Comparison of Leptin and Tnf-Α Levels in Type 2 Diabetes Mellitus Patients with and Without Obesity

Background: Leptin and tumor necrosis factor alpha (TNF-α) are the main adipokine products of adipose tissue, leptin is a hormone that controls appetite, while TNF-α acts as a proinflammatory cytokine that can cause Insulin resistance Leptin and TNF-α levels are influenced by body mass index (BMI)....

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Main Authors: Abdulloh ABBAS, Lisyani B. SUROMO, Meita HENDRIANINGTYAS, Nyoman Suci WIDYASTIT, Dwi RETNONINGRUM, Luluk CAHYANTI
Format: Article
Language:English
Published: Bucharest College of Physicians 2024-12-01
Series:Modern Medicine
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Online Access:https://medicinamoderna.ro/wp-content/uploads/2024/12/Comparison-of-Leptin-and-Tnf-%CE%91-Levels-in-Type-2-Diabetes-Mellitus-Patients-with-and-Without-Obesity.pdf
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author Abdulloh ABBAS
Lisyani B. SUROMO
Meita HENDRIANINGTYAS
Nyoman Suci WIDYASTIT
Dwi RETNONINGRUM
Luluk CAHYANTI
author_facet Abdulloh ABBAS
Lisyani B. SUROMO
Meita HENDRIANINGTYAS
Nyoman Suci WIDYASTIT
Dwi RETNONINGRUM
Luluk CAHYANTI
author_sort Abdulloh ABBAS
collection DOAJ
description Background: Leptin and tumor necrosis factor alpha (TNF-α) are the main adipokine products of adipose tissue, leptin is a hormone that controls appetite, while TNF-α acts as a proinflammatory cytokine that can cause Insulin resistance Leptin and TNF-α levels are influenced by body mass index (BMI). Objective: To prove the differences in leptin and TNF-α levels between T2DM patients with and without obesity. Material and Method: A cross-sectional study was conducted on 31 T2DM subjects with obesity and 29 DMT2 subjects without obesity. Leptin and TNF-α levels were measured using the enzyme linked immunosorbent assay (ELISA) method. Obesity status was determined based on BMI values where BMI ≥25 kg / m2 was the obesity group and BMI <25 kg / m2 group without obesity. The t-test was used as the comparative test. Results: The median leptin levels in the obese and non-obese T2DM groups were 31.8(7;74.6) ng/mL and 9.2(0.8;36.7) ng/mL. There was a difference in leptin levels between the two groups with p=0.001. The median TNF-α levels in the obese and non-obese DMT2 group were 6.6(2.2;21.2) pg/mL, and 4.4(1.6;15.8) pg/mL. There was a difference in TNF-α levels between the two groups with p=0.007. Conclusion: There are significant differences in leptin and TNF-α levels between T2DM patients with and without obesity, where leptin and TNF-α levels are higher in T2DM patients with obesity than T2DM patients without obesity.
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spelling doaj-art-4009a1db6d4b4eac83707a4e0562edab2025-08-20T02:53:37ZengBucharest College of PhysiciansModern Medicine1223-04722360-24732024-12-0131432533010.31689/rmm.2024.31.4.325Comparison of Leptin and Tnf-Α Levels in Type 2 Diabetes Mellitus Patients with and Without ObesityAbdulloh ABBAS0Lisyani B. SUROMO1https://orcid.org/0009-0004-2107-847XMeita HENDRIANINGTYAS2https://orcid.org/0000-0003-1554-2876Nyoman Suci WIDYASTIT3Dwi RETNONINGRUM4Luluk CAHYANTI5 Clinical Pathology Faculty of Medicine Diponegoro University Semarang, IndonesiaClinical Pathology Faculty of Medicine Diponegoro University Semarang, IndonesiaClinical Pathology Faculty of Medicine Diponegoro University Semarang, IndonesiaClinical Pathology Faculty of Medicine Diponegoro University Semarang, IndonesiaClinical Pathology Faculty of Medicine Diponegoro University Semarang, Indonesia AKPER Krida Husada Kudus, IndonesiaBackground: Leptin and tumor necrosis factor alpha (TNF-α) are the main adipokine products of adipose tissue, leptin is a hormone that controls appetite, while TNF-α acts as a proinflammatory cytokine that can cause Insulin resistance Leptin and TNF-α levels are influenced by body mass index (BMI). Objective: To prove the differences in leptin and TNF-α levels between T2DM patients with and without obesity. Material and Method: A cross-sectional study was conducted on 31 T2DM subjects with obesity and 29 DMT2 subjects without obesity. Leptin and TNF-α levels were measured using the enzyme linked immunosorbent assay (ELISA) method. Obesity status was determined based on BMI values where BMI ≥25 kg / m2 was the obesity group and BMI <25 kg / m2 group without obesity. The t-test was used as the comparative test. Results: The median leptin levels in the obese and non-obese T2DM groups were 31.8(7;74.6) ng/mL and 9.2(0.8;36.7) ng/mL. There was a difference in leptin levels between the two groups with p=0.001. The median TNF-α levels in the obese and non-obese DMT2 group were 6.6(2.2;21.2) pg/mL, and 4.4(1.6;15.8) pg/mL. There was a difference in TNF-α levels between the two groups with p=0.007. Conclusion: There are significant differences in leptin and TNF-α levels between T2DM patients with and without obesity, where leptin and TNF-α levels are higher in T2DM patients with obesity than T2DM patients without obesity.https://medicinamoderna.ro/wp-content/uploads/2024/12/Comparison-of-Leptin-and-Tnf-%CE%91-Levels-in-Type-2-Diabetes-Mellitus-Patients-with-and-Without-Obesity.pdfdiabetes mellitus type-2leptintnf-αobesity
spellingShingle Abdulloh ABBAS
Lisyani B. SUROMO
Meita HENDRIANINGTYAS
Nyoman Suci WIDYASTIT
Dwi RETNONINGRUM
Luluk CAHYANTI
Comparison of Leptin and Tnf-Α Levels in Type 2 Diabetes Mellitus Patients with and Without Obesity
Modern Medicine
diabetes mellitus type-2
leptin
tnf-α
obesity
title Comparison of Leptin and Tnf-Α Levels in Type 2 Diabetes Mellitus Patients with and Without Obesity
title_full Comparison of Leptin and Tnf-Α Levels in Type 2 Diabetes Mellitus Patients with and Without Obesity
title_fullStr Comparison of Leptin and Tnf-Α Levels in Type 2 Diabetes Mellitus Patients with and Without Obesity
title_full_unstemmed Comparison of Leptin and Tnf-Α Levels in Type 2 Diabetes Mellitus Patients with and Without Obesity
title_short Comparison of Leptin and Tnf-Α Levels in Type 2 Diabetes Mellitus Patients with and Without Obesity
title_sort comparison of leptin and tnf α levels in type 2 diabetes mellitus patients with and without obesity
topic diabetes mellitus type-2
leptin
tnf-α
obesity
url https://medicinamoderna.ro/wp-content/uploads/2024/12/Comparison-of-Leptin-and-Tnf-%CE%91-Levels-in-Type-2-Diabetes-Mellitus-Patients-with-and-Without-Obesity.pdf
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