Phylogenetic and expression analysis of terpenoid synthase gene family in Eustoma grandiflorum
Terpenoids are one of the main components of floral fragrance, and terpenoid synthase (TPS) plays a key role in the synthesis of terpenoids. In this study, 26 full-length TPS genes were identified using bioinformatics methods, and their molecular evolution, gene structure, and expression patterns we...
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Zhejiang University Press
2024-10-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2023.06.121 |
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| author | LI Xinyuan LIANG Yuwei FANG Huiyi SUN Fuhui ZHANG Liangsheng |
| author_facet | LI Xinyuan LIANG Yuwei FANG Huiyi SUN Fuhui ZHANG Liangsheng |
| author_sort | LI Xinyuan |
| collection | DOAJ |
| description | Terpenoids are one of the main components of floral fragrance, and terpenoid synthase (TPS) plays a key role in the synthesis of terpenoids. In this study, 26 full-length TPS genes were identified using bioinformatics methods, and their molecular evolution, gene structure, and expression patterns were analyzed. The results revealed that the TPS genes of Eustoma grandiflorum could be classified into five subfamilies (TPS-a, TPS-b, TPS-g, TPS-e, and TPS-f), which were further divided into 10 smaller clades. Among these clades, only the TPS-a.1 clade exhibited amplification, while most of the other clades presented gene loss. Collinearity analysis of genes demonstrated that five collinear gene pairs were formed through two recent whole genome duplication events in E. grandiflorum, and most of the TPS genes were lost, further supporting the conclusion that most TPS genes in E. grandiflorum have been lost. Transcriptome expression analysis showed that only EgTPS-g1, EgTPS-g2, and EgTPS-g3 were expressed in the early stages of petal development, and TPS-a and TPS-b subfamily members were not expressed in the petals of E. grandiflorum, likely contributing to the lack of floral fragrance. Future research should focus on TPS-a and TPS-b subfamily members and restore the expression of these subfamily genes, which are expected to give the floral fragrance to E. grandiflorum and thus improve its ornamental value. |
| format | Article |
| id | doaj-art-3fd8b8e705ae468daa0d512b17a4fd30 |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Zhejiang University Press |
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| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-3fd8b8e705ae468daa0d512b17a4fd302025-08-20T02:47:26ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552024-10-015073774610.3785/j.issn.1008-9209.2023.06.12110089209Phylogenetic and expression analysis of terpenoid synthase gene family in Eustoma grandiflorumLI XinyuanLIANG YuweiFANG HuiyiSUN FuhuiZHANG LiangshengTerpenoids are one of the main components of floral fragrance, and terpenoid synthase (TPS) plays a key role in the synthesis of terpenoids. In this study, 26 full-length TPS genes were identified using bioinformatics methods, and their molecular evolution, gene structure, and expression patterns were analyzed. The results revealed that the TPS genes of Eustoma grandiflorum could be classified into five subfamilies (TPS-a, TPS-b, TPS-g, TPS-e, and TPS-f), which were further divided into 10 smaller clades. Among these clades, only the TPS-a.1 clade exhibited amplification, while most of the other clades presented gene loss. Collinearity analysis of genes demonstrated that five collinear gene pairs were formed through two recent whole genome duplication events in E. grandiflorum, and most of the TPS genes were lost, further supporting the conclusion that most TPS genes in E. grandiflorum have been lost. Transcriptome expression analysis showed that only EgTPS-g1, EgTPS-g2, and EgTPS-g3 were expressed in the early stages of petal development, and TPS-a and TPS-b subfamily members were not expressed in the petals of E. grandiflorum, likely contributing to the lack of floral fragrance. Future research should focus on TPS-a and TPS-b subfamily members and restore the expression of these subfamily genes, which are expected to give the floral fragrance to E. grandiflorum and thus improve its ornamental value.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2023.06.121<italic>Eustoma grandiflorum</italic> Gentianalesterpenoid synthase (TPS) gene familyfloral fragranceexpression analysis |
| spellingShingle | LI Xinyuan LIANG Yuwei FANG Huiyi SUN Fuhui ZHANG Liangsheng Phylogenetic and expression analysis of terpenoid synthase gene family in Eustoma grandiflorum 浙江大学学报. 农业与生命科学版 <italic>Eustoma grandiflorum</italic> Gentianales terpenoid synthase (TPS) gene family floral fragrance expression analysis |
| title | Phylogenetic and expression analysis of terpenoid synthase gene family in Eustoma grandiflorum |
| title_full | Phylogenetic and expression analysis of terpenoid synthase gene family in Eustoma grandiflorum |
| title_fullStr | Phylogenetic and expression analysis of terpenoid synthase gene family in Eustoma grandiflorum |
| title_full_unstemmed | Phylogenetic and expression analysis of terpenoid synthase gene family in Eustoma grandiflorum |
| title_short | Phylogenetic and expression analysis of terpenoid synthase gene family in Eustoma grandiflorum |
| title_sort | phylogenetic and expression analysis of terpenoid synthase gene family in eustoma grandiflorum |
| topic | <italic>Eustoma grandiflorum</italic> Gentianales terpenoid synthase (TPS) gene family floral fragrance expression analysis |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2023.06.121 |
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