Evolution of the Neopsylla hongyangensis Mitogenome: Insights Into the Mitogenomic Evolution of the Orders Siphonaptera and the Phthiraptera

ABSTRACT The evidence that parasitic animals exhibit elevated mitogenomic evolution rates is inconsistent and limited to Arthropoda. Neopsylla hongyangensis Li, Bai et Chen, 1986 (Siphonaptera: Ctenophthalmidae) feeds on the host's blood and is an important medical insect with plague transmissi...

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Main Authors: Xiaoxia Lin, Ju Pu, Wenge Dong
Format: Article
Language:English
Published: Wiley 2025-03-01
Series:Ecology and Evolution
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Online Access:https://doi.org/10.1002/ece3.71108
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author Xiaoxia Lin
Ju Pu
Wenge Dong
author_facet Xiaoxia Lin
Ju Pu
Wenge Dong
author_sort Xiaoxia Lin
collection DOAJ
description ABSTRACT The evidence that parasitic animals exhibit elevated mitogenomic evolution rates is inconsistent and limited to Arthropoda. Neopsylla hongyangensis Li, Bai et Chen, 1986 (Siphonaptera: Ctenophthalmidae) feeds on the host's blood and is an important medical insect with plague transmission. In this study, we sequenced the N. hongyangensis mitogenome and explored the mitogenomic evolution of Siphonaptera and Phthiraptera, which both belong to the Insecta on warm‐blooded animals. The mitogenomes of Siphonaptera are closed‐circular double‐stranded DNA molecules and exhibit highly conserved structural features. In contrast, the mitogenomes of most Phthiraptera species exhibit extensive fragmentation and comprise multiple minichromosomes. We performed a comparative analysis of nucleotide composition, Ka/Ks ratios, and codon usage patterns in Siphonaptera and Phthiraptera mitogenomes. Compared to Phthiraptera with low locomotory capacity, Siphonaptera with high locomotory capacity have higher AT content, slower evolution, and greater influence from natural selection (i.e., micro‐habitat). The mitogenomic evolution of Siphonaptera and Phthiraptera was influenced by locomotory capacity and life history. Phylogenetic analysis based on 13 PCGs showed that Ceratophyllidae, Leptopsyllidae, and Ctenophthalmidae were paraphyletic, and Vermipsyllidae, Hystrichopsyllidae, Pulicidae, and Pygiopsyllidae were monophyletic. This study provides new insights into the phylogenetic relationships and mitogenomic evolution of Siphonaptera.
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spelling doaj-art-40094e30f49e4ca3abc961ee57bcc6a82025-08-20T01:48:44ZengWileyEcology and Evolution2045-77582025-03-01153n/an/a10.1002/ece3.71108Evolution of the Neopsylla hongyangensis Mitogenome: Insights Into the Mitogenomic Evolution of the Orders Siphonaptera and the PhthirapteraXiaoxia Lin0Ju Pu1Wenge Dong2Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Institute of Pathogens and Vectors Dali University Dali Yunnan ChinaYunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Institute of Pathogens and Vectors Dali University Dali Yunnan ChinaYunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Institute of Pathogens and Vectors Dali University Dali Yunnan ChinaABSTRACT The evidence that parasitic animals exhibit elevated mitogenomic evolution rates is inconsistent and limited to Arthropoda. Neopsylla hongyangensis Li, Bai et Chen, 1986 (Siphonaptera: Ctenophthalmidae) feeds on the host's blood and is an important medical insect with plague transmission. In this study, we sequenced the N. hongyangensis mitogenome and explored the mitogenomic evolution of Siphonaptera and Phthiraptera, which both belong to the Insecta on warm‐blooded animals. The mitogenomes of Siphonaptera are closed‐circular double‐stranded DNA molecules and exhibit highly conserved structural features. In contrast, the mitogenomes of most Phthiraptera species exhibit extensive fragmentation and comprise multiple minichromosomes. We performed a comparative analysis of nucleotide composition, Ka/Ks ratios, and codon usage patterns in Siphonaptera and Phthiraptera mitogenomes. Compared to Phthiraptera with low locomotory capacity, Siphonaptera with high locomotory capacity have higher AT content, slower evolution, and greater influence from natural selection (i.e., micro‐habitat). The mitogenomic evolution of Siphonaptera and Phthiraptera was influenced by locomotory capacity and life history. Phylogenetic analysis based on 13 PCGs showed that Ceratophyllidae, Leptopsyllidae, and Ctenophthalmidae were paraphyletic, and Vermipsyllidae, Hystrichopsyllidae, Pulicidae, and Pygiopsyllidae were monophyletic. This study provides new insights into the phylogenetic relationships and mitogenomic evolution of Siphonaptera.https://doi.org/10.1002/ece3.71108evolutionmitogenomeNeopsylla hongyangensisphylogenyplague
spellingShingle Xiaoxia Lin
Ju Pu
Wenge Dong
Evolution of the Neopsylla hongyangensis Mitogenome: Insights Into the Mitogenomic Evolution of the Orders Siphonaptera and the Phthiraptera
Ecology and Evolution
evolution
mitogenome
Neopsylla hongyangensis
phylogeny
plague
title Evolution of the Neopsylla hongyangensis Mitogenome: Insights Into the Mitogenomic Evolution of the Orders Siphonaptera and the Phthiraptera
title_full Evolution of the Neopsylla hongyangensis Mitogenome: Insights Into the Mitogenomic Evolution of the Orders Siphonaptera and the Phthiraptera
title_fullStr Evolution of the Neopsylla hongyangensis Mitogenome: Insights Into the Mitogenomic Evolution of the Orders Siphonaptera and the Phthiraptera
title_full_unstemmed Evolution of the Neopsylla hongyangensis Mitogenome: Insights Into the Mitogenomic Evolution of the Orders Siphonaptera and the Phthiraptera
title_short Evolution of the Neopsylla hongyangensis Mitogenome: Insights Into the Mitogenomic Evolution of the Orders Siphonaptera and the Phthiraptera
title_sort evolution of the neopsylla hongyangensis mitogenome insights into the mitogenomic evolution of the orders siphonaptera and the phthiraptera
topic evolution
mitogenome
Neopsylla hongyangensis
phylogeny
plague
url https://doi.org/10.1002/ece3.71108
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