Propionibacterium freudenreichii co-inoculation enhances Bifidobacterium longum subsp. longum BB536 growth and metabolite content in soy whey

Growth of human resident bifidobacteria in nutrient-scarce food matrices such as unsupplemented soy whey remains a challenge. The aim of this study was to explore the potential synergy and metabolite changes when simultaneously inoculating probiotic Bifidobacterium longum subsp. longum with Propioni...

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Main Authors: Ricco Tindjau, Bing-Qi Liang, Shruti Srinivasan, Jian-Yong Chua, Shao-Quan Liu
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:The Microbe
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Online Access:http://www.sciencedirect.com/science/article/pii/S2950194625002146
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author Ricco Tindjau
Bing-Qi Liang
Shruti Srinivasan
Jian-Yong Chua
Shao-Quan Liu
author_facet Ricco Tindjau
Bing-Qi Liang
Shruti Srinivasan
Jian-Yong Chua
Shao-Quan Liu
author_sort Ricco Tindjau
collection DOAJ
description Growth of human resident bifidobacteria in nutrient-scarce food matrices such as unsupplemented soy whey remains a challenge. The aim of this study was to explore the potential synergy and metabolite changes when simultaneously inoculating probiotic Bifidobacterium longum subsp. longum with Propionibacterium freudenreichii in unsupplemented soy whey. Parameters such as growth, short-chain fatty acids (SCFA), and vitamin B12 were assessed to uncover underlying effects in monoculture and coculture systems. P. freudenreichii monoculture showed similar growth patterns when grown in mono- or coculture. Conversely, B. longum monoculture was unable to grow in unsupplemented soy whey, entering the death phase by day 1 of fermentation. However, B. longum growth was significantly improved (∼2 log CFU/mL) when cultured simultaneously with P. freudenreichii. Furthermore, SCFA contents were also enhanced, with B12 content peaking 2 days earlier in coculture compared to monoculture. Hence, co-culturing B. longum with P. freudenreichii could provide a supplement-free solution to valorising soy whey into a potential beverage, by improving probiotic bifidobacterial count, SCFA and vitamin B12 contents.
format Article
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institution Kabale University
issn 2950-1946
language English
publishDate 2025-09-01
publisher Elsevier
record_format Article
series The Microbe
spelling doaj-art-3fe5ca5dd6604f5d8530a7d7f683fd882025-08-20T03:50:21ZengElsevierThe Microbe2950-19462025-09-01810044610.1016/j.microb.2025.100446Propionibacterium freudenreichii co-inoculation enhances Bifidobacterium longum subsp. longum BB536 growth and metabolite content in soy wheyRicco Tindjau0Bing-Qi Liang1Shruti Srinivasan2Jian-Yong Chua3Shao-Quan Liu4Department of Food Science and Technology, National University of Singapore, 2 Science Drive 2, Singapore 117542, SingaporeDepartment of Food Science and Technology, National University of Singapore, 2 Science Drive 2, Singapore 117542, SingaporeDepartment of Food Science and Technology, National University of Singapore, 2 Science Drive 2, Singapore 117542, SingaporeDepartment of Food Science and Technology, National University of Singapore, 2 Science Drive 2, Singapore 117542, SingaporeDepartment of Food Science and Technology, National University of Singapore, 2 Science Drive 2, Singapore 117542, Singapore; National University of Singapore (Suzhou) Research Institute, 377 Lin Quan Street, Suzhou Industrial Park, Jiangsu 215213, China; Corresponding author at: Department of Food Science and Technology, National University of Singapore, 2 Science Drive 2, Singapore 117542, Singapore.Growth of human resident bifidobacteria in nutrient-scarce food matrices such as unsupplemented soy whey remains a challenge. The aim of this study was to explore the potential synergy and metabolite changes when simultaneously inoculating probiotic Bifidobacterium longum subsp. longum with Propionibacterium freudenreichii in unsupplemented soy whey. Parameters such as growth, short-chain fatty acids (SCFA), and vitamin B12 were assessed to uncover underlying effects in monoculture and coculture systems. P. freudenreichii monoculture showed similar growth patterns when grown in mono- or coculture. Conversely, B. longum monoculture was unable to grow in unsupplemented soy whey, entering the death phase by day 1 of fermentation. However, B. longum growth was significantly improved (∼2 log CFU/mL) when cultured simultaneously with P. freudenreichii. Furthermore, SCFA contents were also enhanced, with B12 content peaking 2 days earlier in coculture compared to monoculture. Hence, co-culturing B. longum with P. freudenreichii could provide a supplement-free solution to valorising soy whey into a potential beverage, by improving probiotic bifidobacterial count, SCFA and vitamin B12 contents.http://www.sciencedirect.com/science/article/pii/S2950194625002146Human resident bifidobacteriaCocultureValorizationShort-chain fatty acidsVitamin B12
spellingShingle Ricco Tindjau
Bing-Qi Liang
Shruti Srinivasan
Jian-Yong Chua
Shao-Quan Liu
Propionibacterium freudenreichii co-inoculation enhances Bifidobacterium longum subsp. longum BB536 growth and metabolite content in soy whey
The Microbe
Human resident bifidobacteria
Coculture
Valorization
Short-chain fatty acids
Vitamin B12
title Propionibacterium freudenreichii co-inoculation enhances Bifidobacterium longum subsp. longum BB536 growth and metabolite content in soy whey
title_full Propionibacterium freudenreichii co-inoculation enhances Bifidobacterium longum subsp. longum BB536 growth and metabolite content in soy whey
title_fullStr Propionibacterium freudenreichii co-inoculation enhances Bifidobacterium longum subsp. longum BB536 growth and metabolite content in soy whey
title_full_unstemmed Propionibacterium freudenreichii co-inoculation enhances Bifidobacterium longum subsp. longum BB536 growth and metabolite content in soy whey
title_short Propionibacterium freudenreichii co-inoculation enhances Bifidobacterium longum subsp. longum BB536 growth and metabolite content in soy whey
title_sort propionibacterium freudenreichii co inoculation enhances bifidobacterium longum subsp longum bb536 growth and metabolite content in soy whey
topic Human resident bifidobacteria
Coculture
Valorization
Short-chain fatty acids
Vitamin B12
url http://www.sciencedirect.com/science/article/pii/S2950194625002146
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