Synthesis, Characterization, and Evaluation of a Novel Amphiphilic Polymer RGD-PEG-Chol for Target Drug Delivery System
An amphiphilic polymer RGD-PEG-Chol which can be produced in large scale at a very low cost has been synthesized successfully. The synthesized intermediates and final products were characterized and confirmed by 1H nuclear magnetic resonance spectrum (1H NMR) and Fourier transform infrared spectrum...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/546176 |
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| _version_ | 1850105052595748864 |
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| author | Shi Zeng Fengbo Wu Bo Li Xiangrong Song Yu Zheng Gu He Cheng Peng Wei Huang |
| author_facet | Shi Zeng Fengbo Wu Bo Li Xiangrong Song Yu Zheng Gu He Cheng Peng Wei Huang |
| author_sort | Shi Zeng |
| collection | DOAJ |
| description | An amphiphilic polymer RGD-PEG-Chol which can be produced in large scale at a very low cost has been synthesized successfully. The synthesized intermediates and final products were characterized and confirmed by 1H nuclear magnetic resonance spectrum (1H NMR) and Fourier transform infrared spectrum (FT-IR). The paclitaxel- (PTX-) loaded liposomes based on RGD-PEG-Chol were then prepared by film formation method. The liposomes had a size within 100 nm and significantly enhanced the cytotoxicity of paclitaxel to B16F10 cell as demonstrated by MTT test (IC50 = 0.079 μg/mL of RGD-modified PTX-loaded liposomes compared to 9.57 μg/mL of free PTX). Flow cytometry analysis revealed that the cellular uptake of coumarin encapsulated in the RGD-PEG-Chol modified liposome was increased for HUVEC cells. This work provides a reasonable, facile, and economic approach to prepare peptide-modified liposome materials with controllable performances and the obtained linear RGD-modified PTX-loaded liposomes might be attractive as a drug delivery system. |
| format | Article |
| id | doaj-art-403371ff5bd640218feb077375dfc470 |
| institution | DOAJ |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-403371ff5bd640218feb077375dfc4702025-08-20T02:39:12ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/546176546176Synthesis, Characterization, and Evaluation of a Novel Amphiphilic Polymer RGD-PEG-Chol for Target Drug Delivery SystemShi Zeng0Fengbo Wu1Bo Li2Xiangrong Song3Yu Zheng4Gu He5Cheng Peng6Wei Huang7State Key Laboratory of Biotherapy and Department of Pharmacy, West China Hospital, Sichuan University, No. 37 Guoxue Alley, Chengdu, Sichuan 610041, ChinaState Key Laboratory of Biotherapy and Department of Pharmacy, West China Hospital, Sichuan University, No. 37 Guoxue Alley, Chengdu, Sichuan 610041, ChinaState Key Laboratory of Biotherapy and Department of Pharmacy, West China Hospital, Sichuan University, No. 37 Guoxue Alley, Chengdu, Sichuan 610041, ChinaState Key Laboratory of Biotherapy and Department of Pharmacy, West China Hospital, Sichuan University, No. 37 Guoxue Alley, Chengdu, Sichuan 610041, ChinaState Key Laboratory of Biotherapy and Department of Pharmacy, West China Hospital, Sichuan University, No. 37 Guoxue Alley, Chengdu, Sichuan 610041, ChinaState Key Laboratory of Biotherapy and Department of Pharmacy, West China Hospital, Sichuan University, No. 37 Guoxue Alley, Chengdu, Sichuan 610041, ChinaState Key Laboratory Breeding Base of Systematic research, Development and Utilization of Chinese Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaState Key Laboratory Breeding Base of Systematic research, Development and Utilization of Chinese Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaAn amphiphilic polymer RGD-PEG-Chol which can be produced in large scale at a very low cost has been synthesized successfully. The synthesized intermediates and final products were characterized and confirmed by 1H nuclear magnetic resonance spectrum (1H NMR) and Fourier transform infrared spectrum (FT-IR). The paclitaxel- (PTX-) loaded liposomes based on RGD-PEG-Chol were then prepared by film formation method. The liposomes had a size within 100 nm and significantly enhanced the cytotoxicity of paclitaxel to B16F10 cell as demonstrated by MTT test (IC50 = 0.079 μg/mL of RGD-modified PTX-loaded liposomes compared to 9.57 μg/mL of free PTX). Flow cytometry analysis revealed that the cellular uptake of coumarin encapsulated in the RGD-PEG-Chol modified liposome was increased for HUVEC cells. This work provides a reasonable, facile, and economic approach to prepare peptide-modified liposome materials with controllable performances and the obtained linear RGD-modified PTX-loaded liposomes might be attractive as a drug delivery system.http://dx.doi.org/10.1155/2014/546176 |
| spellingShingle | Shi Zeng Fengbo Wu Bo Li Xiangrong Song Yu Zheng Gu He Cheng Peng Wei Huang Synthesis, Characterization, and Evaluation of a Novel Amphiphilic Polymer RGD-PEG-Chol for Target Drug Delivery System The Scientific World Journal |
| title | Synthesis, Characterization, and Evaluation of a Novel Amphiphilic Polymer RGD-PEG-Chol for Target Drug Delivery System |
| title_full | Synthesis, Characterization, and Evaluation of a Novel Amphiphilic Polymer RGD-PEG-Chol for Target Drug Delivery System |
| title_fullStr | Synthesis, Characterization, and Evaluation of a Novel Amphiphilic Polymer RGD-PEG-Chol for Target Drug Delivery System |
| title_full_unstemmed | Synthesis, Characterization, and Evaluation of a Novel Amphiphilic Polymer RGD-PEG-Chol for Target Drug Delivery System |
| title_short | Synthesis, Characterization, and Evaluation of a Novel Amphiphilic Polymer RGD-PEG-Chol for Target Drug Delivery System |
| title_sort | synthesis characterization and evaluation of a novel amphiphilic polymer rgd peg chol for target drug delivery system |
| url | http://dx.doi.org/10.1155/2014/546176 |
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