CRISPR-Cas based precision genome editing: current advances and associated challenges in crop improvement and trait enhancement

The CRISPR-Cas system has revolutionized precision genome editing and opened up enormous prospects for crop improvement and trait enhancement. This review covers recent advances related to the application of CRISPR-Cas in agricultural biotechnology, with an emphasis on its potential to contribute t...

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Main Authors: Samanwaya Niraula, Rasik Khanal, Nischal Ghimire
Format: Article
Language:English
Published: University of Sarajevo, Institute for Genetic Engineering and Biotechnology 2024-12-01
Series:Genetics & Applications
Subjects:
Online Access:https://genapp.ba/editions/index.php/journal/article/view/224
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author Samanwaya Niraula
Rasik Khanal
Nischal Ghimire
author_facet Samanwaya Niraula
Rasik Khanal
Nischal Ghimire
author_sort Samanwaya Niraula
collection DOAJ
description The CRISPR-Cas system has revolutionized precision genome editing and opened up enormous prospects for crop improvement and trait enhancement. This review covers recent advances related to the application of CRISPR-Cas in agricultural biotechnology, with an emphasis on its potential to contribute to solving critical issues of food security and sustainability. Global population is expected to reach 10 billion by 2050, presenting a challenge that demands new approaches to raise agricultural productivity. The review investigates the mechanisms by which CRISPR-Cas systems, especially Cas9 and Cas12a, facilitate precise alterations in plant genomes, leading to augmented resilience against both biological and environmental challenges. Significant accomplishments comprise increased drought resistance, enhanced disease tolerance, and improved nutritional quality in agricultural species such as wheat, maize, and soybean. However, the application of CRISPR-Cas technology encounters challenges, including regulatory hurdles and public acceptance. This paper underlines modern achievements together with future possibilities, at the same time paying much attention to the connected challenges and emphasizing the crucial role of CRISPR-Cas technology for the advancement of sustainable agriculture. Using such a powerful tool, scientists aim at creating resistant crop species that will be able to meet the needs of the growing population while providing food safety and quality.
format Article
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institution OA Journals
issn 2566-2937
2566-431X
language English
publishDate 2024-12-01
publisher University of Sarajevo, Institute for Genetic Engineering and Biotechnology
record_format Article
series Genetics & Applications
spelling doaj-art-3fd143165f404409abcbed59254c70ae2025-08-20T02:32:33ZengUniversity of Sarajevo, Institute for Genetic Engineering and BiotechnologyGenetics & Applications2566-29372566-431X2024-12-018210.31383/ga.vol8iss2ga05CRISPR-Cas based precision genome editing: current advances and associated challenges in crop improvement and trait enhancementSamanwaya Niraula0Rasik Khanal1Nischal Ghimire2Institute of Agriculture and Animal science, Lamjung Campus, Tribhuvan University, NepalInstitute of Agriculture and Animal science, Lamjung Campus, Tribhuvan University, NepalInstitute of Agriculture and Animal science, Lamjung Campus, Tribhuvan University, Nepal The CRISPR-Cas system has revolutionized precision genome editing and opened up enormous prospects for crop improvement and trait enhancement. This review covers recent advances related to the application of CRISPR-Cas in agricultural biotechnology, with an emphasis on its potential to contribute to solving critical issues of food security and sustainability. Global population is expected to reach 10 billion by 2050, presenting a challenge that demands new approaches to raise agricultural productivity. The review investigates the mechanisms by which CRISPR-Cas systems, especially Cas9 and Cas12a, facilitate precise alterations in plant genomes, leading to augmented resilience against both biological and environmental challenges. Significant accomplishments comprise increased drought resistance, enhanced disease tolerance, and improved nutritional quality in agricultural species such as wheat, maize, and soybean. However, the application of CRISPR-Cas technology encounters challenges, including regulatory hurdles and public acceptance. This paper underlines modern achievements together with future possibilities, at the same time paying much attention to the connected challenges and emphasizing the crucial role of CRISPR-Cas technology for the advancement of sustainable agriculture. Using such a powerful tool, scientists aim at creating resistant crop species that will be able to meet the needs of the growing population while providing food safety and quality. https://genapp.ba/editions/index.php/journal/article/view/224CRISPR/Cas9plant genome editingcrop improvementagricultural biotechnologycurrent statusfood security
spellingShingle Samanwaya Niraula
Rasik Khanal
Nischal Ghimire
CRISPR-Cas based precision genome editing: current advances and associated challenges in crop improvement and trait enhancement
Genetics & Applications
CRISPR/Cas9
plant genome editing
crop improvement
agricultural biotechnology
current status
food security
title CRISPR-Cas based precision genome editing: current advances and associated challenges in crop improvement and trait enhancement
title_full CRISPR-Cas based precision genome editing: current advances and associated challenges in crop improvement and trait enhancement
title_fullStr CRISPR-Cas based precision genome editing: current advances and associated challenges in crop improvement and trait enhancement
title_full_unstemmed CRISPR-Cas based precision genome editing: current advances and associated challenges in crop improvement and trait enhancement
title_short CRISPR-Cas based precision genome editing: current advances and associated challenges in crop improvement and trait enhancement
title_sort crispr cas based precision genome editing current advances and associated challenges in crop improvement and trait enhancement
topic CRISPR/Cas9
plant genome editing
crop improvement
agricultural biotechnology
current status
food security
url https://genapp.ba/editions/index.php/journal/article/view/224
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AT rasikkhanal crisprcasbasedprecisiongenomeeditingcurrentadvancesandassociatedchallengesincropimprovementandtraitenhancement
AT nischalghimire crisprcasbasedprecisiongenomeeditingcurrentadvancesandassociatedchallengesincropimprovementandtraitenhancement