Effects of calcium lactate on in vitro fertilization and embryonic development in cattle
Objective Growing demand for embryo transfer is steadily expanding and further studies on in vitro fertilization of cattle. To assess the effect of calcium lactate by replacing Tyrode’s albumin lactate pyruvate (TALP) medium composition during fertilization and embryonic development. Methods Sodium...
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Asian-Australasian Association of Animal Production Societies
2025-06-01
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| Series: | Animal Bioscience |
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| Online Access: | http://www.animbiosci.org/upload/pdf/ab-24-0636.pdf |
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| author | Bo-myeong Kim Song-Hee Lee Geun Heo Ji-Dam Kim Gyu-Hyun Lee Jae-Min Sim Kwang Taek Lim Xiang-Shun Cui |
| author_facet | Bo-myeong Kim Song-Hee Lee Geun Heo Ji-Dam Kim Gyu-Hyun Lee Jae-Min Sim Kwang Taek Lim Xiang-Shun Cui |
| author_sort | Bo-myeong Kim |
| collection | DOAJ |
| description | Objective Growing demand for embryo transfer is steadily expanding and further studies on in vitro fertilization of cattle. To assess the effect of calcium lactate by replacing Tyrode’s albumin lactate pyruvate (TALP) medium composition during fertilization and embryonic development. Methods Sodium lactate and CaCl2 were replaced with 2.0, 3.0, 4.5 mM calcium lactate for TALP medium during fertilization in Experiment 1. In Experiment 2, the concentrations of sodium lactate and CaCl2 were re-modified as control, in comparison with the same concentration of calcium lactate at 4.5 mM. Zygotes were moved to sequential media to match early-and late-stage environments. Embryonic development was examined on day 8 after insemination. Results A 4.5 mM calcium lactate enhanced the rate of fertilization and blastocyst formation (p<0.0001, p<0.01, respectively). It represented differences in the reactive oxygen species (ROS) (p<0.01) and glutathione (GSH) levels (p<0.05) and increased blastocyst diameter and total cell number (p<0.05). In Experiment 2, fertilization (p<0.05) and blastocyst formation rates (p<0.01) were increased in 4.5mM calcium lactate under same concentration effect of sodium lactate and CaCl2. Additionally, it reduced the ROS (p<0.01) and increased the GSH levels (p<0.05), leading increase embryo quality. Conclusion The replacement of calcium lactate in TALP medium enhances fertilization and embryonic development while also improving oxidative stress. Specifically, it has been determined that a concentration of 4.5 mM calcium lactate is the most effective, irrespective of the varying concentrations of sodium lactate and CaCl2. This study presents a novel formulation of a modified TALP medium intended for implantation withing the bovine embryo industry. The current implications of the study are discussed in relation to previously stated objectives and hypotheses. |
| format | Article |
| id | doaj-art-3ff7437b711d4bc8af654344288602aa |
| institution | OA Journals |
| issn | 2765-0189 2765-0235 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Animal Bioscience |
| spelling | doaj-art-3ff7437b711d4bc8af654344288602aa2025-08-20T02:16:10ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352025-06-013861150115910.5713/ab.24.063625389Effects of calcium lactate on in vitro fertilization and embryonic development in cattleBo-myeong Kim0Song-Hee Lee1Geun Heo2Ji-Dam Kim3Gyu-Hyun Lee4Jae-Min Sim5Kwang Taek Lim6Xiang-Shun Cui7 Department of Animal Science, Chungbuk National University, Cheongju, Korea Department of Animal Science, Chungbuk National University, Cheongju, Korea Department of Animal Science, Chungbuk National University, Cheongju, Korea Department of Animal Science, Chungbuk National University, Cheongju, Korea Department of Animal Science, Chungbuk National University, Cheongju, Korea Department of Animal Science, Chungbuk National University, Cheongju, Korea Genetics, Anseong, Korea Department of Animal Science, Chungbuk National University, Cheongju, KoreaObjective Growing demand for embryo transfer is steadily expanding and further studies on in vitro fertilization of cattle. To assess the effect of calcium lactate by replacing Tyrode’s albumin lactate pyruvate (TALP) medium composition during fertilization and embryonic development. Methods Sodium lactate and CaCl2 were replaced with 2.0, 3.0, 4.5 mM calcium lactate for TALP medium during fertilization in Experiment 1. In Experiment 2, the concentrations of sodium lactate and CaCl2 were re-modified as control, in comparison with the same concentration of calcium lactate at 4.5 mM. Zygotes were moved to sequential media to match early-and late-stage environments. Embryonic development was examined on day 8 after insemination. Results A 4.5 mM calcium lactate enhanced the rate of fertilization and blastocyst formation (p<0.0001, p<0.01, respectively). It represented differences in the reactive oxygen species (ROS) (p<0.01) and glutathione (GSH) levels (p<0.05) and increased blastocyst diameter and total cell number (p<0.05). In Experiment 2, fertilization (p<0.05) and blastocyst formation rates (p<0.01) were increased in 4.5mM calcium lactate under same concentration effect of sodium lactate and CaCl2. Additionally, it reduced the ROS (p<0.01) and increased the GSH levels (p<0.05), leading increase embryo quality. Conclusion The replacement of calcium lactate in TALP medium enhances fertilization and embryonic development while also improving oxidative stress. Specifically, it has been determined that a concentration of 4.5 mM calcium lactate is the most effective, irrespective of the varying concentrations of sodium lactate and CaCl2. This study presents a novel formulation of a modified TALP medium intended for implantation withing the bovine embryo industry. The current implications of the study are discussed in relation to previously stated objectives and hypotheses.http://www.animbiosci.org/upload/pdf/ab-24-0636.pdfcalcium lactatecattleembryonic developmentin vitro fertilizationoxidative stresstalp medium |
| spellingShingle | Bo-myeong Kim Song-Hee Lee Geun Heo Ji-Dam Kim Gyu-Hyun Lee Jae-Min Sim Kwang Taek Lim Xiang-Shun Cui Effects of calcium lactate on in vitro fertilization and embryonic development in cattle Animal Bioscience calcium lactate cattle embryonic development in vitro fertilization oxidative stress talp medium |
| title | Effects of calcium lactate on in vitro fertilization and embryonic development in cattle |
| title_full | Effects of calcium lactate on in vitro fertilization and embryonic development in cattle |
| title_fullStr | Effects of calcium lactate on in vitro fertilization and embryonic development in cattle |
| title_full_unstemmed | Effects of calcium lactate on in vitro fertilization and embryonic development in cattle |
| title_short | Effects of calcium lactate on in vitro fertilization and embryonic development in cattle |
| title_sort | effects of calcium lactate on in vitro fertilization and embryonic development in cattle |
| topic | calcium lactate cattle embryonic development in vitro fertilization oxidative stress talp medium |
| url | http://www.animbiosci.org/upload/pdf/ab-24-0636.pdf |
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