Simultaneous identification of multi-genes with resistance to root-knot nematode and tomato leaf mold by PCR reaction in tomato
Mi-1 and Cf-9 genes in tomato tightly linked with two CAPS (cleaved amplified polymorphic sequence) markers and respectively resistant to root-knot nematode and leaf mold were amplified and screened by using a single PCR reaction. The PCR products were correspond to the amplified bands produced by s...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2008-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.02.007 |
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| Summary: | Mi-1 and Cf-9 genes in tomato tightly linked with two CAPS (cleaved amplified polymorphic sequence) markers and respectively resistant to root-knot nematode and leaf mold were amplified and screened by using a single PCR reaction. The PCR products were correspond to the amplified bands produced by single CAPS primer. Among them, 2775 bp fragment linked with Cf-9 gene was amplified in the resistant tomato lines. The amplified bands were distinguishable after cleavage with the restriction enzyme Taq Ⅰ. Genotype with Cf-9 gene could produce 1177 bp, 446 bp, 370 bp and 160 bp bands, respectively. The co-dominant CAPS2 marker tightly linked with Mi-1 gene produced 915 bp fragment in the resistant tomato lines. Genotype with Mi-1 gene could produce 719 bp and 196 bp bands, respectively. The replicated stable results proved that two resistant genes could be identified simultaneously by using corresponding CAPS primer under adaptable condition. Compared with single primer PCR, this system is time-saving, labor-saving and low cost. It could be very useful for marker-assisted selection during early stage in tomato and efficiently speed up breeding procedure. |
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| ISSN: | 1008-9209 2097-5155 |