Commensal and virulent Escherichia coli strains of vaginal origin are reservoirs of resistance cassettes in class 1 integrons
Introduction: Antimicrobial resistance in Escherichia coli, one of the causal agents of aerobic vaginitis, leads to the persistence of the infection. The investigation of integrons acquires relevance, since they are elements that are responsible for the acquisition of resistance to antibiotics. The...
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The Journal of Infection in Developing Countries
2020-01-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/11940 |
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| author | Manalli Itzahaya Blancarte–Lagunas Graciela Castro-Escarpulli Armando Navarro-Ocaña Gabriela Ibáñez-Cervantes Laura Margarita Marquez-Valdelamar José Misael Hernández-Carrillo Juana Salazar-Salinas Omar Fernando Mendoza-Vásquez Gabriel Damazo-Hernández Oscar Sosa-Hernández Gregorio León-García Monica Alethia Cureño-Díaz Juan Manuel Bello-López |
| author_facet | Manalli Itzahaya Blancarte–Lagunas Graciela Castro-Escarpulli Armando Navarro-Ocaña Gabriela Ibáñez-Cervantes Laura Margarita Marquez-Valdelamar José Misael Hernández-Carrillo Juana Salazar-Salinas Omar Fernando Mendoza-Vásquez Gabriel Damazo-Hernández Oscar Sosa-Hernández Gregorio León-García Monica Alethia Cureño-Díaz Juan Manuel Bello-López |
| author_sort | Manalli Itzahaya Blancarte–Lagunas |
| collection | DOAJ |
| description |
Introduction: Antimicrobial resistance in Escherichia coli, one of the causal agents of aerobic vaginitis, leads to the persistence of the infection. The investigation of integrons acquires relevance, since they are elements that are responsible for the acquisition of resistance to antibiotics. The aim of this work was to describe the structural diversity of class 1 integrons in virulent and commensal strains of E. coli isolated from patients with vaginal infection.
Methodology: Ninety-two strains of E. coli were isolated from patients with aerobic vaginitis. Resistance profile against 19 antibiotics and class 1 integrons were detected by PCR. The identity and arrangement of cassettes was determined by sequencing. ERIC-PCR assays were carried out in strains with identical arrays. Finally, genotyping by Clermont algorithm and serotyping were performed. Seventeen strains showed integrons located in plasmids.
Results: Ten different gene cassette arrays were identified in 30 strains of E. coli. Cassettes corresponding to genes coding for adenylyltransferases (aadA), dihydrofolate reductases (dfrA), and oxacillinases (blaOXA) were detected. Array dfrA17-aadA5 was predominantly prevalent over the other arrays identified. Phylogenetic group A was the most predominant, followed by group B2 and D.
Conclusions: This study demonstrates the presence of E. coli of vaginal origin carrying class 1 integrons, which are main genetic elements of capture of resistance genes, with the possibility of capturing new resistance cassettes. These evidences should serve for the modification of protocols in the diagnosis and treatment of aerobic vaginitis, and the development of policies for the rational use of antimicrobials.
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| format | Article |
| id | doaj-art-3fd7681c2ca0472d8e87458ca7322990 |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-3fd7681c2ca0472d8e87458ca73229902025-08-20T03:52:39ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802020-01-01140110.3855/jidc.11940Commensal and virulent Escherichia coli strains of vaginal origin are reservoirs of resistance cassettes in class 1 integronsManalli Itzahaya Blancarte–Lagunas0Graciela Castro-Escarpulli1Armando Navarro-Ocaña2Gabriela Ibáñez-Cervantes3Laura Margarita Marquez-Valdelamar4José Misael Hernández-Carrillo5Juana Salazar-Salinas6Omar Fernando Mendoza-Vásquez7Gabriel Damazo-Hernández8Oscar Sosa-Hernández9Gregorio León-García10Monica Alethia Cureño-Díaz11Juan Manuel Bello-López12Laboratorio de Investigación Clínica y Ambiental. Departamento de Microbiología. Escuela Nacional de Ciencias Biológicas. Instituto Politécnico Nacional, Ciudad de México, MéxicoLaboratorio de Investigación Clínica y Ambiental. Departamento de Microbiología. Escuela Nacional de Ciencias Biológicas. Instituto Politécnico Nacional, Ciudad de México, MéxicoDepartamento de Salud Pública, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de México, MéxicoSección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Ciudad