Host–Pathogen–Vector Continuum in a Changing Landscape: Potential Transmission Pathways for Bartonella in a Small Mammal Community

ABSTRACT Bacterial infections account for a large proportion of zoonoses. Our current understanding of zoonotic spillover, however, is largely based on studies from viral systems. Small mammals such as rodents and their ectoparasites present a unique system for studying several bacterial pathogens a...

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Main Authors: B. R. Ansil, Ashwin Viswanathan, Vivek Ramachandran, H. M. Yeshwanth, Avirup Sanyal, Uma Ramakrishnan
Format: Article
Language:English
Published: Wiley 2025-04-01
Series:Ecology and Evolution
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Online Access:https://doi.org/10.1002/ece3.71085
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author B. R. Ansil
Ashwin Viswanathan
Vivek Ramachandran
H. M. Yeshwanth
Avirup Sanyal
Uma Ramakrishnan
author_facet B. R. Ansil
Ashwin Viswanathan
Vivek Ramachandran
H. M. Yeshwanth
Avirup Sanyal
Uma Ramakrishnan
author_sort B. R. Ansil
collection DOAJ
description ABSTRACT Bacterial infections account for a large proportion of zoonoses. Our current understanding of zoonotic spillover, however, is largely based on studies from viral systems. Small mammals such as rodents and their ectoparasites present a unique system for studying several bacterial pathogens and mapping their spillover pathways. Using Bartonella spp. (a Gram‐negative bacteria) as a model system within a rainforest human‐use landscape, we investigated (1) ecological correlates of Bartonella prevalence in small mammal hosts and (2) evolutionary relationships between Bartonella spp. and various hosts and ectoparasites to gain insight into pathogen movement pathways within ecological communities. We detected Bartonella in five out of eight small mammal species and in 86 (40.56%) out of 212 individuals, but prevalence varied widely among species (0%–75.8%). Seven of the ten ectoparasite species found on these small mammals were positive for Bartonella. Interestingly, while Bartonella genotypes (15) in small mammals were host‐specific, ectoparasites had nonspecific associations, suggesting the possibility for vector‐mediated cross‐species transmission. We also found that Bartonella prevalence in hosts was positively correlated with their aggregated ectoparasite loads, further emphasizing the crucial role that ectoparasites may play in these transmission pathways. Our cophylogenetic analysis and ancestral trait (host) reconstruction revealed incongruence between small mammal and Bartonella phylogenies, indicating historic host shifts and validating the potential for contemporary spillover events. We found that small mammal hosts in this fragmented landscape often move across habitat boundaries, creating a transmission pathway (via shared ectoparasites) to novel hosts, which may include synanthropic species like Rattus rattus. Our results highlight the necessity to disentangle the complex relationship among hosts, ectoparasites, and bacterial pathogens to understand the implications of undetected spillover events.
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spelling doaj-art-3ffcbde9cf4b4fa58648da47ed39a65d2025-08-20T02:35:33ZengWileyEcology and Evolution2045-77582025-04-01154n/an/a10.1002/ece3.71085Host–Pathogen–Vector Continuum in a Changing Landscape: Potential Transmission Pathways for Bartonella in a Small Mammal CommunityB. R. Ansil0Ashwin Viswanathan1Vivek Ramachandran2H. M. Yeshwanth3Avirup Sanyal4Uma Ramakrishnan5National Centre for Biological Sciences Tata Institute of Fundamental Research Bangalore Karnataka IndiaNature Conservation Foundation Mysore Karnataka IndiaNational Centre for Biological Sciences Tata Institute of Fundamental Research Bangalore Karnataka IndiaNational Centre for Biological Sciences Tata Institute of Fundamental Research Bangalore Karnataka IndiaNational Centre for Biological Sciences Tata Institute of Fundamental Research Bangalore Karnataka IndiaNational Centre for Biological Sciences Tata Institute of Fundamental Research Bangalore Karnataka IndiaABSTRACT Bacterial infections account for a large proportion of zoonoses. Our current understanding of zoonotic spillover, however, is largely based on studies from viral systems. Small mammals such as rodents and their ectoparasites present a unique system for studying several bacterial pathogens and mapping their spillover pathways. Using Bartonella spp. (a Gram‐negative bacteria) as a model system within a rainforest human‐use landscape, we investigated (1) ecological correlates of Bartonella prevalence in small mammal hosts and (2) evolutionary relationships between Bartonella spp. and various hosts and ectoparasites to gain insight into pathogen movement pathways within ecological communities. We detected Bartonella in five out of eight small mammal species and in 86 (40.56%) out of 212 individuals, but prevalence varied widely among species (0%–75.8%). Seven of the ten ectoparasite species found on these small mammals were positive for Bartonella. Interestingly, while Bartonella genotypes (15) in small mammals were host‐specific, ectoparasites had nonspecific associations, suggesting the possibility for vector‐mediated cross‐species transmission. We also found that Bartonella prevalence in hosts was positively correlated with their aggregated ectoparasite loads, further emphasizing the crucial role that ectoparasites may play in these transmission pathways. Our cophylogenetic analysis and ancestral trait (host) reconstruction revealed incongruence between small mammal and Bartonella phylogenies, indicating historic host shifts and validating the potential for contemporary spillover events. We found that small mammal hosts in this fragmented landscape often move across habitat boundaries, creating a transmission pathway (via shared ectoparasites) to novel hosts, which may include synanthropic species like Rattus rattus. Our results highlight the necessity to disentangle the complex relationship among hosts, ectoparasites, and bacterial pathogens to understand the implications of undetected spillover events.https://doi.org/10.1002/ece3.71085bacterial zoonosiscoevolutionectoparasiteshost shiftsvector‐borne pathogens
spellingShingle B. R. Ansil
Ashwin Viswanathan
Vivek Ramachandran
H. M. Yeshwanth
Avirup Sanyal
Uma Ramakrishnan
Host–Pathogen–Vector Continuum in a Changing Landscape: Potential Transmission Pathways for Bartonella in a Small Mammal Community
Ecology and Evolution
bacterial zoonosis
coevolution
ectoparasites
host shifts
vector‐borne pathogens
title Host–Pathogen–Vector Continuum in a Changing Landscape: Potential Transmission Pathways for Bartonella in a Small Mammal Community
title_full Host–Pathogen–Vector Continuum in a Changing Landscape: Potential Transmission Pathways for Bartonella in a Small Mammal Community
title_fullStr Host–Pathogen–Vector Continuum in a Changing Landscape: Potential Transmission Pathways for Bartonella in a Small Mammal Community
title_full_unstemmed Host–Pathogen–Vector Continuum in a Changing Landscape: Potential Transmission Pathways for Bartonella in a Small Mammal Community
title_short Host–Pathogen–Vector Continuum in a Changing Landscape: Potential Transmission Pathways for Bartonella in a Small Mammal Community
title_sort host pathogen vector continuum in a changing landscape potential transmission pathways for bartonella in a small mammal community
topic bacterial zoonosis
coevolution
ectoparasites
host shifts
vector‐borne pathogens
url https://doi.org/10.1002/ece3.71085
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