EXPERIMENTAL REPAIR OF DEEP CORNEAL DEFECTS USING A BIO-CONSTRUCT COMPRISING A COLLAGEN TYPE I MATRIX LOADED WITH BUCCAL EPITHELIAL CELLS
The research objective was to study the reparative effects of the collagen type I bio-construct loaded with buccal epithelial cells, on the rabbit cornea after experimental keratectomy at various stages of treatment (on the 3rd, 7th, 14th, 3 0th days).Material and methods. The experiment...
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| Language: | English |
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N.V. Sklifosovsky Research Institute for Emergency Medicine of Moscow Healthcare Department
2017-09-01
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| Series: | Трансплантология (Москва) |
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| Online Access: | https://www.jtransplantologiya.ru/jour/article/view/180 |
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| author | N. S. Egorova E. V. Chentsova N. V. Borovkova M. S. Makarov M. V. Storozheva |
| author_facet | N. S. Egorova E. V. Chentsova N. V. Borovkova M. S. Makarov M. V. Storozheva |
| author_sort | N. S. Egorova |
| collection | DOAJ |
| description | The research objective was to study the reparative effects of the collagen type I bio-construct loaded with buccal epithelial cells, on the rabbit cornea after experimental keratectomy at various stages of treatment (on the 3rd, 7th, 14th, 3 0th days).Material and methods. The experiments were conducted on 20 rabbits of the Chinchilla breed that were operated on cornea of both eyes aiming to inflict epithelial and stromal cornea defects. The collagen-based bio-construct bearing buccal epithelial cells was placed over the cornea of the experimental eyes. The cornea of the control eyes was covered with smooth contact lens. After the surgery, a temporal blepharorrhaphy was performed and kept for 3 days. We studied macroand microscopic pattern of corneal regeneration at 3, 7, 14, and 30 days of experiment.Results. When using the collaged-based bio-construct containing buccal epithelial cells, the complete epithelialization of the corneal defect occurred at mean 7 days earlier compared to that in the control eyes. Thus, the offered bio-construct stimulated the cell migration and proliferation at early stages of treatment (3–7 days) reducing the inflammation activity.Conclusion. The bio-construct comprising a collagen type I matrix loaded with buccal epithelial cells can provide an effective treatment option for corneal defects. |
| format | Article |
| id | doaj-art-40441d4662c84ffdbd2202931c2542c4 |
| institution | DOAJ |
| issn | 2074-0506 2542-0909 |
| language | English |
| publishDate | 2017-09-01 |
| publisher | N.V. Sklifosovsky Research Institute for Emergency Medicine of Moscow Healthcare Department |
| record_format | Article |
| series | Трансплантология (Москва) |
| spelling | doaj-art-40441d4662c84ffdbd2202931c2542c42025-08-20T02:55:45ZengN.V. Sklifosovsky Research Institute for Emergency Medicine of Moscow Healthcare DepartmentТрансплантология (Москва)2074-05062542-09092017-09-019322623510.23873/2074-0506-2017-9-3-226-235174EXPERIMENTAL REPAIR OF DEEP CORNEAL DEFECTS USING A BIO-CONSTRUCT COMPRISING A COLLAGEN TYPE I MATRIX LOADED WITH BUCCAL EPITHELIAL CELLSN. S. Egorova0E. V. Chentsova1N. V. Borovkova2M. S. Makarov3M. V. Storozheva4Moscow Helmholtz’s Research Institute of Eye DiseasesMoscow Helmholtz’s Research Institute of Eye DiseasesN.V. Sklifosovsky Research Institute for Emergency MedicineN.V. Sklifosovsky Research Institute for Emergency MedicineN.V. Sklifosovsky Research Institute for Emergency MedicineThe research objective was to study the reparative effects of the collagen type I bio-construct loaded with buccal epithelial cells, on the rabbit cornea after experimental keratectomy at various stages of treatment (on the 3rd, 7th, 14th, 3 0th days).Material and methods. The experiments were conducted on 20 rabbits of the Chinchilla breed that were operated on cornea of both eyes aiming to inflict epithelial and stromal cornea defects. The collagen-based bio-construct bearing buccal epithelial cells was placed over the cornea of the experimental eyes. The cornea of the control eyes was covered with smooth contact lens. After the surgery, a temporal blepharorrhaphy was performed and kept for 3 days. We studied macroand microscopic pattern of corneal regeneration at 3, 7, 14, and 30 days of experiment.Results. When using the collaged-based bio-construct containing buccal epithelial cells, the complete epithelialization of the corneal defect occurred at mean 7 days earlier compared to that in the control eyes. Thus, the offered bio-construct stimulated the cell migration and proliferation at early stages of treatment (3–7 days) reducing the inflammation activity.Conclusion. The bio-construct comprising a collagen type I matrix loaded with buccal epithelial cells can provide an effective treatment option for corneal defects.https://www.jtransplantologiya.ru/jour/article/view/180corneal defectexperimental studybuccal epitheliumcollagenbio-construct |
| spellingShingle | N. S. Egorova E. V. Chentsova N. V. Borovkova M. S. Makarov M. V. Storozheva EXPERIMENTAL REPAIR OF DEEP CORNEAL DEFECTS USING A BIO-CONSTRUCT COMPRISING A COLLAGEN TYPE I MATRIX LOADED WITH BUCCAL EPITHELIAL CELLS Трансплантология (Москва) corneal defect experimental study buccal epithelium collagen bio-construct |
| title | EXPERIMENTAL REPAIR OF DEEP CORNEAL DEFECTS USING A BIO-CONSTRUCT COMPRISING A COLLAGEN TYPE I MATRIX LOADED WITH BUCCAL EPITHELIAL CELLS |
| title_full | EXPERIMENTAL REPAIR OF DEEP CORNEAL DEFECTS USING A BIO-CONSTRUCT COMPRISING A COLLAGEN TYPE I MATRIX LOADED WITH BUCCAL EPITHELIAL CELLS |
| title_fullStr | EXPERIMENTAL REPAIR OF DEEP CORNEAL DEFECTS USING A BIO-CONSTRUCT COMPRISING A COLLAGEN TYPE I MATRIX LOADED WITH BUCCAL EPITHELIAL CELLS |
| title_full_unstemmed | EXPERIMENTAL REPAIR OF DEEP CORNEAL DEFECTS USING A BIO-CONSTRUCT COMPRISING A COLLAGEN TYPE I MATRIX LOADED WITH BUCCAL EPITHELIAL CELLS |
| title_short | EXPERIMENTAL REPAIR OF DEEP CORNEAL DEFECTS USING A BIO-CONSTRUCT COMPRISING A COLLAGEN TYPE I MATRIX LOADED WITH BUCCAL EPITHELIAL CELLS |
| title_sort | experimental repair of deep corneal defects using a bio construct comprising a collagen type i matrix loaded with buccal epithelial cells |
| topic | corneal defect experimental study buccal epithelium collagen bio-construct |
| url | https://www.jtransplantologiya.ru/jour/article/view/180 |
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