de México, MéxicoLaboratorio de Secuenciación Genómica de la Biodiversidad y de la Salud, Instituto de Biología, Universidad Nacional Autónoma de México, Ciudad de México, MéxicoLaboratorio de Vigilancia Epidemiológica, Subdirección de prevención y protección a la salud. Dirección médica del ISSSTE, Ciudad de México, MéxicoLaboratorio de Vigilancia Epidemiológica, Subdirección de prevención y protección a la salud. Dirección médica del ISSSTE, Ciudad de México, MéxicoLaboratorio de Vigilancia Epidemiológica, Subdirección de prevención y protección a la salud. Dirección médica del ISSSTE, Ciudad de México, MéxicoLaboratorio de Vigilancia Epidemiológica, Subdirección de prevención y protección a la salud. Dirección médica del ISSSTE, Ciudad de México, MéxicoHospital Juárez de México, Ciudad de MéxicoHospital de la Mujer, Ciudad de México, MéxicoHospital Juárez de México , Ciudad de México, MéxicoHospital Juárez de México , Ciudad de México, México Introduction: Antimicrobial resistance in Escherichia coli, one of the causal agents of aerobic vaginitis, leads to the persistence of the infection. The investigation of integrons acquires relevance, since they are elements that are responsible for the acquisition of resistance to antibiotics. The aim of this work was to describe the structural diversity of class 1 integrons in virulent and commensal strains of E. coli isolated from patients with vaginal infection. Methodology: Ninety-two strains of E. coli were isolated from patients with aerobic vaginitis. Resistance profile against 19 antibiotics and class 1 integrons were detected by PCR. The identity and arrangement of cassettes was determined by sequencing. ERIC-PCR assays were carried out in strains with identical arrays. Finally, genotyping by Clermont algorithm and serotyping were performed. Seventeen strains showed integrons located in plasmids. Results: Ten different gene cassette arrays were identified in 30 strains of E. coli. Cassettes corresponding to genes coding for adenylyltransferases (aadA), dihydrofolate reductases (dfrA), and oxacillinases (blaOXA) were detected. Array dfrA17-aadA5 was predominantly prevalent over the other arrays identified. Phylogenetic group A was the most predominant, followed by group B2 and D. Conclusions: This study demonstrates the presence of E. coli of vaginal origin carrying class 1 integrons, which are main genetic elements of capture of resistance genes, with the possibility of capturing new resistance cassettes. These evidences should serve for the modification of protocols in the diagnosis and treatment of aerobic vaginitis, and the development of policies for the rational use of antimicrobials. https://jidc.org/index.php/journal/article/view/11940Escherichia coliClass 1 integronsantimicrobial resistance |
| spellingShingle | Manalli Itzahaya Blancarte–Lagunas Graciela Castro-Escarpulli Armando Navarro-Ocaña Gabriela Ibáñez-Cervantes Laura Margarita Marquez-Valdelamar José Misael Hernández-Carrillo Juana Salazar-Salinas Omar Fernando Mendoza-Vásquez Gabriel Damazo-Hernández Oscar Sosa-Hernández Gregorio León-García Monica Alethia Cureño-Díaz Juan Manuel Bello-López Commensal and virulent Escherichia coli strains of vaginal origin are reservoirs of resistance cassettes in class 1 integrons Journal of Infection in Developing Countries Escherichia coli Class 1 integrons antimicrobial resistance |
| title | Commensal and virulent Escherichia coli strains of vaginal origin are reservoirs of resistance cassettes in class 1 integrons |
| title_full | Commensal and virulent Escherichia coli strains of vaginal origin are reservoirs of resistance cassettes in class 1 integrons |
| title_fullStr | Commensal and virulent Escherichia coli strains of vaginal origin are reservoirs of resistance cassettes in class 1 integrons |
| title_full_unstemmed | Commensal and virulent Escherichia coli strains of vaginal origin are reservoirs of resistance cassettes in class 1 integrons |
| title_short | Commensal and virulent Escherichia coli strains of vaginal origin are reservoirs of resistance cassettes in class 1 integrons |
| title_sort | commensal and virulent escherichia coli strains of vaginal origin are reservoirs of resistance cassettes in class 1 integrons |
| topic | Escherichia coli Class 1 integrons antimicrobial resistance |
| url | https://jidc.org/index.php/journal/article/view/11940 |
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