Establishment and characterization of multilayered fibroblast cell sheets from the canine oral mucosa, skin, and tail skin
Background: Cell sheet therapy has been developed as an effective regenerative medicine to improve wound repair treatment and prevent postoperative complications. Fibroblasts are widely used to create cell sheet engineering because of their essential role in tissue regeneration and the acceleration...
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| Format: | Article |
| Language: | English |
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Tripoli University
2024-12-01
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| Series: | Open Veterinary Journal |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=222546 |
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| author | Melpa Susanti Purba Dito Anggoro Naohiro Yamamoto Sota Yoshimine Junichi Murakami Toshiki Tanaka Kimikazu Hamano Harumichi Itoh Kazuhito Itamoto Yuki Nemoto Munekazu Nakaichi Hiroshi Sunahara Kenji Tani |
| author_facet | Melpa Susanti Purba Dito Anggoro Naohiro Yamamoto Sota Yoshimine Junichi Murakami Toshiki Tanaka Kimikazu Hamano Harumichi Itoh Kazuhito Itamoto Yuki Nemoto Munekazu Nakaichi Hiroshi Sunahara Kenji Tani |
| author_sort | Melpa Susanti Purba |
| collection | DOAJ |
| description | Background:
Cell sheet therapy has been developed as an effective regenerative medicine to improve wound repair treatment and prevent postoperative complications. Fibroblasts are widely used to create cell sheet engineering because of their essential role in tissue regeneration and the acceleration of the wound healing process.
Aim:
The study aimed to establish the multilayered fibroblast sheets from canine fibroblast.
Methods:
The study used fibroblasts from three origin canine tissues, including oral mucosa, skin, and tail skin. A canine fibroblast sheet was produced using the large-numbers cell seeding method with Rho kinase inhibitor. The assessment included viability cells, histological evaluation of the sheet, and secretion of vascular endothelial growth factor (VEGF) and monocyte chemoattractant protein-1 (MCP-1) of the fibroblast sheet.
Results:
The three fibroblast groups successfully established a multilayered fibroblast sheet. Hematoxylin and eosin staining examination showed the thickest sheet in the oral mucosa fibroblast sheet. Immunohistochemical examination revealed that the multilayered cell sheet comprised fibroblasts expressed by vimentin. All multilayered fibroblast sheet groups secreted the VEGF and MCP-1. No differences were observed in the viability cells of each origin cell.
Conclusion:
The multilayered fibroblast sheets were successfully established from canine fibroblast. This finding provides the primary data to develop regenerative medicine in the veterinary field. [Open Vet J 2024; 14(12.000): 3428-3439] |
| format | Article |
| id | doaj-art-3fdaebbaf1e34d8c99c4b28ac83628bb |
| institution | OA Journals |
| issn | 2226-4485 2218-6050 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Tripoli University |
| record_format | Article |
| series | Open Veterinary Journal |
| spelling | doaj-art-3fdaebbaf1e34d8c99c4b28ac83628bb2025-08-20T02:16:02ZengTripoli UniversityOpen Veterinary Journal2226-44852218-60502024-12-0114123428343910.5455/OVJ.2024.v14.i12.27222546Establishment and characterization of multilayered fibroblast cell sheets from the canine oral mucosa, skin, and tail skinMelpa Susanti Purba0Dito Anggoro1Naohiro Yamamoto2Sota Yoshimine3Junichi Murakami4Toshiki Tanaka5Kimikazu Hamano6Harumichi Itoh7Kazuhito Itamoto8Yuki Nemoto9Munekazu Nakaichi10Hiroshi Sunahara11Kenji Tani12Joint Graduate School of Veterinary Medicine, Yamaguchi University, Japan, Laboratory of Veterinary Surgery, Joint Faculty of Veterinary Medicine, Yamaguchi University, Japan Joint Graduate School of Veterinary Medicine, Yamaguchi University, Japan, Laboratory of Veterinary Surgery, Joint Faculty of Veterinary Medicine, Yamaguchi University, Japan, Department of Surgery and Radiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Indonesia Department of Surgery and Clinical Science, Graduate School of Medicine, Yamaguchi University, Ube, Yamaguchi, Japan