Identification of PA28β as a potential novel biomarker in human esophageal squamous cell carcinoma
Esophageal squamous cell carcinoma (ESCC) is one of the most common and serious malignancies in China. However, the exact mechanisms of tumor formation and progression are unclear. As late diagnosis and poor therapeutic efficacy result in lower survival rates, identifying biomarkers for early detect...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2017-10-01
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| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317719780 |
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| author | Jin-Yan Chen Li Xu Wei-Min Fang Jun-Yong Han Kun Wang Kun-Shou Zhu |
| author_facet | Jin-Yan Chen Li Xu Wei-Min Fang Jun-Yong Han Kun Wang Kun-Shou Zhu |
| author_sort | Jin-Yan Chen |
| collection | DOAJ |
| description | Esophageal squamous cell carcinoma (ESCC) is one of the most common and serious malignancies in China. However, the exact mechanisms of tumor formation and progression are unclear. As late diagnosis and poor therapeutic efficacy result in lower survival rates, identifying biomarkers for early detection, prognostic evaluation, and recurrence monitoring of ESCC is necessary. Here we analyzed 10 protein expression profiles of ESCC core tissues and paired normal esophageal epithelial tissues using two-dimensional gel electrophoresis. We excised 29 protein spots with two-fold or greater differential expression between cancer and normal tissues and identified them using matrix-assisted laser desorption/ionization–time-of-flight/time-of-flight mass spectrometry. The role of PA28β in ESCC cell was confirmed using cell growth, colony formation and soft agar in TE-1 cells pre- and post- PA28β transfection. Compared to their expression in the adjacent normal epithelia, 12 proteins, including transgelin (TAGLN), were upregulated in ESCC tissues; 17 proteins, including proteasome activator 28-beta subunit (PA28β), were downregulated (p < 0.05). Western blotting and immunohistochemistry confirmed that PA28β was significantly underexpressed in ESCC tissues. The functional assays demonstrate that PA28β inhibited cell growth, proliferation and malignancy of TE-1 cells. Among the differentially expressed proteins, PA28β is a potential tumor inhibitor. |
| format | Article |
| id | doaj-art-3fe25435d54d4797ad12035ae2abdd3d |
| institution | DOAJ |
| issn | 1423-0380 |
| language | English |
| publishDate | 2017-10-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj-art-3fe25435d54d4797ad12035ae2abdd3d2025-08-20T02:52:38ZengSAGE PublishingTumor Biology1423-03802017-10-013910.1177/1010428317719780Identification of PA28β as a potential novel biomarker in human esophageal squamous cell carcinomaJin-Yan Chen0Li Xu1Wei-Min Fang2Jun-Yong Han3Kun Wang4Kun-Shou Zhu5Fujian Provincial Key Laboratory of Medical Analysis, Fuzhou, ChinaDepartment of Physiology, Basic Medical College of Putian University, Putian, ChinaFujian Provincial Cancer Hospital, Fuzhou, ChinaFujian Provincial Key Laboratory of Medical Analysis, Fuzhou, ChinaFujian Provincial Key Laboratory of Medical Analysis, Fuzhou, ChinaFujian Provincial Cancer Hospital, Fuzhou, ChinaEsophageal squamous cell carcinoma (ESCC) is one of the most common and serious malignancies in China. However, the exact mechanisms of tumor formation and progression are unclear. As late diagnosis and poor therapeutic efficacy result in lower survival rates, identifying biomarkers for early detection, prognostic evaluation, and recurrence monitoring of ESCC is necessary. Here we analyzed 10 protein expression profiles of ESCC core tissues and paired normal esophageal epithelial tissues using two-dimensional gel electrophoresis. We excised 29 protein spots with two-fold or greater differential expression between cancer and normal tissues and identified them using matrix-assisted laser desorption/ionization–time-of-flight/time-of-flight mass spectrometry. The role of PA28β in ESCC cell was confirmed using cell growth, colony formation and soft agar in TE-1 cells pre- and post- PA28β transfection. Compared to their expression in the adjacent normal epithelia, 12 proteins, including transgelin (TAGLN), were upregulated in ESCC tissues; 17 proteins, including proteasome activator 28-beta subunit (PA28β), were downregulated (p < 0.05). Western blotting and immunohistochemistry confirmed that PA28β was significantly underexpressed in ESCC tissues. The functional assays demonstrate that PA28β inhibited cell growth, proliferation and malignancy of TE-1 cells. Among the differentially expressed proteins, PA28β is a potential tumor inhibitor.https://doi.org/10.1177/1010428317719780 |
| spellingShingle | Jin-Yan Chen Li Xu Wei-Min Fang Jun-Yong Han Kun Wang Kun-Shou Zhu Identification of PA28β as a potential novel biomarker in human esophageal squamous cell carcinoma Tumor Biology |
| title | Identification of PA28β as a potential novel biomarker in human esophageal squamous cell carcinoma |
| title_full | Identification of PA28β as a potential novel biomarker in human esophageal squamous cell carcinoma |
| title_fullStr | Identification of PA28β as a potential novel biomarker in human esophageal squamous cell carcinoma |
| title_full_unstemmed | Identification of PA28β as a potential novel biomarker in human esophageal squamous cell carcinoma |
| title_short | Identification of PA28β as a potential novel biomarker in human esophageal squamous cell carcinoma |
| title_sort | identification of pa28β as a potential novel biomarker in human esophageal squamous cell carcinoma |
| url | https://doi.org/10.1177/1010428317719780 |
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