Response of <i>Arabidopsis thaliana</i> to Flooding with Physical Flow
Flooding causes severe yield losses worldwide, making it urgent to enhance crop tolerance to this stress. Since natural flooding often involves physical flow, we hypothesized that the effects of submergence on plants could change when combined with physical flow. In this study, we analyzed the growt...
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2024-12-01
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| author | Momoko Kaji Kazuma Katano Taufika Islam Anee Hiroshi Nitta Ryotaro Yamaji Rio Shimizu Shunsuke Shigaki Hiroyuki Suzuki Nobuhiro Suzuki |
| author_facet | Momoko Kaji Kazuma Katano Taufika Islam Anee Hiroshi Nitta Ryotaro Yamaji Rio Shimizu Shunsuke Shigaki Hiroyuki Suzuki Nobuhiro Suzuki |
| author_sort | Momoko Kaji |
| collection | DOAJ |
| description | Flooding causes severe yield losses worldwide, making it urgent to enhance crop tolerance to this stress. Since natural flooding often involves physical flow, we hypothesized that the effects of submergence on plants could change when combined with physical flow. In this study, we analyzed the growth and transcriptome of <i>Arabidopsis thaliana</i> exposed to submergence or flooding with physical flow. Plants exposed to flooding with physical flow had smaller rosette diameters, especially at faster flow rates. Transcriptome analysis revealed that “defense response” transcripts were highly up-regulated in response to flooding with physical flow. In addition, up-regulation of transcripts encoding ROS-producing enzymes, SA synthesis, JA synthesis, and ethylene signaling was more pronounced under flooding with physical flow when compared to submergence. Although H<sub>2</sub>O<sub>2</sub> accumulation changed in response to submergence or flooding with physical flow, it did not lead to lipid peroxidation, suggesting a role for ROS as signaling molecules under these conditions. Multiple regression analysis indicated possible links between rosette diameter under flooding with physical flow and the expression of <i>Rboh</i>s and SA synthesis transcripts. These findings suggest that pathogen defense responses, regulated by SA and ROS signaling, play crucial roles in plant responses to flooding with physical flow. |
| format | Article |
| id | doaj-art-3fd1b6bf0d1e45a2a8286f85af5ce018 |
| institution | OA Journals |
| issn | 2223-7747 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-3fd1b6bf0d1e45a2a8286f85af5ce0182025-08-20T02:01:16ZengMDPI AGPlants2223-77472024-12-011324350810.3390/plants13243508Response of <i>Arabidopsis thaliana</i> to Flooding with Physical FlowMomoko Kaji0Kazuma Katano1Taufika Islam Anee2Hiroshi Nitta3Ryotaro Yamaji4Rio Shimizu5Shunsuke Shigaki6Hiroyuki Suzuki7Nobuhiro Suzuki8National Institute of Technology, Ishikawa College, Tsubata 929-0392, Ishikawa, JapanCollege of Life Sciences, Ritsumeikan University, Kusatsu 525-8577, Shiga, JapanDepartment of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, Chiyoda, Tokyo 102-8554, JapanNational Institute of Technology, Ishikawa College, Tsubata 929-0392, Ishikawa, JapanNational Institute of Technology, Ishikawa College, Tsubata 929-0392, Ishikawa, JapanDepartment of Agronomy, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka 1207, BangladeshPrinciples of Informatics Research Division, National Institute of Informatics, Chiyoda, Tokyo 101-8430, JapanDepartment of Civil and Environmental Engineering, Faculty of Engineering, Hokkai-Gakuen University, Sapporo 062-8605, Hokkaido, JapanDepartment of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, Chiyoda, Tokyo 102-8554, JapanFlooding causes severe yield losses worldwide, making it urgent to enhance crop tolerance to this stress. Since natural flooding often involves physical flow, we hypothesized that the effects of submergence on plants could change when combined with physical flow. In this study, we analyzed the growth and transcriptome of <i>Arabidopsis thaliana</i> exposed to submergence or flooding with physical flow. Plants exposed to flooding with physical flow had smaller rosette diameters, especially at faster flow rates. Transcriptome analysis revealed that “defense response” transcripts were highly up-regulated in response to flooding with physical flow. In addition, up-regulation of transcripts encoding ROS-producing enzymes, SA synthesis, JA synthesis, and ethylene signaling was more pronounced under flooding with physical flow when compared to submergence. Although H<sub>2</sub>O<sub>2</sub> accumulation changed in response to submergence or flooding with physical flow, it did not lead to lipid peroxidation, suggesting a role for ROS as signaling molecules under these conditions. Multiple regression analysis indicated possible links between rosette diameter under flooding with physical flow and the expression of <i>Rboh</i>s and SA synthesis transcripts. These findings suggest that pathogen defense responses, regulated by SA and ROS signaling, play crucial roles in plant responses to flooding with physical flow.https://www.mdpi.com/2223-7747/13/24/3508floodingjasmonic acid (JA)pathogen defensephysical flowrespiratory burst oxidase homologue (RBOH)salicylic acid (SA) |
| spellingShingle | Momoko Kaji Kazuma Katano Taufika Islam Anee Hiroshi Nitta Ryotaro Yamaji Rio Shimizu Shunsuke Shigaki Hiroyuki Suzuki Nobuhiro Suzuki Response of <i>Arabidopsis thaliana</i> to Flooding with Physical Flow Plants flooding jasmonic acid (JA) pathogen defense physical flow respiratory burst oxidase homologue (RBOH) salicylic acid (SA) |
| title | Response of <i>Arabidopsis thaliana</i> to Flooding with Physical Flow |
| title_full | Response of <i>Arabidopsis thaliana</i> to Flooding with Physical Flow |
| title_fullStr | Response of <i>Arabidopsis thaliana</i> to Flooding with Physical Flow |
| title_full_unstemmed | Response of <i>Arabidopsis thaliana</i> to Flooding with Physical Flow |
| title_short | Response of <i>Arabidopsis thaliana</i> to Flooding with Physical Flow |
| title_sort | response of i arabidopsis thaliana i to flooding with physical flow |
| topic | flooding jasmonic acid (JA) pathogen defense physical flow respiratory burst oxidase homologue (RBOH) salicylic acid (SA) |
| url | https://www.mdpi.com/2223-7747/13/24/3508 |
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