Culturing of Avian Embryos for Time-Lapse Imaging
Monitoring morphogenetic processes, at high resolution over time, has been a long-standing goal of many developmental cell biologists. It is critical to image cells in their natural environment whenever possible; however, imaging many warm-blooded vertebrates, especially mammals, is problematic. At...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2003-02-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/03342st01 |
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| _version_ | 1850152574957649920 |
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| author | Paul A. Rupp Brenda J. Rongish Andras Czirok Charles D. Little |
| author_facet | Paul A. Rupp Brenda J. Rongish Andras Czirok Charles D. Little |
| author_sort | Paul A. Rupp |
| collection | DOAJ |
| description | Monitoring morphogenetic processes, at high resolution over time, has been a long-standing goal of many developmental cell biologists. It is critical to image cells in their natural environment whenever possible; however, imaging many warm-blooded vertebrates, especially mammals, is problematic. At early stages of development, birds are ideal for imaging, since the avian body plan is very similar to that of mammals. We have devised a culturing technique that allows for the acquisition of high-resolution differential interference contrast and epifluorescence images of developing avian embryos in a 4-D (3-D + time) system. The resulting information, from intact embryos, is derived from an area encompassing several millimeters, at micrometer resolution for up to 30 h. |
| format | Article |
| id | doaj-art-3fd7258df52c49bb9a025d6af88a39b8 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2003-02-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-3fd7258df52c49bb9a025d6af88a39b82025-08-20T02:25:56ZengTaylor & Francis GroupBioTechniques0736-62051940-98182003-02-0134227427810.2144/03342st01Culturing of Avian Embryos for Time-Lapse ImagingPaul A. Rupp0Brenda J. Rongish1Andras Czirok2Charles D. Little31University of Kansas Medical Center, Kansas City, KS, USA1University of Kansas Medical Center, Kansas City, KS, USA1University of Kansas Medical Center, Kansas City, KS, USA1University of Kansas Medical Center, Kansas City, KS, USAMonitoring morphogenetic processes, at high resolution over time, has been a long-standing goal of many developmental cell biologists. It is critical to image cells in their natural environment whenever possible; however, imaging many warm-blooded vertebrates, especially mammals, is problematic. At early stages of development, birds are ideal for imaging, since the avian body plan is very similar to that of mammals. We have devised a culturing technique that allows for the acquisition of high-resolution differential interference contrast and epifluorescence images of developing avian embryos in a 4-D (3-D + time) system. The resulting information, from intact embryos, is derived from an area encompassing several millimeters, at micrometer resolution for up to 30 h.https://www.future-science.com/doi/10.2144/03342st01 |
| spellingShingle | Paul A. Rupp Brenda J. Rongish Andras Czirok Charles D. Little Culturing of Avian Embryos for Time-Lapse Imaging BioTechniques |
| title | Culturing of Avian Embryos for Time-Lapse Imaging |
| title_full | Culturing of Avian Embryos for Time-Lapse Imaging |
| title_fullStr | Culturing of Avian Embryos for Time-Lapse Imaging |
| title_full_unstemmed | Culturing of Avian Embryos for Time-Lapse Imaging |
| title_short | Culturing of Avian Embryos for Time-Lapse Imaging |
| title_sort | culturing of avian embryos for time lapse imaging |
| url | https://www.future-science.com/doi/10.2144/03342st01 |
| work_keys_str_mv | AT paularupp culturingofavianembryosfortimelapseimaging AT brendajrongish culturingofavianembryosfortimelapseimaging AT andrasczirok culturingofavianembryosfortimelapseimaging AT charlesdlittle culturingofavianembryosfortimelapseimaging |