Department of Surgery and Clinical Science, Graduate School of Medicine, Yamaguchi University, Ube, Yamaguchi, Japan Department of Surgery and Clinical Science, Graduate School of Medicine, Yamaguchi University, Ube, Yamaguchi, Japan Department of Surgery and Clinical Science, Graduate School of Medicine, Yamaguchi University, Ube, Yamaguchi, Japan Department of Surgery and Clinical Science, Graduate School of Medicine, Yamaguchi University, Ube, Yamaguchi, Japan Laboratory of Small Animal Clinical Science, Joint Faculty of Veterinary Medicine, Yamaguchi University, Japan Laboratory of Small Animal Clinical Science, Joint Faculty of Veterinary Medicine, Yamaguchi University, Japan Laboratory of Veterinary Radiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Japan Laboratory of Veterinary Radiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Japan Laboratory of Veterinary Surgery, Joint Faculty of Veterinary Medicine, Yamaguchi University, Japan Laboratory of Veterinary Surgery, Joint Faculty of Veterinary Medicine, Yamaguchi University, JapanBackground: Cell sheet therapy has been developed as an effective regenerative medicine to improve wound repair treatment and prevent postoperative complications. Fibroblasts are widely used to create cell sheet engineering because of their essential role in tissue regeneration and the acceleration of the wound healing process. Aim: The study aimed to establish the multilayered fibroblast sheets from canine fibroblast. Methods: The study used fibroblasts from three origin canine tissues, including oral mucosa, skin, and tail skin. A canine fibroblast sheet was produced using the large-numbers cell seeding method with Rho kinase inhibitor. The assessment included viability cells, histological evaluation of the sheet, and secretion of vascular endothelial growth factor (VEGF) and monocyte chemoattractant protein-1 (MCP-1) of the fibroblast sheet. Results: The three fibroblast groups successfully established a multilayered fibroblast sheet. Hematoxylin and eosin staining examination showed the thickest sheet in the oral mucosa fibroblast sheet. Immunohistochemical examination revealed that the multilayered cell sheet comprised fibroblasts expressed by vimentin. All multilayered fibroblast sheet groups secreted the VEGF and MCP-1. No differences were observed in the viability cells of each origin cell. Conclusion: The multilayered fibroblast sheets were successfully established from canine fibroblast. This finding provides the primary data to develop regenerative medicine in the veterinary field. [Open Vet J 2024; 14(12.000): 3428-3439]http://www.ejmanager.com/fulltextpdf.php?mno=222546angiogenesiscanineregenerative medicinerho kinase inhibitor |
| spellingShingle | Melpa Susanti Purba Dito Anggoro Naohiro Yamamoto Sota Yoshimine Junichi Murakami Toshiki Tanaka Kimikazu Hamano Harumichi Itoh Kazuhito Itamoto Yuki Nemoto Munekazu Nakaichi Hiroshi Sunahara Kenji Tani Establishment and characterization of multilayered fibroblast cell sheets from the canine oral mucosa, skin, and tail skin Open Veterinary Journal angiogenesis canine regenerative medicine rho kinase inhibitor |
| title | Establishment and characterization of multilayered fibroblast cell sheets from the canine oral mucosa, skin, and tail skin |
| title_full | Establishment and characterization of multilayered fibroblast cell sheets from the canine oral mucosa, skin, and tail skin |
| title_fullStr | Establishment and characterization of multilayered fibroblast cell sheets from the canine oral mucosa, skin, and tail skin |
| title_full_unstemmed | Establishment and characterization of multilayered fibroblast cell sheets from the canine oral mucosa, skin, and tail skin |
| title_short | Establishment and characterization of multilayered fibroblast cell sheets from the canine oral mucosa, skin, and tail skin |
| title_sort | establishment and characterization of multilayered fibroblast cell sheets from the canine oral mucosa skin and tail skin |
| topic | angiogenesis canine regenerative medicine rho kinase inhibitor |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=222546 |
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