Oncolytic vaccinia virus harboring CLEC2A gene enhances viral replication and antitumor efficacy
In the field of innovative cancer treatment strategies, oncolytic vaccinia virus (VV)es have gained traction as promising vectors. In the current study, we inserted the human C-type lectin domain family 2 member A (CLEC2A) gene into VV, creating a replicating therapeutic, oncoVV-CLEC2A. The findings...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-09-01
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| Series: | Molecular Therapy: Oncology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2950329924000651 |
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| author | Chunqing Gao Qi Ying Yufeng Qiu Ningbo Ren Kan Chen Yanrong Zhou Ting Ye Gongchu Li |
| author_facet | Chunqing Gao Qi Ying Yufeng Qiu Ningbo Ren Kan Chen Yanrong Zhou Ting Ye Gongchu Li |
| author_sort | Chunqing Gao |
| collection | DOAJ |
| description | In the field of innovative cancer treatment strategies, oncolytic vaccinia virus (VV)es have gained traction as promising vectors. In the current study, we inserted the human C-type lectin domain family 2 member A (CLEC2A) gene into VV, creating a replicating therapeutic, oncoVV-CLEC2A. The findings reveal that oncoVV-CLEC2A effectively suppresses colorectal proliferation of mouse xenografts and a range of human cancer cell lines by augmenting viral reproduction capabilities, including the lung cancer H460 cell line, colorectal cancer cell lines (HCT116 and SW620), and hepatocellular carcinoma HuH-7 cell line. Moreover, it is evident that oncoVV-CLEC2A can induce antitumor immunity by boosting cytokine production but not antivirus response, and enhancing calreticulin expression. Further investigation indicates that oncoVV-CLEC2A can enhance antitumor capabilities by activating natural killer cells to produce interferon-γ and induce M1-like macrophage polarization. These findings shed light on the antitumor mechanisms of oncoVV-CLEC2A, provide a theoretical basis for oncolytic therapies, and lay the groundwork for novel strategies for modifying VVs. |
| format | Article |
| id | doaj-art-4046597af92747dbafb8310fc3d7cae0 |
| institution | OA Journals |
| issn | 2950-3299 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Therapy: Oncology |
| spelling | doaj-art-4046597af92747dbafb8310fc3d7cae02025-08-20T02:22:32ZengElsevierMolecular Therapy: Oncology2950-32992024-09-0132320082310.1016/j.omton.2024.200823Oncolytic vaccinia virus harboring CLEC2A gene enhances viral replication and antitumor efficacyChunqing Gao0Qi Ying1Yufeng Qiu2Ningbo Ren3Kan Chen4Yanrong Zhou5Ting Ye6Gongchu Li7College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China; Corresponding author: Ting Ye, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China.College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China; Corresponding author: Gongchu Li, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China.In the field of innovative cancer treatment strategies, oncolytic vaccinia virus (VV)es have gained traction as promising vectors. In the current study, we inserted the human C-type lectin domain family 2 member A (CLEC2A) gene into VV, creating a replicating therapeutic, oncoVV-CLEC2A. The findings reveal that oncoVV-CLEC2A effectively suppresses colorectal proliferation of mouse xenografts and a range of human cancer cell lines by augmenting viral reproduction capabilities, including the lung cancer H460 cell line, colorectal cancer cell lines (HCT116 and SW620), and hepatocellular carcinoma HuH-7 cell line. Moreover, it is evident that oncoVV-CLEC2A can induce antitumor immunity by boosting cytokine production but not antivirus response, and enhancing calreticulin expression. Further investigation indicates that oncoVV-CLEC2A can enhance antitumor capabilities by activating natural killer cells to produce interferon-γ and induce M1-like macrophage polarization. These findings shed light on the antitumor mechanisms of oncoVV-CLEC2A, provide a theoretical basis for oncolytic therapies, and lay the groundwork for novel strategies for modifying VVs.http://www.sciencedirect.com/science/article/pii/S2950329924000651MT: Regular IssueC-type lectin domain family 2 member Aoncolytic vaccinia virusantitumorviral replicationNK cells |
| spellingShingle | Chunqing Gao Qi Ying Yufeng Qiu Ningbo Ren Kan Chen Yanrong Zhou Ting Ye Gongchu Li Oncolytic vaccinia virus harboring CLEC2A gene enhances viral replication and antitumor efficacy Molecular Therapy: Oncology MT: Regular Issue C-type lectin domain family 2 member A oncolytic vaccinia virus antitumor viral replication NK cells |
| title | Oncolytic vaccinia virus harboring CLEC2A gene enhances viral replication and antitumor efficacy |
| title_full | Oncolytic vaccinia virus harboring CLEC2A gene enhances viral replication and antitumor efficacy |
| title_fullStr | Oncolytic vaccinia virus harboring CLEC2A gene enhances viral replication and antitumor efficacy |
| title_full_unstemmed | Oncolytic vaccinia virus harboring CLEC2A gene enhances viral replication and antitumor efficacy |
| title_short | Oncolytic vaccinia virus harboring CLEC2A gene enhances viral replication and antitumor efficacy |
| title_sort | oncolytic vaccinia virus harboring clec2a gene enhances viral replication and antitumor efficacy |
| topic | MT: Regular Issue C-type lectin domain family 2 member A oncolytic vaccinia virus antitumor viral replication NK cells |
| url | http://www.sciencedirect.com/science/article/pii/S2950329924000651 |
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