Propionibacterium freudenreichii co-inoculation enhances Bifidobacterium longum subsp. longum BB536 growth and metabolite content in soy whey
Growth of human resident bifidobacteria in nutrient-scarce food matrices such as unsupplemented soy whey remains a challenge. The aim of this study was to explore the potential synergy and metabolite changes when simultaneously inoculating probiotic Bifidobacterium longum subsp. longum with Propioni...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-09-01
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| Series: | The Microbe |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2950194625002146 |
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| Summary: | Growth of human resident bifidobacteria in nutrient-scarce food matrices such as unsupplemented soy whey remains a challenge. The aim of this study was to explore the potential synergy and metabolite changes when simultaneously inoculating probiotic Bifidobacterium longum subsp. longum with Propionibacterium freudenreichii in unsupplemented soy whey. Parameters such as growth, short-chain fatty acids (SCFA), and vitamin B12 were assessed to uncover underlying effects in monoculture and coculture systems. P. freudenreichii monoculture showed similar growth patterns when grown in mono- or coculture. Conversely, B. longum monoculture was unable to grow in unsupplemented soy whey, entering the death phase by day 1 of fermentation. However, B. longum growth was significantly improved (∼2 log CFU/mL) when cultured simultaneously with P. freudenreichii. Furthermore, SCFA contents were also enhanced, with B12 content peaking 2 days earlier in coculture compared to monoculture. Hence, co-culturing B. longum with P. freudenreichii could provide a supplement-free solution to valorising soy whey into a potential beverage, by improving probiotic bifidobacterial count, SCFA and vitamin B12 contents. |
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| ISSN: | 2950-1946 |