Morphologic, Molecular and Pathogenic Characterization of Phomopsis viticola isolates Collected from the Vineyards of the Aegean Region in Turkey
Phomopsis cane and leaf spot caused by Phomopsis viticola (dead arm) is an important problem in the vineyards of the Aegean Region of Turkey, like in many parts of the world. In this study, morphological molecular and pathogenic characteristics of P. viticola isolates collected from the vineyards of...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Yuzuncu Yil University Faculty of Agriculture
2021-06-01
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| Series: | Yüzüncü Yıl Üniversitesi Tarim Bilimleri Dergisi |
| Subjects: | |
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| Summary: | Phomopsis cane and leaf spot caused by Phomopsis viticola (dead arm) is an important problem in the vineyards of the Aegean Region of Turkey, like in many parts of the world. In this study, morphological molecular and pathogenic characteristics of P. viticola isolates collected from the vineyards of the Aegean Region were determined. For this purpose, 416 shoot samples were collected and a total of 232 Phomopsis spp isolates were isolated. In the majority of isolates (94.39%), only alpha conidia were observed, while alpha and beta conidia ocured in only 13 isolates. While most of the isolates (69.39%) showed rapid colony growth, a limited number of isolates (15 izolates) produced slow growing colony. In the fungal colony colour characteristics, 35.77% of the isolates were white, 59.05% were gray and 5.17% were light cream. In the PCR assay, 214 isolates were identified as P. viticola. No important differences were found between PCR negative and PCR positive isolates morphologically. Therefore, molecular diagnosis must be made in order to correctly identify P. viticola. This study indicated that most of the isolates (92.24%) obtained from the plants showing Phomopsis cane and leaf spot symptoms in vineyards of the Aegean Region are P. viticola. There were different levels of virulence with the lesion lenght ranging between 2.3 and 18.7 cm among the isolates tested in pathogenicity test; however, no relationships were observed between any of the morphological and virulence level. |
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| ISSN: | 1308-7576 1308-7584 |