisms. This study aims to assess the sensitivity of Candida albicans and Streptococcus mutans to Nd: YAG Laser treatment. Materials and Methods: Microorganisms were exposed to a Q-switched Nd: YAG Laser with a wavelength of 1064 nm, a beam diameter of 4 mm, and energy levels of 100 and 200 mJ for S. mutans, and 300 and 400 mJ for C. albicans. The laser emitted 100 and 200 pulses at a repetition frequency of 3 Hz. All data underwent statistical analysis. Results: Exposing the Streptococcus mutans and Candida albicans to neodymium dopped-yettrium aluminum garnet, resulted in a reduction in colony forming unit/milliliter (CFU/mL) with increased Laser energy and numbers of pulse with maximum reduction at 200 mJ – 200 pulse and 400 mJ – 200 pulse for Streptococcus mutans and Candida albicans, respectively. A statistically significant differences were shown in the bacterial number (CFU/mL) between each group when compared with the control group. A statistically significant differences were shown in the fungal number (CFU/mL) between each group in comparison with the control group except between 300mJ-100 pulse and control, there is no significant difference. Conclusion: ND: YAG exhibits antimicrobial effects against Candida albicans and Streptococcus mutans, with a stronger effect on the latter. Additionally, ND: YAG laser therapy promotes wound healing and reduces inflammation, making it an effective tool for managing oral infections. Its deep tissue penetration enables targeted treatment while minimizing harm to surrounding healthy cells. The principle of selective photothermolysis, where the laser energy is preferentially absorbed by pathogens, further enhances its efficacy against these microorganisms. https://fbt.tums.ac.ir/index.php/fbt/article/view/1194ND: YAG LaserDental CariesMicroorganismsCandida Albicans
spellingShingle Ghada Abdul Salam Ibrahim
Intisar Abdel_Jabbar Al Sarraf
Bushra Hamza Sadik
Safa Thaer Noori
Afnan Akram Mohammed
The Effect of ND: YAG Laser on Microorganisms Causing Dental Caries; In Vitro Study
Frontiers in Biomedical Technologies
ND: YAG Laser
Dental Caries
Microorganisms
Candida Albicans
title The Effect of ND: YAG Laser on Microorganisms Causing Dental Caries; In Vitro Study
title_full The Effect of ND: YAG Laser on Microorganisms Causing Dental Caries; In Vitro Study
title_fullStr The Effect of ND: YAG Laser on Microorganisms Causing Dental Caries; In Vitro Study
title_full_unstemmed The Effect of ND: YAG Laser on Microorganisms Causing Dental Caries; In Vitro Study
title_short The Effect of ND: YAG Laser on Microorganisms Causing Dental Caries; In Vitro Study
title_sort effect of nd yag laser on microorganisms causing dental caries in vitro study
topic ND: YAG Laser
Dental Caries
Microorganisms
Candida Albicans
url https://fbt.tums.ac.ir/index.php/fbt/article/view/1194
work_keys_str_mv AT ghadaabdulsalamibrahim theeffectofndyaglaseronmicroorganismscausingdentalcariesinvitrostudy
AT intisarabdeljabbaralsarraf theeffectofndyaglaseronmicroorganismscausingdentalcariesinvitrostudy
AT bushrahamzasadik theeffectofndyaglaseronmicroorganismscausingdentalcariesinvitrostudy
AT safathaernoori theeffectofndyaglaseronmicroorganismscausingdentalcariesinvitrostudy
AT afnanakrammohammed theeffectofndyaglaseronmicroorganismscausingdentalcariesinvitrostudy
AT ghadaabdulsalamibrahim effectofndyaglaseronmicroorganismscausingdentalcariesinvitrostudy
AT intisarabdeljabbaralsarraf effectofndyaglaseronmicroorganismscausingdentalcariesinvitrostudy
AT bushrahamzasadik effectofndyaglaseronmicroorganismscausingdentalcariesinvitrostudy
AT safathaernoori effectofndyaglaseronmicroorganismscausingdentalcariesinvitrostudy
AT afnanakrammohammed effectofndyaglaseronmicroorganismscausingdentalcariesinvitrostudy